COMBO-FISH: specific labeling of nondenatured chromatin targets by computer-selected DNA oligonucleotide probe combinations

Here we present the principle of fluorescence in situ hybridization (FISH) with combinatorial oligonucleotide (COMBO) probes as a new approach for the specific labeling of genomic sites. COMBO-FISH takes advantage of homopurine/homopyrimidine oligonucleotides that form triple helices with intact dup...

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Bibliographic Details
Main Authors: Michael Hausmann, Ralph Winkler, Georg Hildenbrand, Jutta Finsterle, Andrea Weisel, Alexander Rapp, Eberhard Schmitt, Siegfried Janz, Christoph Cremer
Format: Article
Language:English
Published: Taylor & Francis Group 2003-09-01
Series:BioTechniques
Online Access:https://www.future-science.com/doi/10.2144/03353rr03
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