Cell Therapy for Chemically Induced Ovarian Failure in Mice
Cell therapy has been linked to an unexplained return of ovarian function and fertility in some cancer survivors. Studies modeling this in mice have shown that cells transplantation generates donor-derived oocytes in chemotherapy-treated recipients. This study was conducted to further clarify the im...
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| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2014/720753 |
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| _version_ | 1832565659361345536 |
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| author | Paula Terraciano Tuane Garcez Laura Ayres Isabel Durli Melchiani Baggio Cristiana Palma Kuhl Claudia Laurino Eduardo Passos Ana Helena Paz Elizabeth Cirne-Lima |
| author_facet | Paula Terraciano Tuane Garcez Laura Ayres Isabel Durli Melchiani Baggio Cristiana Palma Kuhl Claudia Laurino Eduardo Passos Ana Helena Paz Elizabeth Cirne-Lima |
| author_sort | Paula Terraciano |
| collection | DOAJ |
| description | Cell therapy has been linked to an unexplained return of ovarian function and fertility in some cancer survivors. Studies modeling this in mice have shown that cells transplantation generates donor-derived oocytes in chemotherapy-treated recipients. This study was conducted to further clarify the impact of cell transplantation from different sources on female reproductive function after chemotherapy using a preclinical mouse model. Methods. Female mice were administered 7.5 mg/kg cisplatin followed by cell transplantation (one week later) using GFP+ female cell donors. For cell tracking, adipose derived stem cell GFP+ (ADSC), female germline stem cell GFP+/MVH+ (FGSC), or ovary cell suspension GFP+ mice were transplanted into cisplatin-treated wild-type recipients. After 7 or 14 days animals were killed and histological analysis, IHQ for GFP cells, and ELISA for estradiol were performed. Results. Histological examinations showed that ADSC, ovary cell suspension, and FGSC transplant increase the number of follicles with apparent normal structure in the cells recipient group euthanized on day 7. Cell tracking showed GFP+ samples 7 days after transplant. Conclusion. These data suggest that intraovarian injection of ADSCs and FGSC into mice with chemotherapy-induced ovarian failure diminished the damage caused by cisplatin. |
| format | Article |
| id | doaj-art-8ef0b1a52bd24c7b85c53c6ae8426f8e |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-8ef0b1a52bd24c7b85c53c6ae8426f8e2025-02-03T01:06:58ZengWileyStem Cells International1687-966X1687-96782014-01-01201410.1155/2014/720753720753Cell Therapy for Chemically Induced Ovarian Failure in MicePaula Terraciano0Tuane Garcez1Laura Ayres2Isabel Durli3Melchiani Baggio4Cristiana Palma Kuhl5Claudia Laurino6Eduardo Passos7Ana Helena Paz8Elizabeth Cirne-Lima9Hospital de Clínicas de Porto Alegre, 90035-903 Porto Alegre, RS, BrazilHospital de Clínicas de Porto Alegre, 90035-903 Porto Alegre, RS, BrazilHospital de Clínicas de Porto Alegre, 90035-903 Porto Alegre, RS, BrazilHospital de Clínicas de Porto Alegre, 90035-903 Porto Alegre, RS, BrazilHospital de Clínicas de Porto Alegre, 90035-903 Porto Alegre, RS, BrazilHospital de Clínicas de Porto Alegre, 90035-903 Porto Alegre, RS, BrazilHospital de Clínicas de Porto Alegre, 90035-903 Porto Alegre, RS, BrazilHospital de Clínicas de Porto Alegre, 90035-903 Porto Alegre, RS, BrazilHospital de Clínicas de Porto Alegre, 90035-903 Porto Alegre, RS, BrazilHospital de Clínicas de Porto Alegre, 90035-903 Porto Alegre, RS, BrazilCell therapy has been linked to an unexplained return of ovarian function and fertility in some cancer survivors. Studies modeling this in mice have shown that cells transplantation generates donor-derived oocytes in chemotherapy-treated recipients. This study was conducted to further clarify the impact of cell transplantation from different sources on female reproductive function after chemotherapy using a preclinical mouse model. Methods. Female mice were administered 7.5 mg/kg cisplatin followed by cell transplantation (one week later) using GFP+ female cell donors. For cell tracking, adipose derived stem cell GFP+ (ADSC), female germline stem cell GFP+/MVH+ (FGSC), or ovary cell suspension GFP+ mice were transplanted into cisplatin-treated wild-type recipients. After 7 or 14 days animals were killed and histological analysis, IHQ for GFP cells, and ELISA for estradiol were performed. Results. Histological examinations showed that ADSC, ovary cell suspension, and FGSC transplant increase the number of follicles with apparent normal structure in the cells recipient group euthanized on day 7. Cell tracking showed GFP+ samples 7 days after transplant. Conclusion. These data suggest that intraovarian injection of ADSCs and FGSC into mice with chemotherapy-induced ovarian failure diminished the damage caused by cisplatin.http://dx.doi.org/10.1155/2014/720753 |
| spellingShingle | Paula Terraciano Tuane Garcez Laura Ayres Isabel Durli Melchiani Baggio Cristiana Palma Kuhl Claudia Laurino Eduardo Passos Ana Helena Paz Elizabeth Cirne-Lima Cell Therapy for Chemically Induced Ovarian Failure in Mice Stem Cells International |
| title | Cell Therapy for Chemically Induced Ovarian Failure in Mice |
| title_full | Cell Therapy for Chemically Induced Ovarian Failure in Mice |
| title_fullStr | Cell Therapy for Chemically Induced Ovarian Failure in Mice |
| title_full_unstemmed | Cell Therapy for Chemically Induced Ovarian Failure in Mice |
| title_short | Cell Therapy for Chemically Induced Ovarian Failure in Mice |
| title_sort | cell therapy for chemically induced ovarian failure in mice |
| url | http://dx.doi.org/10.1155/2014/720753 |
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