The hypoxia adaptation strategy of the big-belly seahorses (Hippocampus abdominalis) with tuft gills
Hypoxia tolerance is a critical trait in fish breeding, however, the mechanisms underlying hypoxia regulation in the tuft gills of seahorses remain unclear. In this study, we compared the gill structures of the big-belly seahorses (Hippocampus abdominalis, BBS) with lamellae gill fish and evaluated...
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Elsevier
2025-03-01
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| Series: | Aquaculture Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S235251342400632X |
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| author | Long-Wu Jia Le-Le Zhang Hong-Wei Yang Chun-Yu Hou Lin Shi Yu-Ping Hou Guo-Dong Zheng Xiao-Lei Su Kai Wang |
| author_facet | Long-Wu Jia Le-Le Zhang Hong-Wei Yang Chun-Yu Hou Lin Shi Yu-Ping Hou Guo-Dong Zheng Xiao-Lei Su Kai Wang |
| author_sort | Long-Wu Jia |
| collection | DOAJ |
| description | Hypoxia tolerance is a critical trait in fish breeding, however, the mechanisms underlying hypoxia regulation in the tuft gills of seahorses remain unclear. In this study, we compared the gill structures of the big-belly seahorses (Hippocampus abdominalis, BBS) with lamellae gill fish and evaluated the effects of hypoxia on BBS. The average oxygen tension threshold for loss of equilibrium (LOEcrit) of BBS (2.68 mg·L−1) was higher than that of the common hypoxia-sensitive fish. Compared with the normoxia group, the duration of stationary, irregular swimming, curled tail, lying down behaviors, and respiratory rate significantly (P < 0.05) increased, while the duration of swinging and swimming behaviors significantly (P < 0.05) decreased in response to hypoxia. After 4- or 7-days of hypoxia treatment, the red blood cell counts, SOD, CAT, GSH, and T-AOC of BBS were significantly (P < 0.05) higher than those in the normoxia group. Notably, when BBS were exposed to hypoxia, the relative surface area of the exposed distal gill filaments significantly increased (P < 0.05). Meanwhile, no significant (P > 0.05) changes were observed in the medial and proximal regions. Ultimately, we identified 5 significantly (P < 0.05) down-regulated (itgav, itga6a, itga1, pcna, and adcy9) and 5 significantly (P < 0.05) up-regulated (gusb, src, cdc20, ccnb1, and plk1) core hub genes related to gill remodeling. This study demonstrates that BBS is sensitive to hypoxia, and its unique tuft gills respond to hypoxia through local gill remodel, providing valuable insights for the breeding of hypoxia-tolerance seahorses. |
| format | Article |
| id | doaj-art-874f81cc3bd24fb3948cb04fcebbd3ec |
| institution | Kabale University |
| issn | 2352-5134 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Aquaculture Reports |
| spelling | doaj-art-874f81cc3bd24fb3948cb04fcebbd3ec2025-02-06T05:12:05ZengElsevierAquaculture Reports2352-51342025-03-0140102544The hypoxia adaptation strategy of the big-belly seahorses (Hippocampus abdominalis) with tuft gillsLong-Wu Jia0Le-Le Zhang1Hong-Wei Yang2Chun-Yu Hou3Lin Shi4Yu-Ping Hou5Guo-Dong Zheng6Xiao-Lei Su7Kai Wang8School of Fisheries, Ludong University, Yantai 264025, China; Research and Development Center of Science, Technology and Industrialization of Seahorses, Ludong University, Yantai 264025, China; School of Life Sciences, Ludong University, Yantai 264025, ChinaSchool of Fisheries, Ludong University, Yantai 264025, China; Research and Development Center of Science, Technology and Industrialization of Seahorses, Ludong University, Yantai 264025, China; School of Marine Science, Ningbo University, Ningbo, China; School of Life Sciences, Ludong University, Yantai 264025, ChinaSchool of Fisheries, Ludong University, Yantai 264025, China; Research and Development Center of Science, Technology and Industrialization of Seahorses, Ludong University, Yantai 264025, China; School of Life Sciences, Ludong University, Yantai 264025, ChinaYantai Engineering & Technology College, Yantai 264025, China; School of Life Sciences, Ludong University, Yantai 264025, ChinaSchool of Fisheries, Ludong University, Yantai 264025, China; Research and Development