Characterization of Cellulolytic Bacterial Cultures Grown in Different Substrates
Nine aerobic cellulolytic bacterial cultures were obtained from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Culture (DSMZ) and the American Type Culture Collection (ATCC). The objectives of this study were to characterize the cellulolytic bacteria and to determine the opt...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2013/689235 |
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| author | Mohamed Idris Alshelmani Teck Chwen Loh Hooi Ling Foo Wei Hong Lau Awis Qurni Sazili |
| author_facet | Mohamed Idris Alshelmani Teck Chwen Loh Hooi Ling Foo Wei Hong Lau Awis Qurni Sazili |
| author_sort | Mohamed Idris Alshelmani |
| collection | DOAJ |
| description | Nine aerobic cellulolytic bacterial cultures were obtained from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Culture (DSMZ) and the American Type Culture Collection (ATCC). The objectives of this study were to characterize the cellulolytic bacteria and to determine the optimum moisture ratio required for solid state fermentation (SSF) of palm kernel cake (PKC). The bacteria cultures were grown on reconstituted nutrient broth, incubated at 30∘C and agitated at 200 rpm. Carboxymethyl cellulase, xylanase, and mannanase activities were determined using different substrates and after SSF of PKC. The SSF was conducted for 4 and 7 days with inoculum size of 10% (v/w) on different PKC concentration-to-moisture ratios: 1 : 0.2, 1 : 0.3, 1 : 0.4, and 1 : 0.5. Results showed that Bacillus amyloliquefaciens 1067 DSMZ, Bacillus megaterium 9885 ATCC, Paenibacillus curdlanolyticus 10248 DSMZ, and Paenibacillus polymyxa 842 ATCC produced higher enzyme activities as compared to other bacterial cultures grown on different substrates. The cultures mentioned above also produced higher enzyme activities when they were incubated under SSF using PKC as a substrate in different PKC-to-moisture ratios after 4 days of incubation, indicating that these cellulolytic bacteria can be used to degrade and improve the nutrient quality of PKC. |
| format | Article |
| id | doaj-art-863bd9c9c8b043b58a5c9e091904f7bb |
| institution | Kabale University |
| issn | 1537-744X |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
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| series | The Scientific World Journal |
| spelling | doaj-art-863bd9c9c8b043b58a5c9e091904f7bb2025-02-03T01:28:46ZengWileyThe Scientific World Journal1537-744X2013-01-01201310.1155/2013/689235689235Characterization of Cellulolytic Bacterial Cultures Grown in Different SubstratesMohamed Idris Alshelmani0Teck Chwen Loh1Hooi Ling Foo2Wei Hong Lau3Awis Qurni Sazili4Department of Animal Science, Faculty of Agriculture, Universiti Putra Malaysia (UPM), 43400 Serdang, MalaysiaDepartment of Animal Science, Faculty of Agriculture, Universiti Putra Malaysia (UPM), 43400 Serdang, MalaysiaDepartment of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, UPM, 43400 Serdang, MalaysiaDepartment of Plant Protection, Faculty of Agriculture, Universiti Putra Malaysia (UPM), 43400 Serdang, MalaysiaDepartment of Animal Science, Faculty of Agriculture, Universiti Putra Malaysia (UPM), 43400 Serdang, MalaysiaNine aerobic cellulolytic bacterial cultures were obtained from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Culture (DSMZ) and the American Type Culture Collection (ATCC). The objectives of this study were to characterize the cellulolytic bacteria and to determine the optimum moisture ratio required for solid state fermentation (SSF) of palm kernel cake (PKC). The bacteria cultures were grown on reconstituted nutrient broth, incubated at 30∘C and agitated at 200 rpm. Carboxymethyl cellulase, xylanase, and mannanase activities were determined using different substrates and after SSF of PKC. The SSF was conducted for 4 and 7 days with inoculum size of 10% (v/w) on different PKC concentration-to-moisture ratios: 1 : 0.2, 1 : 0.3, 1 : 0.4, and 1 : 0.5. Results showed that Bacillus amyloliquefaciens 1067 DSMZ, Bacillus megaterium 9885 ATCC, Paenibacillus curdlanolyticus 10248 DSMZ, and Paenibacillus polymyxa 842 ATCC produced higher enzyme activities as compared to other bacterial cultures grown on different substrates. The cultures mentioned above also produced higher enzyme activities when they were incubated under SSF using PKC as a substrate in different PKC-to-moisture ratios after 4 days of incubation, indicating that these cellulolytic bacteria can be used to degrade and improve the nutrient quality of PKC.http://dx.doi.org/10.1155/2013/689235 |
| spellingShingle | Mohamed Idris Alshelmani Teck Chwen Loh Hooi Ling Foo Wei Hong Lau Awis Qurni Sazili Characterization of Cellulolytic Bacterial Cultures Grown in Different Substrates The Scientific World Journal |
| title | Characterization of Cellulolytic Bacterial Cultures Grown in Different Substrates |
| title_full | Characterization of Cellulolytic Bacterial Cultures Grown in Different Substrates |
| title_fullStr | Characterization of Cellulolytic Bacterial Cultures Grown in Different Substrates |
| title_full_unstemmed | Characterization of Cellulolytic Bacterial Cultures Grown in Different Substrates |
| title_short | Characterization of Cellulolytic Bacterial Cultures Grown in Different Substrates |
| title_sort | characterization of cellulolytic bacterial cultures grown in different substrates |
| url | http://dx.doi.org/10.1155/2013/689235 |
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