Pulmonary Bacterial Communities in Surgically Resected Noncystic Fibrosis Bronchiectasis Lungs Are Similar to Those in Cystic Fibrosis
Background. Recurrent bacterial infections play a key role in the pathogenesis of bronchiectasis, but conventional microbiologic methods may fail to identify pathogens in many cases. We characterized and compared the pulmonary bacterial communities of cystic fibrosis (CF) and non-CF bronchiectasis p...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | Pulmonary Medicine |
| Online Access: | http://dx.doi.org/10.1155/2012/746358 |
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| author | Heather Maughan Kristopher S. Cunningham Pauline W. Wang Yu Zhang Marcelo Cypel Cecilia Chaparro D. Elizabeth Tullis Thomas K. Waddell Shaf Keshavjee Mingyao Liu David S. Guttman David M. Hwang |
| author_facet | Heather Maughan Kristopher S. Cunningham Pauline W. Wang Yu Zhang Marcelo Cypel Cecilia Chaparro D. Elizabeth Tullis Thomas K. Waddell Shaf Keshavjee Mingyao Liu David S. Guttman David M. Hwang |
| author_sort | Heather Maughan |
| collection | DOAJ |
| description | Background. Recurrent bacterial infections play a key role in the pathogenesis of bronchiectasis, but conventional microbiologic methods may fail to identify pathogens in many cases. We characterized and compared the pulmonary bacterial communities of cystic fibrosis (CF) and non-CF bronchiectasis patients using a culture-independent molecular approach. Methods. Bacterial 16S rRNA gene libraries were constructed from lung tissue of 10 non-CF bronchiectasis and 21 CF patients, followed by DNA sequencing of isolates from each library. Community characteristics were analyzed and compared between the two groups. Results. A wide range of bacterial diversity was detected in both groups, with between 1 and 21 bacterial taxa found in each patient. Pseudomonas was the most common genus in both groups, comprising 49% of sequences detected and dominating numerically in 13 patients. Although Pseudomonas appeared to be dominant more often in CF patients than in non-CF patients, analysis of entire bacterial communities did not identify significant differences between these two groups. Conclusions. Our data indicate significant diversity in the pulmonary bacterial community of both CF and non-CF bronchiectasis patients and suggest that this community is similar in surgically resected lungs of CF and non-CF bronchiectasis patients. |
| format | Article |
| id | doaj-art-86224a70d28e4b879061eb5d60ba2bef |
| institution | Kabale University |
| issn | 2090-1836 2090-1844 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Pulmonary Medicine |
| spelling | doaj-art-86224a70d28e4b879061eb5d60ba2bef2025-02-03T01:31:38ZengWileyPulmonary Medicine2090-18362090-18442012-01-01201210.1155/2012/746358746358Pulmonary Bacterial Communities in Surgically Resected Noncystic Fibrosis Bronchiectasis Lungs Are Similar to Those in Cystic FibrosisHeather Maughan0Kristopher S. Cunningham1Pauline W. Wang2Yu Zhang3Marcelo Cypel4Cecilia Chaparro5D. Elizabeth Tullis6Thomas K. Waddell7Shaf Keshavjee8Mingyao Liu9David S. Guttman10David M. Hwang11Centre for the Analysis of Genome Evolution & Function, University of Toronto, Toronto, ON, M5S 3B2, CanadaDepartment of Laboratory Medicine & Pathobiology, University of Toronto, Toronto, ON, M5S 1A8, CanadaCentre for the Analysis of Genome Evolution & Function, University of Toronto, Toronto, ON, M5S 3B2, CanadaLatner Thoracic Surgery Research Laboratories, University Health Network, Toronto, ON, CanadaLatner Thoracic Surgery Research Laboratories, University Health Network, Toronto, ON, CanadaDivision of Respirology, St. Michael’s Hospital and University of Toronto, Toronto, ON, CanadaDivision of Respirology, St. Michael’s Hospital and University of Toronto, Toronto, ON, CanadaLatner Thoracic Surgery Research Laboratories, University Health Network, Toronto, ON, CanadaLatner Thoracic Surgery Research Laboratories, University Health Network, Toronto, ON, CanadaLatner Thoracic Surgery Research Laboratories, University Health Network, Toronto, ON, CanadaCentre for the Analysis of Genome Evolution & Function, University of Toronto, Toronto, ON, M5S 3B2, CanadaDepartment of Laboratory Medicine & Pathobiology, University of Toronto, Toronto, ON, M5S 1A8, CanadaBackground. Recurrent bacterial infections play a key role in the pathogenesis of bronchiectasis, but conventional microbiologic methods may fail to identify pathogens in many cases. We characterized and compared the pulmonary bacterial communities of cystic fibrosis (CF) and non-CF bronchiectasis patients using a culture-independent molecular approach. Methods. Bacterial 16S rRNA gene libraries were constructed from lung tissue of 10 non-CF bronchiectasis and 21 CF patients, followed by DNA sequencing of isolates from each library. Community characteristics were analyzed and compared between the two groups. Results. A wide range of bacterial diversity was detected in both groups, with between 1 and 21 bacterial taxa found in each patient. Pseudomonas was the most common genus in both groups, comprising 49% of sequences detected and dominating numerically in 13 patients. Although Pseudomonas appeared to be dominant more often in CF patients than in non-CF patients, analysis of entire bacterial communities did not identify significant differences between these two groups. Conclusions. Our data indicate significant diversity in the pulmonary bacterial community of both CF and non-CF bronchiectasis patients and suggest that this community is similar in surgically resected lungs of CF and non-CF bronchiectasis patients.http://dx.doi.org/10.1155/2012/746358 |
| spellingShingle | Heather Maughan Kristopher S. Cunningham Pauline W. Wang Yu Zhang Marcelo Cypel Cecilia Chaparro D. Elizabeth Tullis Thomas K. Waddell Shaf Keshavjee Mingyao Liu David S. Guttman David M. Hwang Pulmonary Bacterial Communities in Surgically Resected Noncystic Fibrosis Bronchiectasis Lungs Are Similar to Those in Cystic Fibrosis Pulmonary Medicine |
| title | Pulmonary Bacterial Communities in Surgically Resected Noncystic Fibrosis Bronchiectasis Lungs Are Similar to Those in Cystic Fibrosis |
| title_full | Pulmonary Bacterial Communities in Surgically Resected Noncystic Fibrosis Bronchiectasis Lungs Are Similar to Those in Cystic Fibrosis |
| title_fullStr | Pulmonary Bacterial Communities in Surgically Resected Noncystic Fibrosis Bronchiectasis Lungs Are Similar to Those in Cystic Fibrosis |
| title_full_unstemmed | Pulmonary Bacterial Communities in Surgically Resected Noncystic Fibrosis Bronchiectasis Lungs Are Similar to Those in Cystic Fibrosis |
| title_short | Pulmonary Bacterial Communities in Surgically Resected Noncystic Fibrosis Bronchiectasis Lungs Are Similar to Those in Cystic Fibrosis |
| title_sort | pulmonary bacterial communities in surgically resected noncystic fibrosis bronchiectasis lungs are similar to those in cystic fibrosis |
| url | http://dx.doi.org/10.1155/2012/746358 |
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