Quantification of mRNA Using Competitive RTPCR with Standard-Curve Methodology
The use of reverse transcription polymerase chain reaction (RT-PCR) with internal RNA competitive standards (competitors) provides a means for measuring absolute amounts of mRNA transcripts in small numbers of cells. Most quantitative competitive (QC)-RT-PCR methods require analysis of multiple reac...
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| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
1996-11-01
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| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/96215st04 |
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