Layer-by-Layer Assembly of Polysaccharides and 6,10-Ionene for Separation of Nitrogen-Containing Pharmaceuticals and Their Enantiorecognition by Capillary Electrophoresis
Two silica capillaries modified layer-by-layer with 6,10-ionene and N-(3-sulfo-3-carboxy)-propionylchitosan (SCPC) and with 6,10-ionene and dextran sulfate (DS) were prepared and investigated. Dynamic coating of the capillary efficiently reduces the adsorption of the background electrolyte, sample m...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | Journal of Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2015/836076 |
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| author | Anna Ioutsi Elena Shapovalova Aleksandra Prokhorova Oleg Shpigun |
| author_facet | Anna Ioutsi Elena Shapovalova Aleksandra Prokhorova Oleg Shpigun |
| author_sort | Anna Ioutsi |
| collection | DOAJ |
| description | Two silica capillaries modified layer-by-layer with 6,10-ionene and N-(3-sulfo-3-carboxy)-propionylchitosan (SCPC) and with 6,10-ionene and dextran sulfate (DS) were prepared and investigated. Dynamic coating of the capillary efficiently reduces the adsorption of the background electrolyte, sample matrix components, and analytes on its inner wall. Such coatings effect good reproducibility and sensitivity of determination. We demonstrate that separation of betablockers, calcium channel blockers, alpha-adrenergic agonists, H1-blockers, and diuretics was the most efficient and rapid separation with a capillary modified with dextran sulfate. Tetrahydrozoline, carbinoxamine, and furacilin, which are commonly employed as treatments for allergic rhinitis, were identified in human urea. Their concentrations, independently verified by HPLC, were found to be 5.3±0.8, 6.6±0.5, and 0.9±0.2 μg mL−1, with LOD=0.07, 0.03, 0.10 μg mL−1, and LOQ=1.0, 0.8, 0.6 μg mL−1, respectively. |
| format | Article |
| id | doaj-art-8be2a64bd6fd49bb9eeb194e6ea7b18c |
| institution | Kabale University |
| issn | 2090-9063 2090-9071 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Chemistry |
| spelling | doaj-art-8be2a64bd6fd49bb9eeb194e6ea7b18c2025-02-03T01:12:30ZengWileyJournal of Chemistry2090-90632090-90712015-01-01201510.1155/2015/836076836076Layer-by-Layer Assembly of Polysaccharides and 6,10-Ionene for Separation of Nitrogen-Containing Pharmaceuticals and Their Enantiorecognition by Capillary ElectrophoresisAnna Ioutsi0Elena Shapovalova1Aleksandra Prokhorova2Oleg Shpigun3Analytical Chemistry Division, Department of Chemistry, Lomonosov Moscow State University, Leninskie Gory 1/3, Moscow 119991, RussiaAnalytical Chemistry Division, Department of Chemistry, Lomonosov Moscow State University, Leninskie Gory 1/3, Moscow 119991, RussiaAnalytical Chemistry Division, Department of Chemistry, Lomonosov Moscow State University, Leninskie Gory 1/3, Moscow 119991, RussiaAnalytical Chemistry Division, Department of Chemistry, Lomonosov Moscow State University, Leninskie Gory 1/3, Moscow 119991, RussiaTwo silica capillaries modified layer-by-layer with 6,10-ionene and N-(3-sulfo-3-carboxy)-propionylchitosan (SCPC) and with 6,10-ionene and dextran sulfate (DS) were prepared and investigated. Dynamic coating of the capillary efficiently reduces the adsorption of the background electrolyte, sample matrix components, and analytes on its inner wall. Such coatings effect good reproducibility and sensitivity of determination. We demonstrate that separation of betablockers, calcium channel blockers, alpha-adrenergic agonists, H1-blockers, and diuretics was the most efficient and rapid separation with a capillary modified with dextran sulfate. Tetrahydrozoline, carbinoxamine, and furacilin, which are commonly employed as treatments for allergic rhinitis, were identified in human urea. Their concentrations, independently verified by HPLC, were found to be 5.3±0.8, 6.6±0.5, and 0.9±0.2 μg mL−1, with LOD=0.07, 0.03, 0.10 μg mL−1, and LOQ=1.0, 0.8, 0.6 μg mL−1, respectively.http://dx.doi.org/10.1155/2015/836076 |
| spellingShingle | Anna Ioutsi Elena Shapovalova Aleksandra Prokhorova Oleg Shpigun Layer-by-Layer Assembly of Polysaccharides and 6,10-Ionene for Separation of Nitrogen-Containing Pharmaceuticals and Their Enantiorecognition by Capillary Electrophoresis Journal of Chemistry |
| title | Layer-by-Layer Assembly of Polysaccharides and 6,10-Ionene for Separation of Nitrogen-Containing Pharmaceuticals and Their Enantiorecognition by Capillary Electrophoresis |
| title_full | Layer-by-Layer Assembly of Polysaccharides and 6,10-Ionene for Separation of Nitrogen-Containing Pharmaceuticals and Their Enantiorecognition by Capillary Electrophoresis |
| title_fullStr | Layer-by-Layer Assembly of Polysaccharides and 6,10-Ionene for Separation of Nitrogen-Containing Pharmaceuticals and Their Enantiorecognition by Capillary Electrophoresis |
| title_full_unstemmed | Layer-by-Layer Assembly of Polysaccharides and 6,10-Ionene for Separation of Nitrogen-Containing Pharmaceuticals and Their Enantiorecognition by Capillary Electrophoresis |
| title_short | Layer-by-Layer Assembly of Polysaccharides and 6,10-Ionene for Separation of Nitrogen-Containing Pharmaceuticals and Their Enantiorecognition by Capillary Electrophoresis |
| title_sort | layer by layer assembly of polysaccharides and 6 10 ionene for separation of nitrogen containing pharmaceuticals and their enantiorecognition by capillary electrophoresis |
| url | http://dx.doi.org/10.1155/2015/836076 |
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