Fas-siRNA 对小鼠肝缺血再灌注损伤的保护作用
【目的】建立小鼠肝缺血再灌注损伤模型,探讨针对Fas基因的siRNA对小鼠肝缺血再损伤的保护作用。【方法】健康、雄性(8～10周,23～28g)昆明小鼠(N=80)分为4组,每组20只,每组按时点(30min,1、2、6和24h5个时点)随机分成5个小组(n=4)。空白组(Shamgroup)尾静脉注射PBS液1mL,8h和24h后各重复1次。麻醉和剖腹后关腹,不阻断肝门。对照组(Controlgroup)同样注射3次PBS液1mL,部分阻断肝门(约70%)30min后再灌注。GFP-siRNA组(GFPgroup)和Fas-siRNA组(Fasgroup)分别尾静脉注射3次GFP-siRNA...
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| Format: | Article |
|---|---|
| Language: | zho |
| Published: |
Editorial Office of Journal of Sun Yat-sen University
2004-01-01
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| Series: | Zhongshan Daxue xuebao. Yixue kexue ban |
| Online Access: | http://xuebaoyx.sysu.edu.cn/zh/article/43585645/ |
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