Multiscreening LC-MS/MS Designed for Ten Pesticide and Six Antimicrobial Residues in Eggs
A multiscreening method was experimentally validated for pesticide and antimicrobial residues in spiked eggs at levels of 5, 50, and 500 μg L−1. The residue extraction was performed with acetonitrile and 1% formic acid. The extracted material was stored at −70°C until detection and quantitation with...
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | Journal of Food Quality |
| Online Access: | http://dx.doi.org/10.1155/2017/9718451 |
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| author | Julia Arantes Galvão Ricardo Seiti Yamatogi Alexander Welker Biondo José Paes de Almeida Nogueira Pinto José Roberto Marques Silva Caio Antonio Carbonari Edivaldo Domingues Velini |
| author_facet | Julia Arantes Galvão Ricardo Seiti Yamatogi Alexander Welker Biondo José Paes de Almeida Nogueira Pinto José Roberto Marques Silva Caio Antonio Carbonari Edivaldo Domingues Velini |
| author_sort | Julia Arantes Galvão |
| collection | DOAJ |
| description | A multiscreening method was experimentally validated for pesticide and antimicrobial residues in spiked eggs at levels of 5, 50, and 500 μg L−1. The residue extraction was performed with acetonitrile and 1% formic acid. The extracted material was stored at −70°C until detection and quantitation within 24-hour period by LC-MS/MS. Pesticide and antimicrobial residue recovery performed adequate standard deviation and the results were from 94.10% (ampa) to 9.20% (carbaryl) for pesticides and from 95.70% (enrofloxacin) to 46.20% (sulfathiazole) for antimicrobial. The method was highly accurate, considering the linearity for all pesticide and antimicrobial residues (r2>0.99), with quantitation limits ranging from 0.78 to 6.25 μg L−1 for pesticides and 0.78 to 3.13 μg L−1 for antibiotics. A highly correlation coefficient (r>0.99) was found, except for carbaryl. Although time extraction and carbaryl recovery remain to be improved, the method has shown a rapid pesticide and antimicrobial residue detection. |
| format | Article |
| id | doaj-art-8aa0a24bcdbf46b09e466d4e8e598cfc |
| institution | OA Journals |
| issn | 0146-9428 1745-4557 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Food Quality |
| spelling | doaj-art-8aa0a24bcdbf46b09e466d4e8e598cfc2025-08-20T02:09:25ZengWileyJournal of Food Quality0146-94281745-45572017-01-01201710.1155/2017/97184519718451Multiscreening LC-MS/MS Designed for Ten Pesticide and Six Antimicrobial Residues in EggsJulia Arantes Galvão0Ricardo Seiti Yamatogi1Alexander Welker Biondo2José Paes de Almeida Nogueira Pinto3José Roberto Marques Silva4Caio Antonio Carbonari5Edivaldo Domingues Velini6Department of Veterinary Hygiene and Public Health, School of Veterinary Medicine and Animal Science, Univ. Estadual Paulista (UNESP), Distrito de Rubião Júnior, s/n, 18618-970 Botucatu, SP, BrazilDepartment of Veterinary Hygiene and Public Health, School of Veterinary Medicine and Animal Science, Univ. Estadual Paulista (UNESP), Distrito de Rubião Júnior, s/n, 18618-970 Botucatu, SP, BrazilDepartment of Veterinary Medicine, Federal University of Parana, 80035-050 Curitiba, PR, BrazilDepartment of Veterinary Hygiene and Public Health, School of Veterinary Medicine and Animal Science, Univ. Estadual Paulista (UNESP), Distrito de Rubião Júnior, s/n, 18618-970 Botucatu, SP, BrazilCenter for Advanced Research in Matology (CARM), Chromatography Laboratory, Faculty of Agricultural Sciences, Paulista State University, 18618-970 Botucatu, SP, BrazilCenter for Advanced Research in Matology (CARM), Chromatography Laboratory, Faculty of Agricultural Sciences, Paulista State University, 18618-970 Botucatu, SP, BrazilCenter for Advanced Research in Matology (CARM), Chromatography Laboratory, Faculty of Agricultural Sciences, Paulista State University, 18618-970 Botucatu, SP, BrazilA multiscreening method was experimentally validated for pesticide and antimicrobial residues in spiked eggs at levels of 5, 50, and 500 μg L−1. The residue extraction was performed with acetonitrile and 1% formic acid. The extracted material was stored at −70°C until detection and quantitation within 24-hour period by LC-MS/MS. Pesticide and antimicrobial residue recovery performed adequate standard deviation and the results were from 94.10% (ampa) to 9.20% (carbaryl) for pesticides and from 95.70% (enrofloxacin) to 46.20% (sulfathiazole) for antimicrobial. The method was highly accurate, considering the linearity for all pesticide and antimicrobial residues (r2>0.99), with quantitation limits ranging from 0.78 to 6.25 μg L−1 for pesticides and 0.78 to 3.13 μg L−1 for antibiotics. A highly correlation coefficient (r>0.99) was found, except for carbaryl. Although time extraction and carbaryl recovery remain to be improved, the method has shown a rapid pesticide and antimicrobial residue detection.http://dx.doi.org/10.1155/2017/9718451 |
| spellingShingle | Julia Arantes Galvão Ricardo Seiti Yamatogi Alexander Welker Biondo José Paes de Almeida Nogueira Pinto José Roberto Marques Silva Caio Antonio Carbonari Edivaldo Domingues Velini Multiscreening LC-MS/MS Designed for Ten Pesticide and Six Antimicrobial Residues in Eggs Journal of Food Quality |
| title | Multiscreening LC-MS/MS Designed for Ten Pesticide and Six Antimicrobial Residues in Eggs |
| title_full | Multiscreening LC-MS/MS Designed for Ten Pesticide and Six Antimicrobial Residues in Eggs |
| title_fullStr | Multiscreening LC-MS/MS Designed for Ten Pesticide and Six Antimicrobial Residues in Eggs |
| title_full_unstemmed | Multiscreening LC-MS/MS Designed for Ten Pesticide and Six Antimicrobial Residues in Eggs |
| title_short | Multiscreening LC-MS/MS Designed for Ten Pesticide and Six Antimicrobial Residues in Eggs |
| title_sort | multiscreening lc ms ms designed for ten pesticide and six antimicrobial residues in eggs |
| url | http://dx.doi.org/10.1155/2017/9718451 |
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