Magnetic Nanoparticles of Chitosan for Targeted Delivery System of Plasmids to the Lungs
One of the major problems of gene therapy is the efficient, specific, and targeted delivery as well as the safety of the materials used in such systems. The specific targeted delivery of genes to the lung offers the possibility to treat a variety of specific diseases. We developed chitosan nanoparti...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | Journal of Nanotechnology |
| Online Access: | http://dx.doi.org/10.1155/2014/313415 |
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| _version_ | 1832563789393821696 |
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| author | Cynthia Aracely Alvizo Báez Itza Eloisa Luna Cruz Maria Cristina Rodríguez Padilla Juan Manuel Alcocer González |
| author_facet | Cynthia Aracely Alvizo Báez Itza Eloisa Luna Cruz Maria Cristina Rodríguez Padilla Juan Manuel Alcocer González |
| author_sort | Cynthia Aracely Alvizo Báez |
| collection | DOAJ |
| description | One of the major problems of gene therapy is the efficient, specific, and targeted delivery as well as the safety of the materials used in such systems. The specific targeted delivery of genes to the lung offers the possibility to treat a variety of specific diseases. We developed chitosan nanoparticles with the plasmid pCEM-Luc, which contains a promoter activated by magnetic field. Nanoparticles of 200–250 nm obtained by ionic gelation with a 99% retention rate were transfected in B16F10 cells and in vivo in the lungs of Balb/c mice by intratracheal administration. We observed that an external magnetic field increased the expression of the luciferase reporter gene in B16F10 cells transfected with magnetic nanoparticles and in homogenized lungs of mice which determined differences in levels of expression between different regions of the lungs (apical or distal and left or right). The highest levels of luciferase activity were observed in the apical left region. The magnetic nanoparticles prove an efficient delivery system to in vitro transfection of cells and lung tissue. |
| format | Article |
| id | doaj-art-87d951b2cf674c9f99b14ddd4b2b2555 |
| institution | Kabale University |
| issn | 1687-9503 1687-9511 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Nanotechnology |
| spelling | doaj-art-87d951b2cf674c9f99b14ddd4b2b25552025-02-03T01:12:33ZengWileyJournal of Nanotechnology1687-95031687-95112014-01-01201410.1155/2014/313415313415Magnetic Nanoparticles of Chitosan for Targeted Delivery System of Plasmids to the LungsCynthia Aracely Alvizo Báez0Itza Eloisa Luna Cruz1Maria Cristina Rodríguez Padilla2Juan Manuel Alcocer González3Laboratorio de Inmunología y Virología, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, 66450 San Nicolás de los Garza, MexicoLaboratorio de Inmunología y Virología, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, 66450 San Nicolás de los Garza, MexicoLaboratorio de Inmunología y Virología, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, 66450 San Nicolás de los Garza, MexicoLaboratorio de Inmunología y Virología, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, 66450 San Nicolás de los Garza, MexicoOne of the major problems of gene therapy is the efficient, specific, and targeted delivery as well as the safety of the materials used in such systems. The specific targeted delivery of genes to the lung offers the possibility to treat a variety of specific diseases. We developed chitosan nanoparticles with the plasmid pCEM-Luc, which contains a promoter activated by magnetic field. Nanoparticles of 200–250 nm obtained by ionic gelation with a 99% retention rate were transfected in B16F10 cells and in vivo in the lungs of Balb/c mice by intratracheal administration. We observed that an external magnetic field increased the expression of the luciferase reporter gene in B16F10 cells transfected with magnetic nanoparticles and in homogenized lungs of mice which determined differences in levels of expression between different regions of the lungs (apical or distal and left or right). The highest levels of luciferase activity were observed in the apical left region. The magnetic nanoparticles prove an efficient delivery system to in vitro transfection of cells and lung tissue.http://dx.doi.org/10.1155/2014/313415 |
| spellingShingle | Cynthia Aracely Alvizo Báez Itza Eloisa Luna Cruz Maria Cristina Rodríguez Padilla Juan Manuel Alcocer González Magnetic Nanoparticles of Chitosan for Targeted Delivery System of Plasmids to the Lungs Journal of Nanotechnology |
| title | Magnetic Nanoparticles of Chitosan for Targeted Delivery System of Plasmids to the Lungs |
| title_full | Magnetic Nanoparticles of Chitosan for Targeted Delivery System of Plasmids to the Lungs |
| title_fullStr | Magnetic Nanoparticles of Chitosan for Targeted Delivery System of Plasmids to the Lungs |
| title_full_unstemmed | Magnetic Nanoparticles of Chitosan for Targeted Delivery System of Plasmids to the Lungs |
| title_short | Magnetic Nanoparticles of Chitosan for Targeted Delivery System of Plasmids to the Lungs |
| title_sort | magnetic nanoparticles of chitosan for targeted delivery system of plasmids to the lungs |
| url | http://dx.doi.org/10.1155/2014/313415 |
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