The Impact of Levothyroxine and Testosterone Administration on Bladder Contractility in the Rat Model of Propylthiouracil-Induced Hypothyroidism
Objective: To investigate the effects of testosterone (T) treatment, with or without levothyroxine, the most widely used and least effective medication for managing hypothyroidism, on the functional and histological changes in propylthiouracil (PTU)- induced hypothyroid rat bladders. Methods: Mal...
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2024-07-01
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| Series: | Urology Research and Practice |
| Online Access: | https://urologyresearchandpractice.org/en/the-impact-of-levothyroxine-and-testosterone-administration-on-bladder-contractility-in-the-rat-model-of-propylthiouracil-induced-hypothyroidism-133952 |
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| author | Didem Yılmaz Oral Berna Güven Ciloğlu Serap Gür |
| author_facet | Didem Yılmaz Oral Berna Güven Ciloğlu Serap Gür |
| author_sort | Didem Yılmaz Oral |
| collection | DOAJ |
| description | Objective: To investigate the effects of testosterone (T) treatment, with or without levothyroxine, the most widely used and least effective medication for managing hypothyroidism, on the functional and histological changes in propylthiouracil (PTU)- induced hypothyroid rat bladders.
Methods: Male rats (n=35) were split into control, hypothyroid, hypothyroid rats treated with levothyroxine (20 µg/kg/day, oral, 2-weeks), hypothyroid rats treated with Sustanon (10 mg/kg,iIM, once/week, 2-weeks), and hypothyroid rats treated with combined treatment groups. Hypothyroidism was induced by PTU (0.05% in drinking water, 6 weeks). The serum concentration of triiodothyronine (T3), thyroxine (T4), total T, and the detrusor muscle’s contractile responses were determined. A morphological study was conducted using Masson trichrome staining.
Results: Triiodothyronine, T4, and T levels were considerably reduced in rats with hypothyroidism; however, these hormones were restored by levothyroxine and Sustanon. Compared to controls, the combination therapy improved the ratio of smooth muscle to collagen and the contractile responses to carbachol, electrical field stimulation, and adenosine triphosphate in the hypothyroid bladder.
Conclusion: The authors’ research suggests that hypothyroidism may affect the contractility and morphology of the bladder. In males with hypothyroidism and urogenital system dysfunction, combination therapy with thyroid hormones and T has a major impact on repairing detrusor smooth muscle contractility and bladder histomorphology. |
| format | Article |
| id | doaj-art-8ecdb421ae3b49b0914d2660fd555fc5 |
| institution | Kabale University |
| issn | 2980-1478 |
| language | English |
| publishDate | 2024-07-01 |
| publisher | AVES |
| record_format | Article |
| series | Urology Research and Practice |
| spelling | doaj-art-8ecdb421ae3b49b0914d2660fd555fc52025-01-31T14:14:58ZengAVESUrology Research and Practice2980-14782024-07-0150424725210.5152/tud.2025.24099The Impact of Levothyroxine and Testosterone Administration on Bladder Contractility in the Rat Model of Propylthiouracil-Induced HypothyroidismDidem Yılmaz Oral0https://orcid.org/0000-0002-9515-0698Berna Güven Ciloğlu1https://orcid.org/0000-0001-9181-3741Serap Gür2https://orcid.org/0000-0002-1730-7282Department of Pharmacology, Çukurova University, Faculty of Pharmacy, Adana, TürkiyeDepartment of Pharmacology, Ankara University, Faculty of Pharmacy, Ankara, TürkiyeDepartment of Pharmacology, Ankara University, Faculty of Pharmacy, Ankara, TürkiyeObjective: To investigate the effects of testosterone (T) treatment, with or without levothyroxine, the most widely used and least effective medication for managing hypothyroidism, on the functional and histological changes in propylthiouracil (PTU)- induced hypothyroid rat bladders. Methods: Male rats (n=35) were split into control, hypothyroid, hypothyroid rats treated with levothyroxine (20 µg/kg/day, oral, 2-weeks), hypothyroid rats treated with Sustanon (10 mg/kg,iIM, once/week, 2-weeks), and hypothyroid rats treated with combined treatment groups. Hypothyroidism was induced by PTU (0.05% in drinking water, 6 weeks). The serum concentration of triiodothyronine (T3), thyroxine (T4), total T, and the detrusor muscle’s contractile responses were determined. A morphological study was conducted using Masson trichrome staining. Results: Triiodothyronine, T4, and T levels were considerably reduced in rats with hypothyroidism; however, these hormones were restored by levothyroxine and Sustanon. Compared to controls, the combination therapy improved the ratio of smooth muscle to collagen and the contractile responses to carbachol, electrical field stimulation, and adenosine triphosphate in the hypothyroid bladder. Conclusion: The authors’ research suggests that hypothyroidism may affect the contractility and morphology of the bladder. In males with hypothyroidism and urogenital system dysfunction, combination therapy with thyroid hormones and T has a major impact on repairing detrusor smooth muscle contractility and bladder histomorphology.https://urologyresearchandpractice.org/en/the-impact-of-levothyroxine-and-testosterone-administration-on-bladder-contractility-in-the-rat-model-of-propylthiouracil-induced-hypothyroidism-133952 |
| spellingShingle | Didem Yılmaz Oral Berna Güven Ciloğlu Serap Gür The Impact of Levothyroxine and Testosterone Administration on Bladder Contractility in the Rat Model of Propylthiouracil-Induced Hypothyroidism Urology Research and Practice |
| title | The Impact of Levothyroxine and Testosterone Administration on Bladder Contractility in the Rat Model of Propylthiouracil-Induced Hypothyroidism |
| title_full | The Impact of Levothyroxine and Testosterone Administration on Bladder Contractility in the Rat Model of Propylthiouracil-Induced Hypothyroidism |
| title_fullStr | The Impact of Levothyroxine and Testosterone Administration on Bladder Contractility in the Rat Model of Propylthiouracil-Induced Hypothyroidism |
| title_full_unstemmed | The Impact of Levothyroxine and Testosterone Administration on Bladder Contractility in the Rat Model of Propylthiouracil-Induced Hypothyroidism |
| title_short | The Impact of Levothyroxine and Testosterone Administration on Bladder Contractility in the Rat Model of Propylthiouracil-Induced Hypothyroidism |
| title_sort | impact of levothyroxine and testosterone administration on bladder contractility in the rat model of propylthiouracil induced hypothyroidism |
| url | https://urologyresearchandpractice.org/en/the-impact-of-levothyroxine-and-testosterone-administration-on-bladder-contractility-in-the-rat-model-of-propylthiouracil-induced-hypothyroidism-133952 |
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