Clinically relevant radioresistant cells exhibit resistance to HO by decreasing internal HO and lipid peroxidation
Radiation therapy is one of the choices to treat malignant tumors. In radiation therapy, existence of radiation-resistant cell is a major problem to overcome. We established clinically relevant radioresistant cells that had been obtained by exposing to 2 Gy/day X-rays for more than 30 days. These ce...
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| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2018-09-01
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| Series: | Tumor Biology |
| Online Access: | https://doi.org/10.1177/1010428318799250 |
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| _version_ | 1849411201743716352 |
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| author | Kazuo Tomita Yoshikazu Kuwahara Yuko Takashi Kento Igarashi Taisuke Nagasawa Hideki Nabika Akihiro Kurimasa Manabu Fukumoto Yoshihiro Nishitani Tomoaki Sato |
| author_facet | Kazuo Tomita Yoshikazu Kuwahara Yuko Takashi Kento Igarashi Taisuke Nagasawa Hideki Nabika Akihiro Kurimasa Manabu Fukumoto Yoshihiro Nishitani Tomoaki Sato |
| author_sort | Kazuo Tomita |
| collection | DOAJ |
| description | Radiation therapy is one of the choices to treat malignant tumors. In radiation therapy, existence of radiation-resistant cell is a major problem to overcome. We established clinically relevant radioresistant cells that had been obtained by exposing to 2 Gy/day X-rays for more than 30 days. These cells are resistant to 2 Gy/day X-ray exposure and anticancer agents. However, the underlying resistance mechanism remains unclear. We investigated the resistance of clinically relevant radioresistant cells to hydrogen peroxide (H 2 O 2 ), confirming a degree of resistance. Neither catalase enzyme activity nor aquaporins appeared to be involved in H 2 O 2 resistance. Mitochondrial DNA copy number, adenosine triphosphate (ATP) concentration, and plasma membrane potential were decreased. The timing of H 2 O 2 intake was delayed and lipid peroxidation was decreased. Sensitivity of clinically relevant radioresistant cells to H 2 O 2 was enhanced by 1-palmitoyl-2-(5′-oxo-valeroyl)-sn-glycero-3-phosphocholine administration. These results suggest that the membrane status is a major factor conferring H 2 O 2 resistance in clinically relevant radioresistant cells, and we should further investigate how membrane status could be used to enhance the therapeutic effect on cancer. |
| format | Article |
| id | doaj-art-8c9e911fa93743dfb51b1f6c358d8422 |
| institution | Kabale University |
| issn | 1423-0380 |
| language | English |
| publishDate | 2018-09-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Tumor Biology |
| spelling | doaj-art-8c9e911fa93743dfb51b1f6c358d84222025-08-20T03:34:51ZengSAGE PublishingTumor Biology1423-03802018-09-014010.1177/1010428318799250Clinically relevant radioresistant cells exhibit resistance to HO by decreasing internal HO and lipid peroxidationKazuo Tomita0Yoshikazu Kuwahara1Yuko Takashi2Kento Igarashi3Taisuke Nagasawa4Hideki Nabika5Akihiro Kurimasa6Manabu Fukumoto7Yoshihiro Nishitani8Tomoaki Sato9Department of Applied Pharmacology, Graduate School of Medical and Dental Sciences, Kagoshima University, Kagoshima, JapanDepartment of Radiation Biology and Medicine, Faculty of Medicine, Tohoku Medical and Pharmaceutical University, Sendai, JapanDepartment of Restorative Dentistry and Endodontology, Graduate School of Medical and Dental Sciences, Kagoshima University, Kagoshima, JapanDepartment of Applied Pharmacology, Graduate School of Medical and Dental Sciences, Kagoshima University, Kagoshima, JapanDepartment of Applied Pharmacology, Graduate School of Medical and Dental Sciences, Kagoshima University, Kagoshima, JapanDepartment of Material and Biological Chemistry, Faculty of Science, Yamagata University, Yamagata, JapanDepartment of Radiation Biology and Medicine, Faculty of Medicine, Tohoku Medical and Pharmaceutical University, Sendai, JapanDepartment of Molecular Pathology, Tokyo Medical University, Tokyo, JapanDepartment of Restorative Dentistry and Endodontology, Graduate School of Medical and Dental Sciences, Kagoshima University, Kagoshima, JapanDepartment of Applied Pharmacology, Graduate School of Medical and Dental Sciences, Kagoshima University, Kagoshima, JapanRadiation therapy is one of the choices to treat malignant tumors. In radiation therapy, existence of radiation-resistant cell is a major problem to overcome. We established clinically relevant radioresistant cells that had been obtained by exposing to 2 Gy/day X-rays for more than 30 days. These cells are resistant to 2 Gy/day X-ray exposure and anticancer agents. However, the underlying resistance mechanism remains unclear. We investigated the resistance of clinically relevant radioresistant cells to hydrogen peroxide (H 2 O 2 ), confirming a degree of resistance. Neither catalase enzyme activity nor aquaporins appeared to be involved in H 2 O 2 resistance. Mitochondrial DNA copy number, adenosine triphosphate (ATP) concentration, and plasma membrane potential were decreased. The timing of H 2 O 2 intake was delayed and lipid peroxidation was decreased. Sensitivity of clinically relevant radioresistant cells to H 2 O 2 was enhanced by 1-palmitoyl-2-(5′-oxo-valeroyl)-sn-glycero-3-phosphocholine administration. These results suggest that the membrane status is a major factor conferring H 2 O 2 resistance in clinically relevant radioresistant cells, and we should further investigate how membrane status could be used to enhance the therapeutic effect on cancer.https://doi.org/10.1177/1010428318799250 |
| spellingShingle | Kazuo Tomita Yoshikazu Kuwahara Yuko Takashi Kento Igarashi Taisuke Nagasawa Hideki Nabika Akihiro Kurimasa Manabu Fukumoto Yoshihiro Nishitani Tomoaki Sato Clinically relevant radioresistant cells exhibit resistance to HO by decreasing internal HO and lipid peroxidation Tumor Biology |
| title | Clinically relevant radioresistant cells exhibit resistance to HO by decreasing internal HO and lipid peroxidation |
| title_full | Clinically relevant radioresistant cells exhibit resistance to HO by decreasing internal HO and lipid peroxidation |
| title_fullStr | Clinically relevant radioresistant cells exhibit resistance to HO by decreasing internal HO and lipid peroxidation |
| title_full_unstemmed | Clinically relevant radioresistant cells exhibit resistance to HO by decreasing internal HO and lipid peroxidation |
| title_short | Clinically relevant radioresistant cells exhibit resistance to HO by decreasing internal HO and lipid peroxidation |
| title_sort | clinically relevant radioresistant cells exhibit resistance to ho by decreasing internal ho and lipid peroxidation |
| url | https://doi.org/10.1177/1010428318799250 |
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