Cancer Stem Cell Marker Endoglin (CD105) Induces Epithelial Mesenchymal Transition (EMT) but Not Metastasis in Clear Cell Renal Cell Carcinoma
Clear cell renal cell carcinoma (ccRCC) is the most common histological subtype of kidney cancer. We previously reported that CD105(+) subpopulation in human ccRCC tumors possesses tumor cell self-renewal and chemoresistance capability. In this study, we showed that CD105(+) ACHN tumor cells exhibit...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2019-01-01
|
| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2019/9060152 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832554505782165504 |
|---|---|
| author | Junhui Hu Wei Guan Libin Yan Zhangqun Ye Lily Wu Hua Xu |
| author_facet | Junhui Hu Wei Guan Libin Yan Zhangqun Ye Lily Wu Hua Xu |
| author_sort | Junhui Hu |
| collection | DOAJ |
| description | Clear cell renal cell carcinoma (ccRCC) is the most common histological subtype of kidney cancer. We previously reported that CD105(+) subpopulation in human ccRCC tumors possesses tumor cell self-renewal and chemoresistance capability. In this study, we showed that CD105(+) ACHN tumor cells exhibit epithelial mesenchymal transition (EMT) phenotype with high expression of mesenchymal marker N-cadherin and low expression of epithelial marker E-cadherin. They are more motile and invasive compared to the unselected parental ACHN tumor cells. The knockdown of CD105 by RNA interference led to the downregulation of N-cadherin and the upregulation of E-cadherin and reduced motility and invasiveness of CD105(+) cells. Overexpression of stem cell factor MYC in CD105 knocked down cells increased mesenchymal markers and cell motility. However, the CD105(+) population of tumor cells does not exhibit an increase metastatic potential in vivo. Findings from this study support that CD105 plays a functional role in maintaining cancer stem cell and EMT phenotype, with MYC as a common mediator for both of these traits. Our work suggests that the ability to metastasize does not coincide with the cancer stem cell or EMT function of CD105. |
| format | Article |
| id | doaj-art-87ee5d6a194b4bbbb9b6b00993bed9ac |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-87ee5d6a194b4bbbb9b6b00993bed9ac2025-02-03T05:51:25ZengWileyStem Cells International1687-966X1687-96782019-01-01201910.1155/2019/90601529060152Cancer Stem Cell Marker Endoglin (CD105) Induces Epithelial Mesenchymal Transition (EMT) but Not Metastasis in Clear Cell Renal Cell CarcinomaJunhui Hu0Wei Guan1Libin Yan2Zhangqun Ye3Lily Wu4Hua Xu5Department of Urology and Institute of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology (HUST), Wuhan 430030, ChinaDepartment of Urology and Institute of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology (HUST), Wuhan 430030, ChinaDepartment of Urology and Institute of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology (HUST), Wuhan 430030, ChinaDepartment of Urology and Institute of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology (HUST), Wuhan 430030, ChinaDepartment of Molecular and Medical Pharmacology, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles CA 90095, USADepartment of Urology and Institute of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology (HUST), Wuhan 430030, ChinaClear cell renal cell carcinoma (ccRCC) is the most common histological subtype of kidney cancer. We previously reported that CD105(+) subpopulation in human ccRCC tumors possesses tumor cell self-renewal and chemoresistance capability. In this study, we showed that CD105(+) ACHN tumor cells exhibit epithelial mesenchymal transition (EMT) phenotype with high expression of mesenchymal marker N-cadherin and low expression of epithelial marker E-cadherin. They are more motile and invasive compared to the unselected parental ACHN tumor cells. The knockdown of CD105 by RNA interference led to the downregulation of N-cadherin and the upregulation of E-cadherin and reduced motility and invasiveness of CD105(+) cells. Overexpression of stem cell factor MYC in CD105 knocked down cells increased mesenchymal markers and cell motility. However, the CD105(+) population of tumor cells does not exhibit an increase metastatic potential in vivo. Findings from this study support that CD105 plays a functional role in maintaining cancer stem cell and EMT phenotype, with MYC as a common mediator for both of these traits. Our work suggests that the ability to metastasize does not coincide with the cancer stem cell or EMT function of CD105.http://dx.doi.org/10.1155/2019/9060152 |
| spellingShingle | Junhui Hu Wei Guan Libin Yan Zhangqun Ye Lily Wu Hua Xu Cancer Stem Cell Marker Endoglin (CD105) Induces Epithelial Mesenchymal Transition (EMT) but Not Metastasis in Clear Cell Renal Cell Carcinoma Stem Cells International |
| title | Cancer Stem Cell Marker Endoglin (CD105) Induces Epithelial Mesenchymal Transition (EMT) but Not Metastasis in Clear Cell Renal Cell Carcinoma |
| title_full | Cancer Stem Cell Marker Endoglin (CD105) Induces Epithelial Mesenchymal Transition (EMT) but Not Metastasis in Clear Cell Renal Cell Carcinoma |
| title_fullStr | Cancer Stem Cell Marker Endoglin (CD105) Induces Epithelial Mesenchymal Transition (EMT) but Not Metastasis in Clear Cell Renal Cell Carcinoma |
| title_full_unstemmed | Cancer Stem Cell Marker Endoglin (CD105) Induces Epithelial Mesenchymal Transition (EMT) but Not Metastasis in Clear Cell Renal Cell Carcinoma |
| title_short | Cancer Stem Cell Marker Endoglin (CD105) Induces Epithelial Mesenchymal Transition (EMT) but Not Metastasis in Clear Cell Renal Cell Carcinoma |
| title_sort | cancer stem cell marker endoglin cd105 induces epithelial mesenchymal transition emt but not metastasis in clear cell renal cell carcinoma |
| url | http://dx.doi.org/10.1155/2019/9060152 |
| work_keys_str_mv | AT junhuihu cancerstemcellmarkerendoglincd105inducesepithelialmesenchymaltransitionemtbutnotmetastasisinclearcellrenalcellcarcinoma AT weiguan cancerstemcellmarkerendoglincd105inducesepithelialmesenchymaltransitionemtbutnotmetastasisinclearcellrenalcellcarcinoma AT libinyan cancerstemcellmarkerendoglincd105inducesepithelialmesenchymaltransitionemtbutnotmetastasisinclearcellrenalcellcarcinoma AT zhangqunye cancerstemcellmarkerendoglincd105inducesepithelialmesenchymaltransitionemtbutnotmetastasisinclearcellrenalcellcarcinoma AT lilywu cancerstemcellmarkerendoglincd105inducesepithelialmesenchymaltransitionemtbutnotmetastasisinclearcellrenalcellcarcinoma AT huaxu cancerstemcellmarkerendoglincd105inducesepithelialmesenchymaltransitionemtbutnotmetastasisinclearcellrenalcellcarcinoma |