Chemical- and photo-activation of protein-protein thiol-ene coupling for protein profiling

Abstract The thiol-ene reaction between an alkene and a thiol can be exploited for selective labelling of cysteine residues in protein profiling applications. Here, we explore thiol-ene activation in systems from chemical models to complex cellular milieus, using UV, visible wavelength and redox ini...

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Bibliographic Details
Main Authors: André Campaniҫo, Marcin Baran, Andrew G. Bowie, Daniel B. Longley, Timothy Harrison, Joanna F. McGouran
Format: Article
Language:English
Published: Nature Portfolio 2025-01-01
Series:Communications Chemistry
Online Access:https://doi.org/10.1038/s42004-025-01412-6
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