The crystal structure of GH57 family amylopullulanase reveals its dual binding pockets sharing the same catalytic dyad

Abstract Glycoside Hydrolase Family 57 (GH57) amylopullulanase is a thermophilic endoamylase capable of hydrolyzing both α-1,4 and α-1,6-glycosidic bonds, demonstrating significant potential for one-step starch saccharification in industrial applications. However, the mechanisms underlying the dual...

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Bibliographic Details
Main Authors: Zhimin Zhu, Weiwei Wang, Minjun Li, Qin Xu, Huan Zhou, Liqing Huang, Qisheng Wang, Feng Yu
Format: Article
Language:English
Published: Nature Portfolio 2025-05-01
Series:Communications Biology
Online Access:https://doi.org/10.1038/s42003-025-08192-8
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