Biofilm Formation and blaOXA Genes Detection Among Acinetobacter baumannii from Clinical Isolates in a Tertiary Care Kirtipur Hospital, Nepal
(1) Background: Acinetobacter baumannii has emerged as a leading cause of nosocomial infections as they are capable of evolving resistance to various classes of antibiotics. The ability of A. baumannii to form biofilm might also be associated with increased antibiotic resistance and hence treatment...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
HH Publisher
2021-11-01
|
| Series: | Progress in Microbes and Molecular Biology |
| Online Access: | https://journals.hh-publisher.com/index.php/pmmb/article/view/510 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832541417918955520 |
|---|---|
| author | Upasana Ghimire Rupesh Kandel Mary Neupane Sanjit Shrestha Sudeep K C Santosh Khanal Dev Raj Joshi |
| author_facet | Upasana Ghimire Rupesh Kandel Mary Neupane Sanjit Shrestha Sudeep K C Santosh Khanal Dev Raj Joshi |
| author_sort | Upasana Ghimire |
| collection | DOAJ |
| description |
(1) Background: Acinetobacter baumannii has emerged as a leading cause of nosocomial infections as they are capable of evolving resistance to various classes of antibiotics. The ability of A. baumannii to form biofilm might also be associated with increased antibiotic resistance and hence treatment failure. This study was carried to associate the biofilm formation with the drug resistance pattern of A. baumannii and to detect blaOXA-23, blaOXA-24, and blaOXA-51 from carbapenem resistance isolates. (2) Methods: Among different clinical samples, a total of 19 Acinetobacter spp. were identified with conventional microbiological procedures. The biofilm production was determined by a quantitative adherence assay. The antimicrobial susceptibility test was carried out by the Kirby-Bauer disc diffusion method, carbapenemase production detection was confirmed by Modified Hodge Test, and target resistant genes were detected by polymerase chain reaction. (3) Results: Out of 90 clinical specimens, 64.44% (58/90) showed bacterial growth, whereas 32.75% (19/58) isolates were identified as A. baumannii. Among all A. baumannii isolates, 84.21% (16/19) were multidrug-resistance and 63.16% (12/19) carbapenem resistance phenotypically. blaOXA-51 was detected in all the isolates and blaOXA-23 was detected only in 63.16% (12/19) isolates. However, blaOXA-24 was not detected in any of the isolates. Among A. baumannii, 89.47% (17/19) isolates produced biofilm with 47.37% (9/19) strong biofilm producers. (4) Conclusions: In the majority of MDR A. baumannii, blaOXA-51 and blaOXA-23 were detected as the determinant factor for carbapenem resistance having a direct relation with biofilm formation. This study provided a valuable clue for the management of A. baumannii infections in clinical settings.
|
| format | Article |
| id | doaj-art-86a3c63358cb4a81b9316df60f2b6416 |
| institution | Kabale University |
| issn | 2637-1049 |
| language | English |
| publishDate | 2021-11-01 |
| publisher | HH Publisher |
| record_format | Article |
| series | Progress in Microbes and Molecular Biology |
| spelling | doaj-art-86a3c63358cb4a81b9316df60f2b64162025-02-04T08:40:12ZengHH PublisherProgress in Microbes and Molecular Biology2637-10492021-11-014110.36877/pmmb.a0000245Biofilm Formation and blaOXA Genes Detection Among Acinetobacter baumannii from Clinical Isolates in a Tertiary Care Kirtipur Hospital, NepalUpasana GhimireRupesh KandelMary NeupaneSanjit ShresthaSudeep K CSantosh KhanalDev Raj Joshi (1) Background: Acinetobacter baumannii has emerged as a leading cause of nosocomial infections as they are capable of evolving resistance to various classes of antibiotics. The ability of A. baumannii to form biofilm might also be associated with increased antibiotic resistance and hence treatment failure. This study was