Detection and Characterization of the Metabolites of Ciwujianoside B in Rats Based on UPLC-Fusion Lumos Orbitrap Mass Spectrometry
We previously conducted a systematic study on the metabolic process and products of hederasaponin B in rats. We hypothesized that the sugar chain structures play a key role in the metabolism of triterpenoid saponins. To verify this hypothesis, we conducted metabolic research on ciwujianoside B ascri...
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| Format: | Article |
| Language: | English |
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Wiley
2024-01-01
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| Series: | Journal of Analytical Methods in Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2024/3187511 |
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| author | Wan-Ru Dong Xue Gao Chen-Xue Li Yan Song Jun-Hong Chai Jun Liang |
| author_facet | Wan-Ru Dong Xue Gao Chen-Xue Li Yan Song Jun-Hong Chai Jun Liang |
| author_sort | Wan-Ru Dong |
| collection | DOAJ |
| description | We previously conducted a systematic study on the metabolic process and products of hederasaponin B in rats. We hypothesized that the sugar chain structures play a key role in the metabolism of triterpenoid saponins. To verify this hypothesis, we conducted metabolic research on ciwujianoside B ascribed to the same sugar chains and a distinct aglycone and compared it with hederasaponin B. Specifically, we collected feces, urine, and plasma of rats after gavage with ciwujianoside B and identified 42 metabolites by UPLC-Fusion Lumos Orbitrap mass spectrometry. Finally, ciwujianoside B metabolism and hederasaponin B metabolism were compared, reaching the following conclusions: (i) more than 40 metabolites were identified in both, with the majority of metabolites identified in feces; (ii) the corresponding metabolic pathways in vivo were basically similar, including deglycosylation, acetylation, hydroxylation, glucuronidation, oxidation, and glycosylation; and (iii) deglycosylation was considered the main metabolic reaction, and its metabolites accounted for approximately 50% of all metabolites. Overall, this study provides a foundation for further research on the metabolism of triterpenoid saponins. |
| format | Article |
| id | doaj-art-8eb1c77e995c45268f465f77ff74c3ab |
| institution | Kabale University |
| issn | 2090-8873 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Analytical Methods in Chemistry |
| spelling | doaj-art-8eb1c77e995c45268f465f77ff74c3ab2025-02-03T05:56:56ZengWileyJournal of Analytical Methods in Chemistry2090-88732024-01-01202410.1155/2024/3187511Detection and Characterization of the Metabolites of Ciwujianoside B in Rats Based on UPLC-Fusion Lumos Orbitrap Mass SpectrometryWan-Ru Dong0Xue Gao1Chen-Xue Li2Yan Song3Jun-Hong Chai4Jun Liang5Key Laboratory of Basic and Application Research of Beiyao (Heilongjiang University of Chinese Medicine)Key Laboratory of Basic and Application Research of Beiyao (Heilongjiang University of Chinese Medicine)Key Laboratory of Basic and Application Research of Beiyao (Heilongjiang University of Chinese Medicine)Key Laboratory of Basic and Application Research of Beiyao (Heilongjiang University of Chinese Medicine)Key Laboratory of Basic and Application Research of Beiyao (Heilongjiang University of Chinese Medicine)Key Laboratory of Basic and Application Research of Beiyao (Heilongjiang University of Chinese Medicine)We previously conducted a systematic study on the metabolic process and products of hederasaponin B in rats. We hypothesized that the sugar chain structures play a key role in the metabolism of triterpenoid saponins. To verify this hypothesis, we conducted metabolic research on ciwujianoside B ascribed to the same sugar chains and a distinct aglycone and compared it with hederasaponin B. Specifically, we collected feces, urine, and plasma of rats after gavage with ciwujianoside B and identified 42 metabolites by UPLC-Fusion Lumos Orbitrap mass spectrometry. Finally, ciwujianoside B metabolism and hederasaponin B metabolism were compared, reaching the following conclusions: (i) more than 40 metabolites were identified in both, with the majority of metabolites identified in feces; (ii) the corresponding metabolic pathways in vivo were basically similar, including deglycosylation, acetylation, hydroxylation, glucuronidation, oxidation, and glycosylation; and (iii) deglycosylation was considered the main metabolic reaction, and its metabolites accounted for approximately 50% of all metabolites. Overall, this study provides a foundation for further research on the metabolism of triterpenoid saponins.http://dx.doi.org/10.1155/2024/3187511 |
| spellingShingle | Wan-Ru Dong Xue Gao Chen-Xue Li Yan Song Jun-Hong Chai Jun Liang Detection and Characterization of the Metabolites of Ciwujianoside B in Rats Based on UPLC-Fusion Lumos Orbitrap Mass Spectrometry Journal of Analytical Methods in Chemistry |
| title | Detection and Characterization of the Metabolites of Ciwujianoside B in Rats Based on UPLC-Fusion Lumos Orbitrap Mass Spectrometry |
| title_full | Detection and Characterization of the Metabolites of Ciwujianoside B in Rats Based on UPLC-Fusion Lumos Orbitrap Mass Spectrometry |
| title_fullStr | Detection and Characterization of the Metabolites of Ciwujianoside B in Rats Based on UPLC-Fusion Lumos Orbitrap Mass Spectrometry |
| title_full_unstemmed | Detection and Characterization of the Metabolites of Ciwujianoside B in Rats Based on UPLC-Fusion Lumos Orbitrap Mass Spectrometry |
| title_short | Detection and Characterization of the Metabolites of Ciwujianoside B in Rats Based on UPLC-Fusion Lumos Orbitrap Mass Spectrometry |
| title_sort | detection and characterization of the metabolites of ciwujianoside b in rats based on uplc fusion lumos orbitrap mass spectrometry |
| url | http://dx.doi.org/10.1155/2024/3187511 |
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