Evaluation of Recombinant Antibody Production Efficiency in CHO Cells with Sleeping Beauty Transposon Vector System
Chinese hamster ovary (CHO) mammalian cell lines are widely used as cell platforms in biopharmaceutical productions. Different transfection systems are employed for the integration of the target gene cassette into the cell genome and have limitations, such as (i) the integration region in the genome...
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Çanakkale Onsekiz Mart University
2024-09-01
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| Series: | Journal of Advanced Research in Natural and Applied Sciences |
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| Online Access: | https://dergipark.org.tr/en/download/article-file/3917162 |
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| author | Pelin Kolçak Yaşlı Seda Kulabaş Evren Doruk Engin |
| author_facet | Pelin Kolçak Yaşlı Seda Kulabaş Evren Doruk Engin |
| author_sort | Pelin Kolçak Yaşlı |
| collection | DOAJ |
| description | Chinese hamster ovary (CHO) mammalian cell lines are widely used as cell platforms in biopharmaceutical productions. Different transfection systems are employed for the integration of the target gene cassette into the cell genome and have limitations, such as (i) the integration region in the genome, (ii) the size of the target cassette, and (iii) long selection periods for stable expression. Transposon systems can be utilized to overcome the limitations mentioned in the efficient production of commercially significant recombinant proteins. This study aims to demonstrate the differences in production potential and selection periods by using a specially designed vector system for random genome integration in CHODG44 DHFR -/- cells and the Sleeping Beauty (SB) transposon system. In this context, the optimal transfer ratio between the donor and the helper plasmid was determined for the most efficient co-transfection in the SB transposon system. According to the results, the pools obtained using the SB transposon system had titers ranging from 1300 to 2600 mg/L in 13-day fed-batch studies, while the pool obtained using the random transfer system had a titer of 0.056 mg/L. Additionally, stable cell pools obtained using the transposon system underwent selection in a short period of 52 days, compared to over 100 days for the pool obtained through random transfer. Considering all these results together, it is demonstrated that stable CHO pools obtained using the optimal SB transposon system can achieve high-efficiency monoclonal antibody production in a short period, making it an optimal production platform in the biopharmaceutical field. |
| format | Article |
| id | doaj-art-8f3f13cab2f04da986499616f2b7cfd5 |
| institution | Kabale University |
| issn | 2757-5195 |
| language | English |
| publishDate | 2024-09-01 |
| publisher | Çanakkale Onsekiz Mart University |
| record_format | Article |
| series | Journal of Advanced Research in Natural and Applied Sciences |
| spelling | doaj-art-8f3f13cab2f04da986499616f2b7cfd52025-02-05T18:13:02ZengÇanakkale Onsekiz Mart UniversityJournal of Advanced Research in Natural and Applied Sciences2757-51952024-09-0110357158610.28979/jarnas.1481069453Evaluation of Recombinant Antibody Production Efficiency in CHO Cells with Sleeping Beauty Transposon Vector SystemPelin Kolçak Yaşlı0https://orcid.org/0000-0003-4724-5796Seda Kulabaş1https://orcid.org/0000-0003-1258-8019Evren Doruk Engin2https://orcid.org/0000-0001-9209-8858ANKARA ÜNİVERSİTESİ, BİYOTEKNOLOJİ ENSTİTÜSÜÇANAKKALE ONSEKİZ MART ÜNİVERSİTESİ, TIP FAKÜLTESİANKARA ÜNİVERSİTESİ, BİYOTEKNOLOJİ ENSTİTÜSÜChinese hamster ovary (CHO) mammalian cell lines are widely used as cell platforms in biopharmaceutical productions. Different transfection systems are employed for the integration of the target gene cassette into the cell genome and have limitations, such as (i) the integration region in the genome, (ii) the size of the target cassette, and (iii) long selection periods for stable expression. Transposon systems can be utilized to overcome the limitations mentioned in the efficient production of commercially significant recombinant proteins. This study aims to demonstrate the differences in production potential and selection periods by using a specially designed vector system for random genome integration in CHODG44 DHFR -/- cells and the Sleeping Beauty (SB) transposon system. In this context, the optimal transfer ratio between the donor and the helper plasmid was determined for the most efficient co-transfection in the SB transposon system. According to the results, the pools obtained using the SB transposon system had titers ranging from 1300 to 2600 mg/L in 13-day fed-batch studies, while the pool obtained using the random transfer system had a titer of 0.056 mg/L. Additionally, stable cell pools obtained using the transposon system underwent selection in a short period of 52 days, compared to over 100 days for the pool obtained through random transfer. Considering all these results together, it is demonstrated that stable CHO pools obtained using the optimal SB transposon system can achieve high-efficiency monoclonal antibody production in a short period, making it an optimal production platform in the biopharmaceutical field.https://dergipark.org.tr/en/download/article-file/3917162sleeping beauty transposongene integrationexpression levelcho cellsindustrial production |
| spellingShingle | Pelin Kolçak Yaşlı Seda Kulabaş Evren Doruk Engin Evaluation of Recombinant Antibody Production Efficiency in CHO Cells with Sleeping Beauty Transposon Vector System Journal of Advanced Research in Natural and Applied Sciences sleeping beauty transposon gene integration expression level cho cells industrial production |
| title | Evaluation of Recombinant Antibody Production Efficiency in CHO Cells with Sleeping Beauty Transposon Vector System |
| title_full | Evaluation of Recombinant Antibody Production Efficiency in CHO Cells with Sleeping Beauty Transposon Vector System |
| title_fullStr | Evaluation of Recombinant Antibody Production Efficiency in CHO Cells with Sleeping Beauty Transposon Vector System |
| title_full_unstemmed | Evaluation of Recombinant Antibody Production Efficiency in CHO Cells with Sleeping Beauty Transposon Vector System |
| title_short | Evaluation of Recombinant Antibody Production Efficiency in CHO Cells with Sleeping Beauty Transposon Vector System |
| title_sort | evaluation of recombinant antibody production efficiency in cho cells with sleeping beauty transposon vector system |
| topic | sleeping beauty transposon gene integration expression level cho cells industrial production |
| url | https://dergipark.org.tr/en/download/article-file/3917162 |
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