carried to associate the biofilm formation with the drug resistance pattern of A. baumannii and to detect blaOXA-23, blaOXA-24, and blaOXA-51 from carbapenem resistance isolates. (2) Methods: Among different clinical samples, a total of 19 Acinetobacter spp. were identified with conventional microbiological procedures. The biofilm production was determined by a quantitative adherence assay. The antimicrobial susceptibility test was carried out by the Kirby-Bauer disc diffusion method, carbapenemase production detection was confirmed by Modified Hodge Test, and target resistant genes were detected by polymerase chain reaction. (3) Results: Out of 90 clinical specimens, 64.44% (58/90) showed bacterial growth, whereas 32.75% (19/58) isolates were identified as A. baumannii. Among all A. baumannii isolates, 84.21% (16/19) were multidrug-resistance and 63.16% (12/19) carbapenem resistance phenotypically. blaOXA-51 was detected in all the isolates and blaOXA-23 was detected only in 63.16% (12/19) isolates. However, blaOXA-24 was not detected in any of the isolates. Among A. baumannii, 89.47% (17/19) isolates produced biofilm with 47.37% (9/19) strong biofilm producers. (4) Conclusions: In the majority of MDR A. baumannii, blaOXA-51 and blaOXA-23 were detected as the determinant factor for carbapenem resistance having a direct relation with biofilm formation. This study provided a valuable clue for the management of A. baumannii infections in clinical settings. https://journals.hh-publisher.com/index.php/pmmb/article/view/510 |
| spellingShingle | Upasana Ghimire Rupesh Kandel Mary Neupane Sanjit Shrestha Sudeep K C Santosh Khanal Dev Raj Joshi Biofilm Formation and blaOXA Genes Detection Among Acinetobacter baumannii from Clinical Isolates in a Tertiary Care Kirtipur Hospital, Nepal Progress in Microbes and Molecular Biology |
| title | Biofilm Formation and blaOXA Genes Detection Among Acinetobacter baumannii from Clinical Isolates in a Tertiary Care Kirtipur Hospital, Nepal |
| title_full | Biofilm Formation and blaOXA Genes Detection Among Acinetobacter baumannii from Clinical Isolates in a Tertiary Care Kirtipur Hospital, Nepal |
| title_fullStr | Biofilm Formation and blaOXA Genes Detection Among Acinetobacter baumannii from Clinical Isolates in a Tertiary Care Kirtipur Hospital, Nepal |
| title_full_unstemmed | Biofilm Formation and blaOXA Genes Detection Among Acinetobacter baumannii from Clinical Isolates in a Tertiary Care Kirtipur Hospital, Nepal |
| title_short | Biofilm Formation and blaOXA Genes Detection Among Acinetobacter baumannii from Clinical Isolates in a Tertiary Care Kirtipur Hospital, Nepal |
| title_sort | biofilm formation and blaoxa genes detection among acinetobacter baumannii from clinical isolates in a tertiary care kirtipur hospital nepal |
| url | https://journals.hh-publisher.com/index.php/pmmb/article/view/510 |
| work_keys_str_mv | AT upasanaghimire biofilmformationandblaoxagenesdetectionamongacinetobacterbaumanniifromclinicalisolatesinatertiarycarekirtipurhospitalnepal AT rupeshkandel biofilmformationandblaoxagenesdetectionamongacinetobacterbaumanniifromclinicalisolatesinatertiarycarekirtipurhospitalnepal AT maryneupane biofilmformationandblaoxagenesdetectionamongacinetobacterbaumanniifromclinicalisolatesinatertiarycarekirtipurhospitalnepal AT sanjitshrestha biofilmformationandblaoxagenesdetectionamongacinetobacterbaumanniifromclinicalisolatesinatertiarycarekirtipurhospitalnepal AT sudeepkc biofilmformationandblaoxagenesdetectionamongacinetobacterbaumanniifromclinicalisolatesinatertiarycarekirtipurhospitalnepal AT santoshkhanal biofilmformationandblaoxagenesdetectionamongacinetobacterbaumanniifromclinicalisolatesinatertiarycarekirtipurhospitalnepal AT devrajjoshi biofilmformationandblaoxagenesdetectionamongacinetobacterbaumanniifromclinicalisolatesinatertiarycarekirtipurhospitalnepal |