Photo‐inactivation of bacteria in hospital effluent via thiolated iron‐doped nanoceria
Hospital wastewater is a major contributor of disease‐causing microbes and the emergence of antibiotic resistant bacteria. In this study, thiolated iron‐doped nanoceria was synthesised and tested for killing of microbes from hospital effluent. These particles were designed to inhibit the efflux pump...
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| Format: | Article |
| Language: | English |
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Wiley
2019-10-01
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| Series: | IET Nanobiotechnology |
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| Online Access: | https://doi.org/10.1049/iet-nbt.2019.0149 |
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| author | Sara Khan Sulaiman Faisal Dilawar Farhan Shams Maryam Zia Akhtar Nadhman |
| author_facet | Sara Khan Sulaiman Faisal Dilawar Farhan Shams Maryam Zia Akhtar Nadhman |
| author_sort | Sara Khan |
| collection | DOAJ |
| description | Hospital wastewater is a major contributor of disease‐causing microbes and the emergence of antibiotic resistant bacteria. In this study, thiolated iron‐doped nanoceria was synthesised and tested for killing of microbes from hospital effluent. These particles were designed to inhibit the efflux pumps of the bacteria found in hospital effluent with further ability to activate in visible light via iron doping thus generating tunable amount of reactive oxygen species (ROS). The quantum yield of the ROS generated by the nanoceria was 0.67 while the ROS types produced were singlet oxygen (36%), hydroxyl radical (31%) and hydroxyl ions (32%), respectively. The particles were initially synthesised through green route using Foeniculum vulgare seeds extract and were annealed at 200°C and further coated with thiolated chitosan to enhance the solubility and efflux pump inhibition. X‐ray diffraction confirmed the polycrystalline nature of nanoparticles and uniform spherical shape with 30 nm size, confirmed by scanning electron microscope. The nanoparticles exhibited 100% bactericidal activity at 100 µg/mL against all the isolated bacteria. The enhanced bactericidal effect of iron‐doped nanoceria could be attributed to efflux inhibition via thiolated chitosan as well as the production of ROS upon illumination in visible light, causing oxidative stress against microbes found in hospital effluent. |
| format | Article |
| id | doaj-art-894f7d79cc91437a9f0414938f68c45b |
| institution | Kabale University |
| issn | 1751-8741 1751-875X |
| language | English |
| publishDate | 2019-10-01 |
| publisher | Wiley |
| record_format | Article |
| series | IET Nanobiotechnology |
| spelling | doaj-art-894f7d79cc91437a9f0414938f68c45b2025-02-03T01:29:41ZengWileyIET Nanobiotechnology1751-87411751-875X2019-10-0113887587910.1049/iet-nbt.2019.0149Photo‐inactivation of bacteria in hospital effluent via thiolated iron‐doped nanoceriaSara Khan0Sulaiman Faisal1Dilawar Farhan Shams2Maryam Zia3Akhtar Nadhman4Institute of Integrative Biosciences CECOS UniversityPeshawarPakistanInstitute of Integrative Biosciences CECOS UniversityPeshawarPakistanDepartment of Environmental SciencesAbdul Wali Khan UniversityMardanPakistanInstitute of Integrative Biosciences CECOS UniversityPeshawarPakistanInstitute of Integrative Biosciences CECOS UniversityPeshawarPakistanHospital wastewater is a major contributor of disease‐causing microbes and the emergence of antibiotic resistant bacteria. In this study, thiolated iron‐doped nanoceria was synthesised and tested for killing of microbes from hospital effluent. These particles were designed to inhibit the efflux pumps of the bacteria found in hospital effluent with further ability to activate in visible light via iron doping thus generating tunable amount of reactive oxygen species (ROS). The quantum yield of the ROS generated by the nanoceria was 0.67 while the ROS types produced were singlet oxygen (36%), hydroxyl radical (31%) and hydroxyl ions (32%), respectively. The particles were initially synthesised through green route using Foeniculum vulgare seeds extract and were annealed at 200°C and further coated with thiolated chitosan to enhance the solubility and efflux pump inhibition. X‐ray diffraction confirmed the polycrystalline nature of nanoparticles and uniform spherical shape with 30 nm size, confirmed by scanning electron microscope. The nanoparticles exhibited 100% bactericidal activity at 100 µg/mL against all the isolated bacteria. The enhanced bactericidal effect of iron‐doped nanoceria could be attributed to efflux inhibition via thiolated chitosan as well as the production of ROS upon illumination in visible light, causing oxidative stress against microbes found in hospital effluent.https://doi.org/10.1049/iet-nbt.2019.0149antibiotic resistant bacteriathiolated iron‐doped nanoceriahospital effluentvisible lightreactive oxygen specieshydroxyl ions |
| spellingShingle | Sara Khan Sulaiman Faisal Dilawar Farhan Shams Maryam Zia Akhtar Nadhman Photo‐inactivation of bacteria in hospital effluent via thiolated iron‐doped nanoceria IET Nanobiotechnology antibiotic resistant bacteria thiolated iron‐doped nanoceria hospital effluent visible light reactive oxygen species hydroxyl ions |
| title | Photo‐inactivation of bacteria in hospital effluent via thiolated iron‐doped nanoceria |
| title_full | Photo‐inactivation of bacteria in hospital effluent via thiolated iron‐doped nanoceria |
| title_fullStr | Photo‐inactivation of bacteria in hospital effluent via thiolated iron‐doped nanoceria |
| title_full_unstemmed | Photo‐inactivation of bacteria in hospital effluent via thiolated iron‐doped nanoceria |
| title_short | Photo‐inactivation of bacteria in hospital effluent via thiolated iron‐doped nanoceria |
| title_sort | photo inactivation of bacteria in hospital effluent via thiolated iron doped nanoceria |
| topic | antibiotic resistant bacteria thiolated iron‐doped nanoceria hospital effluent visible light reactive oxygen species hydroxyl ions |
| url | https://doi.org/10.1049/iet-nbt.2019.0149 |
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