Downregulation of an Evolutionary Young miR-1290 in an iPSC-Derived Neural Stem Cell Model of Autism Spectrum Disorder
The identification of several evolutionary young miRNAs, which arose in primates, raised several possibilities for the role of such miRNAs in human-specific disease processes. We previously have identified an evolutionary young miRNA, miR-1290, to be essential in neural stem cell proliferation and n...
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| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2019-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2019/8710180 |
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| author | Dalia Moore Brittney M. Meays Lepakshe S. V. Madduri Farah Shahjin Subhash Chand Meng Niu Abrar Albahrani Chittibabu Guda Gurudutt Pendyala Howard S. Fox Sowmya V. Yelamanchili |
| author_facet | Dalia Moore Brittney M. Meays Lepakshe S. V. Madduri Farah Shahjin Subhash Chand Meng Niu Abrar Albahrani Chittibabu Guda Gurudutt Pendyala Howard S. Fox Sowmya V. Yelamanchili |
| author_sort | Dalia Moore |
| collection | DOAJ |
| description | The identification of several evolutionary young miRNAs, which arose in primates, raised several possibilities for the role of such miRNAs in human-specific disease processes. We previously have identified an evolutionary young miRNA, miR-1290, to be essential in neural stem cell proliferation and neuronal differentiation. Here, we show that miR-1290 is significantly downregulated during neuronal differentiation in reprogrammed induced pluripotent stem cell- (iPSC-) derived neurons obtained from idiopathic autism spectrum disorder (ASD) patients. Further, we identified that miR-1290 is actively released into extracellular vesicles. Supplementing ASD patient-derived neural stem cells (NSCs) with conditioned media from differentiated control-NSCs spiked with “artificial EVs” containing synthetic miR-1290 oligonucleotides significantly rescued differentiation deficits in ASD cell lines. Based on our earlier published study and the observations from the data presented here, we conclude that miR-1290 regulation could play a critical role during neuronal differentiation in early brain development. |
| format | Article |
| id | doaj-art-8c6c7279767a41df864a0014cbb5e3af |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-8c6c7279767a41df864a0014cbb5e3af2025-02-03T00:59:05ZengWileyStem Cells International1687-966X1687-96782019-01-01201910.1155/2019/87101808710180Downregulation of an Evolutionary Young miR-1290 in an iPSC-Derived Neural Stem Cell Model of Autism Spectrum DisorderDalia Moore0Brittney M. Meays1Lepakshe S. V. Madduri2Farah Shahjin3Subhash Chand4Meng Niu5Abrar Albahrani6Chittibabu Guda7Gurudutt Pendyala8Howard S. Fox9Sowmya V. Yelamanchili10Department of Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center, Omaha, NE, USAUniversity of Arkansas for Medical Sciences, Little Rock, AR, USADepartment of Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center, Omaha, NE, USADepartment of Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center, Omaha, NE, USADepartment of Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center, Omaha, NE, USADepartment of Genetics Cell Biology and Anatomy, University of Nebraska Medical Center, Omaha, NE, USADepartment of Genetics Cell Biology and Anatomy, University of Nebraska Medical Center, Omaha, NE, USADepartment of Genetics Cell Biology and Anatomy, University of Nebraska Medical Center, Omaha, NE, USADepartment of Anesthesiology, University of Nebraska Medical Center, Omaha, NE, USADepartment of Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center, Omaha, NE, USADepartment of Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center, Omaha, NE, USAThe identification of several evolutionary young miRNAs, which arose in primates, raised several possibilities for the role of such miRNAs in human-specific disease processes. We previously have identified an evolutionary young miRNA, miR-1290, to be essential in neural stem cell proliferation and neuronal differentiation. Here, we show that miR-1290 is significantly downregulated during neuronal differentiation in reprogrammed induced pluripotent stem cell- (iPSC-) derived neurons obtained from idiopathic autism spectrum disorder (ASD) patients. Further, we identified that miR-1290 is actively released into extracellular vesicles. Supplementing ASD patient-derived neural stem cells (NSCs) with conditioned media from differentiated control-NSCs spiked with “artificial EVs” containing synthetic miR-1290 oligonucleotides significantly rescued differentiation deficits in ASD cell lines. Based on our earlier published study and the observations from the data presented here, we conclude that miR-1290 regulation could play a critical role during neuronal differentiation in early brain development.http://dx.doi.org/10.1155/2019/8710180 |
| spellingShingle | Dalia Moore Brittney M. Meays Lepakshe S. V. Madduri Farah Shahjin Subhash Chand Meng Niu Abrar Albahrani Chittibabu Guda Gurudutt Pendyala Howard S. Fox Sowmya V. Yelamanchili Downregulation of an Evolutionary Young miR-1290 in an iPSC-Derived Neural Stem Cell Model of Autism Spectrum Disorder Stem Cells International |
| title | Downregulation of an Evolutionary Young miR-1290 in an iPSC-Derived Neural Stem Cell Model of Autism Spectrum Disorder |
| title_full | Downregulation of an Evolutionary Young miR-1290 in an iPSC-Derived Neural Stem Cell Model of Autism Spectrum Disorder |
| title_fullStr | Downregulation of an Evolutionary Young miR-1290 in an iPSC-Derived Neural Stem Cell Model of Autism Spectrum Disorder |
| title_full_unstemmed | Downregulation of an Evolutionary Young miR-1290 in an iPSC-Derived Neural Stem Cell Model of Autism Spectrum Disorder |
| title_short | Downregulation of an Evolutionary Young miR-1290 in an iPSC-Derived Neural Stem Cell Model of Autism Spectrum Disorder |
| title_sort | downregulation of an evolutionary young mir 1290 in an ipsc derived neural stem cell model of autism spectrum disorder |
| url | http://dx.doi.org/10.1155/2019/8710180 |
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