Center of Science, Technology and Industrialization of Seahorses, Ludong University, Yantai 264025, China; School of Life Sciences, Ludong University, Yantai 264025, ChinaResearch and Development Center of Science, Technology and Industrialization of Seahorses, Ludong University, Yantai 264025, China; School of Life Sciences, Ludong University, Yantai 264025, ChinaSchool of Life Sciences, Ludong University, Yantai 264025, China; National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, ChinaSchool of Fisheries, Ludong University, Yantai 264025, China; Research and Development Center of Science, Technology and Industrialization of Seahorses, Ludong University, Yantai 264025, China; School of Life Sciences, Ludong University, Yantai 264025, China; Corresponding authors at: School of Fisheries, Ludong University, Yantai 264025, China.School of Fisheries, Ludong University, Yantai 264025, China; Research and Development Center of Science, Technology and Industrialization of Seahorses, Ludong University, Yantai 264025, China; School of Life Sciences, Ludong University, Yantai 264025, China; Corresponding authors at: School of Fisheries, Ludong University, Yantai 264025, China.Hypoxia tolerance is a critical trait in fish breeding, however, the mechanisms underlying hypoxia regulation in the tuft gills of seahorses remain unclear. In this study, we compared the gill structures of the big-belly seahorses (Hippocampus abdominalis, BBS) with lamellae gill fish and evaluated the effects of hypoxia on BBS. The average oxygen tension threshold for loss of equilibrium (LOEcrit) of BBS (2.68 mg·L−1) was higher than that of the common hypoxia-sensitive fish. Compared with the normoxia group, the duration of stationary, irregular swimming, curled tail, lying down behaviors, and respiratory rate significantly (P < 0.05) increased, while the duration of swinging and swimming behaviors significantly (P < 0.05) decreased in response to hypoxia. After 4- or 7-days of hypoxia treatment, the red blood cell counts, SOD, CAT, GSH, and T-AOC of BBS were significantly (P < 0.05) higher than those in the normoxia group. Notably, when BBS were exposed to hypoxia, the relative surface area of the exposed distal gill filaments significantly increased (P < 0.05). Meanwhile, no significant (P > 0.05) changes were observed in the medial and proximal regions. Ultimately, we identified 5 significantly (P < 0.05) down-regulated (itgav, itga6a, itga1, pcna, and adcy9) and 5 significantly (P < 0.05) up-regulated (gusb, src, cdc20, ccnb1, and plk1) core hub genes related to gill remodeling. This study demonstrates that BBS is sensitive to hypoxia, and its unique tuft gills respond to hypoxia through local gill remodel, providing valuable insights for the breeding of hypoxia-tolerance seahorses.http://www.sciencedirect.com/science/article/pii/S235251342400632XBig-belly seahorsesHypoxiaTuft gillLocal gill remodelOxidative stress |
| spellingShingle | Long-Wu Jia Le-Le Zhang Hong-Wei Yang Chun-Yu Hou Lin Shi Yu-Ping Hou Guo-Dong Zheng Xiao-Lei Su Kai Wang The hypoxia adaptation strategy of the big-belly seahorses (Hippocampus abdominalis) with tuft gills Aquaculture Reports Big-belly seahorses Hypoxia Tuft gill Local gill remodel Oxidative stress |
| title | The hypoxia adaptation strategy of the big-belly seahorses (Hippocampus abdominalis) with tuft gills |
| title_full | The hypoxia adaptation strategy of the big-belly seahorses (Hippocampus abdominalis) with tuft gills |
| title_fullStr | The hypoxia adaptation strategy of the big-belly seahorses (Hippocampus abdominalis) with tuft gills |
| title_full_unstemmed | The hypoxia adaptation strategy of the big-belly seahorses (Hippocampus abdominalis) with tuft gills |
| title_short | The hypoxia adaptation strategy of the big-belly seahorses (Hippocampus abdominalis) with tuft gills |
| title_sort | hypoxia adaptation strategy of the big belly seahorses hippocampus abdominalis with tuft gills |
| topic | Big-belly seahorses Hypoxia Tuft gill Local gill remodel Oxidative stress |
| url | http://www.sciencedirect.com/science/article/pii/S235251342400632X |
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