PCR-Based Detection and Genotyping of Helicobacter pylori in Endoscopic Biopsy Samples from Brazilian Patients
Helicobacter pylori (H. pylori) is considered the second most prevalent infection in man. A precise diagnosis is important for treating patients with the indicative gastrointestinal symptoms. The present study analyzes the effectiveness of a molecular biology me...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | Gastroenterology Research and Practice |
| Online Access: | http://dx.doi.org/10.1155/2013/951034 |
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| author | Silvia M. Ferreira Menoni Sandra Helena Alves Bonon José Murilo Robilota Zeitune Sandra Cecília Botelho Costa |
| author_facet | Silvia M. Ferreira Menoni Sandra Helena Alves Bonon José Murilo Robilota Zeitune Sandra Cecília Botelho Costa |
| author_sort | Silvia M. Ferreira Menoni |
| collection | DOAJ |
| description | Helicobacter pylori (H. pylori) is considered the second most
prevalent infection in man. A precise diagnosis is important for treating patients with the indicative
gastrointestinal symptoms. The present study analyzes the effectiveness of a molecular biology method
(PCR) comparing the results obtained with the histology and with the rapid urease tests.
PCR was used in the detection and genotyping of the H. pylori urease-C gene and the patterns which were obtained from the patients studied. 141 biopsy samples from 131 patients were evaluated. 59 paraffin biopsies samples were positive for H. pylori according to the histological examination. Of those, 59/12 (20.3%) were amplified using PCR. Of the 82 samples from the fresh biopsies, 64 were positive for H. pylori according to the rapid urease test (78%); there was an agreement of 100% with PCR. Sixty positive H. pylori samples were genotyped (58 samples of fresh biopsies and 2 samples of paraffin biopsies) using two restriction enzymes. The patterns observed were analyzed with the computational program BIO 1D; 11 patterns with the enzyme HhaI and 12 patterns with the enzyme MboI were found. However, it was not possible to find a statistically significant correlation between the specific genotypes and digestive pathologies. Accordingly, future research should be performed to confirm a statistically significant relationship between genotyping and gastrointestinal symptoms. |
| format | Article |
| id | doaj-art-8c6a00e20d2546a0ab4ce033b73f4d4e |
| institution | Kabale University |
| issn | 1687-6121 1687-630X |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Gastroenterology Research and Practice |
| spelling | doaj-art-8c6a00e20d2546a0ab4ce033b73f4d4e2025-02-03T06:08:25ZengWileyGastroenterology Research and Practice1687-61211687-630X2013-01-01201310.1155/2013/951034951034PCR-Based Detection and Genotyping of Helicobacter pylori in Endoscopic Biopsy Samples from Brazilian PatientsSilvia M. Ferreira Menoni0Sandra Helena Alves Bonon1José Murilo Robilota Zeitune2Sandra Cecília Botelho Costa3Department of Internal Medicine, Faculty of Medical Sciences, State University of Campinas (UNICAMP), 13081-970 Campinas, SP, BrazilDepartment of Internal Medicine, Faculty of Medical Sciences, State University of Campinas (UNICAMP), 13081-970 Campinas, SP, BrazilGastrocentro, State University of Campinas (UNICAMP), 13083-878 Campinas, SP, BrazilDepartment of Internal Medicine, Faculty of Medical Sciences, State University of Campinas (UNICAMP), 13081-970 Campinas, SP, BrazilHelicobacter pylori (H. pylori) is considered the second most prevalent infection in man. A precise diagnosis is important for treating patients with the indicative gastrointestinal symptoms. The present study analyzes the effectiveness of a molecular biology method (PCR) comparing the results obtained with the histology and with the rapid urease tests. PCR was used in the detection and genotyping of the H. pylori urease-C gene and the patterns which were obtained from the patients studied. 141 biopsy samples from 131 patients were evaluated. 59 paraffin biopsies samples were positive for H. pylori according to the histological examination. Of those, 59/12 (20.3%) were amplified using PCR. Of the 82 samples from the fresh biopsies, 64 were positive for H. pylori according to the rapid urease test (78%); there was an agreement of 100% with PCR. Sixty positive H. pylori samples were genotyped (58 samples of fresh biopsies and 2 samples of paraffin biopsies) using two restriction enzymes. The patterns observed were analyzed with the computational program BIO 1D; 11 patterns with the enzyme HhaI and 12 patterns with the enzyme MboI were found. However, it was not possible to find a statistically significant correlation between the specific genotypes and digestive pathologies. Accordingly, future research should be performed to confirm a statistically significant relationship between genotyping and gastrointestinal symptoms.http://dx.doi.org/10.1155/2013/951034 |
| spellingShingle | Silvia M. Ferreira Menoni Sandra Helena Alves Bonon José Murilo Robilota Zeitune Sandra Cecília Botelho Costa PCR-Based Detection and Genotyping of Helicobacter pylori in Endoscopic Biopsy Samples from Brazilian Patients Gastroenterology Research and Practice |
| title | PCR-Based Detection and Genotyping of Helicobacter pylori in Endoscopic Biopsy Samples from Brazilian Patients |
| title_full | PCR-Based Detection and Genotyping of Helicobacter pylori in Endoscopic Biopsy Samples from Brazilian Patients |
| title_fullStr | PCR-Based Detection and Genotyping of Helicobacter pylori in Endoscopic Biopsy Samples from Brazilian Patients |
| title_full_unstemmed | PCR-Based Detection and Genotyping of Helicobacter pylori in Endoscopic Biopsy Samples from Brazilian Patients |
| title_short | PCR-Based Detection and Genotyping of Helicobacter pylori in Endoscopic Biopsy Samples from Brazilian Patients |
| title_sort | pcr based detection and genotyping of helicobacter pylori in endoscopic biopsy samples from brazilian patients |
| url | http://dx.doi.org/10.1155/2013/951034 |
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