Molecular Identification and Traceability of Illegal Trading in Lignobrycon myersi (Teleostei: Characiformes), a Threatened Brazilian Fish Species, Using DNA Barcode
Lignobrycon myersi is a threatened freshwater fish species and endemic of a few coastal rivers in northeastern Brazil. Even though the Brazilian laws prohibit the fisheries of threatened species, L. myersi is occasionally found in street markets, being highly appreciated by local population. In orde...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2016/9382613 |
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| author | Alexandre dos Santos Rodrigues José Henrique Souza Galdino Brandão Jamille de Araújo Bitencourt Ricardo Jucá-Chagas Iracilda Sampaio Horácio Schneider Paulo Roberto Antunes de Mello Affonso |
| author_facet | Alexandre dos Santos Rodrigues José Henrique Souza Galdino Brandão Jamille de Araújo Bitencourt Ricardo Jucá-Chagas Iracilda Sampaio Horácio Schneider Paulo Roberto Antunes de Mello Affonso |
| author_sort | Alexandre dos Santos Rodrigues |
| collection | DOAJ |
| description | Lignobrycon myersi is a threatened freshwater fish species and endemic of a few coastal rivers in northeastern Brazil. Even though the Brazilian laws prohibit the fisheries of threatened species, L. myersi is occasionally found in street markets, being highly appreciated by local population. In order to provide a reliable DNA barcode dataset for L. myersi, we compared mitochondrial sequences of cytochrome c oxidase subunit I (COI) from fresh, frozen, and salt-preserved specimens. Phylogenetically related species (Triportheus spp.) and other fish species (Astyanax fasciatus) commonly mixed with L. myersi in street markets were also included to test the efficiency of molecular identification. In spite of the differences in conservation processes and advanced deterioration of some commercial samples, high-quality COI sequences were obtained and effective in discriminating L. myersi specimens. In addition, while populations from Contas and Almada River basins seem to comprise a single evolutionary lineage, the specimens from Cachoeira River were genetically differentiated, indicating population structuring. Therefore, DNA barcoding has proved to be useful to trace the illegal trading of L. myersi and to manage threatened populations, which should focus on conservation of distinct genetic stocks and mitigation on human impacts along their range. |
| format | Article |
| id | doaj-art-8b64558ae8eb4a0b99dea1ebd409c9b9 |
| institution | Kabale University |
| issn | 2356-6140 1537-744X |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
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| series | The Scientific World Journal |
| spelling | doaj-art-8b64558ae8eb4a0b99dea1ebd409c9b92025-02-03T05:57:21ZengWileyThe Scientific World Journal2356-61401537-744X2016-01-01201610.1155/2016/93826139382613Molecular Identification and Traceability of Illegal Trading in Lignobrycon myersi (Teleostei: Characiformes), a Threatened Brazilian Fish Species, Using DNA BarcodeAlexandre dos Santos Rodrigues0José Henrique Souza Galdino Brandão1Jamille de Araújo Bitencourt2Ricardo Jucá-Chagas3Iracilda Sampaio4Horácio Schneider5Paulo Roberto Antunes de Mello Affonso6Department of Biological Sciences, Universidade Estadual do Sudoeste da Bahia, Rua José Moreira Sobrinho, s/n, Bairro Jequiezinho, 45206-190 Jequié, BA, BrazilDepartment of Biological Sciences, Universidade Estadual do Sudoeste da Bahia, Rua José Moreira Sobrinho, s/n, Bairro Jequiezinho, 45206-190 Jequié, BA, BrazilDepartment of Biological Sciences, Universidade Estadual do Sudoeste da Bahia, Rua José Moreira Sobrinho, s/n, Bairro Jequiezinho, 45206-190 Jequié, BA, BrazilDepartment of Biological Sciences, Universidade Estadual do Sudoeste da Bahia, Rua José Moreira Sobrinho, s/n, Bairro Jequiezinho, 45206-190 Jequié, BA, BrazilInstitute of Coastal Studies (IECOS), Universidade Federal do Pará, Alameda Leandro Ribeiro s/n, Bairro Aldeia, 68600-000 Bragança, PA, BrazilInstitute of Coastal Studies (IECOS), Universidade Federal do Pará, Alameda Leandro Ribeiro s/n, Bairro Aldeia, 68600-000 Bragança, PA, BrazilDepartment of Biological Sciences, Universidade Estadual do Sudoeste da Bahia, Rua José Moreira Sobrinho, s/n, Bairro Jequiezinho, 45206-190 Jequié, BA, BrazilLignobrycon myersi is a threatened freshwater fish species and endemic of a few coastal rivers in northeastern Brazil. Even though the Brazilian laws prohibit the fisheries of threatened species, L. myersi is occasionally found in street markets, being highly appreciated by local population. In order to provide a reliable DNA barcode dataset for L. myersi, we compared mitochondrial sequences of cytochrome c oxidase subunit I (COI) from fresh, frozen, and salt-preserved specimens. Phylogenetically related species (Triportheus spp.) and other fish species (Astyanax fasciatus) commonly mixed with L. myersi in street markets were also included to test the efficiency of molecular identification. In spite of the differences in conservation processes and advanced deterioration of some commercial samples, high-quality COI sequences were obtained and effective in discriminating L. myersi specimens. In addition, while populations from Contas and Almada River basins seem to comprise a single evolutionary lineage, the specimens from Cachoeira River were genetically differentiated, indicating population structuring. Therefore, DNA barcoding has proved to be useful to trace the illegal trading of L. myersi and to manage threatened populations, which should focus on conservation of distinct genetic stocks and mitigation on human impacts along their range.http://dx.doi.org/10.1155/2016/9382613 |
| spellingShingle | Alexandre dos Santos Rodrigues José Henrique Souza Galdino Brandão Jamille de Araújo Bitencourt Ricardo Jucá-Chagas Iracilda Sampaio Horácio Schneider Paulo Roberto Antunes de Mello Affonso Molecular Identification and Traceability of Illegal Trading in Lignobrycon myersi (Teleostei: Characiformes), a Threatened Brazilian Fish Species, Using DNA Barcode The Scientific World Journal |
| title | Molecular Identification and Traceability of Illegal Trading in Lignobrycon myersi (Teleostei: Characiformes), a Threatened Brazilian Fish Species, Using DNA Barcode |
| title_full | Molecular Identification and Traceability of Illegal Trading in Lignobrycon myersi (Teleostei: Characiformes), a Threatened Brazilian Fish Species, Using DNA Barcode |
| title_fullStr | Molecular Identification and Traceability of Illegal Trading in Lignobrycon myersi (Teleostei: Characiformes), a Threatened Brazilian Fish Species, Using DNA Barcode |
| title_full_unstemmed | Molecular Identification and Traceability of Illegal Trading in Lignobrycon myersi (Teleostei: Characiformes), a Threatened Brazilian Fish Species, Using DNA Barcode |
| title_short | Molecular Identification and Traceability of Illegal Trading in Lignobrycon myersi (Teleostei: Characiformes), a Threatened Brazilian Fish Species, Using DNA Barcode |
| title_sort | molecular identification and traceability of illegal trading in lignobrycon myersi teleostei characiformes a threatened brazilian fish species using dna barcode |
| url | http://dx.doi.org/10.1155/2016/9382613 |
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