pH dependence of the protein orientation in self-assembled bacteriorhodopsin/polycation multilayer films
Bacteriorhodopsin (bR) multilayer films were successfully prepared by alternatingdepositions of cationic poly(diallyldimethylammonium chloride) (PDAC) polyelectrolyte and purple membrane (PM). The advantage of the method when compared to other thin film techniques is its simplicity to prepare large...
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| Format: | Article |
| Language: | English |
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Wiley
2000-01-01
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| Series: | International Journal of Photoenergy |
| Online Access: | http://dx.doi.org/10.1155/S1110662X00000064 |
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| author | Tapio Jussila Meiling Li Nikolai V. Tkachenko Sinikka Parkkinen Baofang Li Long Jiang Helge Lemmetyinen |
| author_facet | Tapio Jussila Meiling Li Nikolai V. Tkachenko Sinikka Parkkinen Baofang Li Long Jiang Helge Lemmetyinen |
| author_sort | Tapio Jussila |
| collection | DOAJ |
| description | Bacteriorhodopsin (bR) multilayer films were successfully prepared by alternatingdepositions
of cationic poly(diallyldimethylammonium chloride) (PDAC) polyelectrolyte and purple membrane (PM). The
advantage of the method when compared to other thin film techniques is its simplicity to prepare large selfassembled
films. The orientations of the bR molecules in the films were studied by a transient photovoltage
method. The polarity of the photoresponse signals showed that in basic conditions the cytoplasmic side of PM
was adsorbed onto the polyelectrolyte layer, whereas in acidic conditions the extracellular side was adsorbed
onto the polyelectrolyte layer. The photocycle of the bR in the films was studied by usingthe conventional
flash photolysis method. The photochemical behavior of bR in dry polycationic films was similar to that in
other types of dry films and the intermediate lifetimes were almost independent of the protein orientation. |
| format | Article |
| id | doaj-art-8ad0f98583424e1f9a9d134d00339908 |
| institution | Kabale University |
| issn | 1110-662X |
| language | English |
| publishDate | 2000-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Photoenergy |
| spelling | doaj-art-8ad0f98583424e1f9a9d134d003399082025-02-03T01:21:10ZengWileyInternational Journal of Photoenergy1110-662X2000-01-0121414510.1155/S1110662X00000064pH dependence of the protein orientation in self-assembled bacteriorhodopsin/polycation multilayer filmsTapio Jussila0Meiling Li1Nikolai V. Tkachenko2Sinikka Parkkinen3Baofang Li4Long Jiang5Helge Lemmetyinen6Institute of Materials Chemistry, Tampere University of Technology, P.O. Box 541, Tampere 33101, FinlandLaboratory of Colloid and Interface Science, Center for Molecular Science, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100101, ChinaInstitute of Materials Chemistry, Tampere University of Technology, P.O. Box 541, Tampere 33101, FinlandUniversity of Joensuu, Department of Biology, P.O. Box 111, 80101, FinlandLaboratory of Colloid and Interface Science, Center for Molecular Science, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100101, ChinaLaboratory of Colloid and Interface Science, Center for Molecular Science, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100101, ChinaInstitute of Materials Chemistry, Tampere University of Technology, P.O. Box 541, Tampere 33101, FinlandBacteriorhodopsin (bR) multilayer films were successfully prepared by alternatingdepositions of cationic poly(diallyldimethylammonium chloride) (PDAC) polyelectrolyte and purple membrane (PM). The advantage of the method when compared to other thin film techniques is its simplicity to prepare large selfassembled films. The orientations of the bR molecules in the films were studied by a transient photovoltage method. The polarity of the photoresponse signals showed that in basic conditions the cytoplasmic side of PM was adsorbed onto the polyelectrolyte layer, whereas in acidic conditions the extracellular side was adsorbed onto the polyelectrolyte layer. The photocycle of the bR in the films was studied by usingthe conventional flash photolysis method. The photochemical behavior of bR in dry polycationic films was similar to that in other types of dry films and the intermediate lifetimes were almost independent of the protein orientation.http://dx.doi.org/10.1155/S1110662X00000064 |
| spellingShingle | Tapio Jussila Meiling Li Nikolai V. Tkachenko Sinikka Parkkinen Baofang Li Long Jiang Helge Lemmetyinen pH dependence of the protein orientation in self-assembled bacteriorhodopsin/polycation multilayer films International Journal of Photoenergy |
| title | pH dependence of the protein orientation in self-assembled bacteriorhodopsin/polycation multilayer films |
| title_full | pH dependence of the protein orientation in self-assembled bacteriorhodopsin/polycation multilayer films |
| title_fullStr | pH dependence of the protein orientation in self-assembled bacteriorhodopsin/polycation multilayer films |
| title_full_unstemmed | pH dependence of the protein orientation in self-assembled bacteriorhodopsin/polycation multilayer films |
| title_short | pH dependence of the protein orientation in self-assembled bacteriorhodopsin/polycation multilayer films |
| title_sort | ph dependence of the protein orientation in self assembled bacteriorhodopsin polycation multilayer films |
| url | http://dx.doi.org/10.1155/S1110662X00000064 |
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