Autologous induced pluripotent stem cell-derived four-organ-chip
Microphysiological systems play a pivotal role in progressing toward a global paradigm shift in drug development. Here, we designed a four-organ-chip interconnecting miniaturized human intestine, liver, brain and kidney equivalents. All four organ models were predifferentiated from induced pluripote...
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| Main Authors: | , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2019-09-01
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| Series: | Future Science OA |
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| Online Access: | https://www.future-science.com/doi/10.2144/fsoa-2019-0065 |
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| author | Anja Patricia Ramme Leopold Koenig Tobias Hasenberg Christine Schwenk Corinna Magauer Daniel Faust Alexandra K Lorenz Anna-Catharina Krebs Christopher Drewell Kerstin Schirrmann Alexandra Vladetic Grace-Chiaen Lin Stephan Pabinger Winfried Neuhaus Frederic Bois Roland Lauster Uwe Marx Eva-Maria Dehne |
| author_facet | Anja Patricia Ramme Leopold Koenig Tobias Hasenberg Christine Schwenk Corinna Magauer Daniel Faust Alexandra K Lorenz Anna-Catharina Krebs Christopher Drewell Kerstin Schirrmann Alexandra Vladetic Grace-Chiaen Lin Stephan Pabinger Winfried Neuhaus Frederic Bois Roland Lauster Uwe Marx Eva-Maria Dehne |
| author_sort | Anja Patricia Ramme |
| collection | DOAJ |
| description | Microphysiological systems play a pivotal role in progressing toward a global paradigm shift in drug development. Here, we designed a four-organ-chip interconnecting miniaturized human intestine, liver, brain and kidney equivalents. All four organ models were predifferentiated from induced pluripotent stem cells from the same healthy donor and integrated into the microphysiological system. The coculture of the four autologous tissue models in one common medium deprived of tissue specific growth factors was successful over 14-days. Although there were no added growth factors present in the coculture medium, the intestine, liver and neuronal model maintained defined marker expression. Only the renal model was overgrown by coexisting cells and did not further differentiate. This model platform will pave the way for autologous coculture cross-talk assays, disease induction and subsequent drug testing. |
| format | Article |
| id | doaj-art-8b4907e94de7419ea2fc0f71c05acb8f |
| institution | OA Journals |
| issn | 2056-5623 |
| language | English |
| publishDate | 2019-09-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Future Science OA |
| spelling | doaj-art-8b4907e94de7419ea2fc0f71c05acb8f2025-08-20T02:25:50ZengTaylor & Francis GroupFuture Science OA2056-56232019-09-015810.2144/fsoa-2019-0065Autologous induced pluripotent stem cell-derived four-organ-chipAnja Patricia Ramme0Leopold Koenig1Tobias Hasenberg2Christine Schwenk3Corinna Magauer4Daniel Faust5Alexandra K Lorenz6Anna-Catharina Krebs7Christopher Drewell8Kerstin Schirrmann9Alexandra Vladetic10Grace-Chiaen Lin11Stephan Pabinger12Winfried Neuhaus13Frederic Bois14Roland Lauster15Uwe Marx16Eva-Maria Dehne171TissUse GmbH, Oudenarder Str. 16, 13347 Berlin, Deutschland1TissUse GmbH, Oudenarder Str. 16, 13347 Berlin, Deutschland1TissUse GmbH, Oudenarder Str. 16, 13347 Berlin, Deutschland1TissUse GmbH, Oudenarder Str. 16, 13347 Berlin, Deutschland1TissUse GmbH, Oudenarder Str. 16, 13347 Berlin, Deutschland1TissUse GmbH, Oudenarder Str. 16, 13347 Berlin, Deutschland1TissUse GmbH, Oudenarder Str. 16, 13347 Berlin, Deutschland1TissUse GmbH, Oudenarder Str. 16, 13347 Berlin, Deutschland2Technische Universität Berlin, Medizinische Biotechnologie, Gustav-Meyer-Allee 25, 13355 Berlin, Deutschland3The University of Manchester, Physics of Fluids & Soft Matter Group, Oxford Road, Manchester M13 9PL, UK4AIT Austrian Institute of Technology GmbH, Giefinggasse 4, 1210 Vienna, Austria4AIT Austrian Institute of Technology GmbH, Giefinggasse 4, 1210 Vienna, Austria4AIT Austrian Institute of Technology GmbH, Giefinggasse 4, 1210 Vienna, Austria4AIT Austrian Institute of Technology GmbH, Giefinggasse 4, 1210 Vienna, Austria5INERIS, METO unit, Parc ALATA BP2, 60550 Verneuil en Halatte, France2Technische Universität Berlin, Medizinische Biotechnologie, Gustav-Meyer-Allee 25, 13355 Berlin, Deutschland1TissUse GmbH, Oudenarder Str. 16, 13347 Berlin, Deutschland1TissUse GmbH, Oudenarder Str. 16, 13347 Berlin, DeutschlandMicrophysiological systems play a pivotal role in progressing toward a global paradigm shift in drug development. Here, we designed a four-organ-chip interconnecting miniaturized human intestine, liver, brain and kidney equivalents. All four organ models were predifferentiated from induced pluripotent stem cells from the same healthy donor and integrated into the microphysiological system. The coculture of the four autologous tissue models in one common medium deprived of tissue specific growth factors was successful over 14-days. Although there were no added growth factors present in the coculture medium, the intestine, liver and neuronal model maintained defined marker expression. Only the renal model was overgrown by coexisting cells and did not further differentiate. This model platform will pave the way for autologous coculture cross-talk assays, disease induction and subsequent drug testing.https://www.future-science.com/doi/10.2144/fsoa-2019-0065differentiationfour-organ-chipinduced pluripotent stem cellsmicrophysiological systemmulti-organ-chip |
| spellingShingle | Anja Patricia Ramme Leopold Koenig Tobias Hasenberg Christine Schwenk Corinna Magauer Daniel Faust Alexandra K Lorenz Anna-Catharina Krebs Christopher Drewell Kerstin Schirrmann Alexandra Vladetic Grace-Chiaen Lin Stephan Pabinger Winfried Neuhaus Frederic Bois Roland Lauster Uwe Marx Eva-Maria Dehne Autologous induced pluripotent stem cell-derived four-organ-chip Future Science OA differentiation four-organ-chip induced pluripotent stem cells microphysiological system multi-organ-chip |
| title | Autologous induced pluripotent stem cell-derived four-organ-chip |
| title_full | Autologous induced pluripotent stem cell-derived four-organ-chip |
| title_fullStr | Autologous induced pluripotent stem cell-derived four-organ-chip |
| title_full_unstemmed | Autologous induced pluripotent stem cell-derived four-organ-chip |
| title_short | Autologous induced pluripotent stem cell-derived four-organ-chip |
| title_sort | autologous induced pluripotent stem cell derived four organ chip |
| topic | differentiation four-organ-chip induced pluripotent stem cells microphysiological system multi-organ-chip |
| url | https://www.future-science.com/doi/10.2144/fsoa-2019-0065 |
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