Sustained-Release Fillers for Dentin Disinfection: An Ex Vivo Study
Enterococcus faecalis is the most commonly recovered species from failed root canal treatments. In this study, we tested the capability of a novel intracanal sustained-release filler (SRF) containing cetylpyridinium chloride (CPC) to disinfect dentinal tubules of segmented human tooth specimens. Hum...
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| Format: | Article |
| Language: | English |
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Wiley
2019-01-01
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| Series: | International Journal of Dentistry |
| Online Access: | http://dx.doi.org/10.1155/2019/2348146 |
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| author | Bernhard Funk Sharonit Sahar-Helft David Kirmayer Michael Friedman Doron Steinberg |
| author_facet | Bernhard Funk Sharonit Sahar-Helft David Kirmayer Michael Friedman Doron Steinberg |
| author_sort | Bernhard Funk |
| collection | DOAJ |
| description | Enterococcus faecalis is the most commonly recovered species from failed root canal treatments. In this study, we tested the capability of a novel intracanal sustained-release filler (SRF) containing cetylpyridinium chloride (CPC) to disinfect dentinal tubules of segmented human tooth specimens. Human dental root specimens were infected with E. faecalis V583 for 3 weeks in a static environment. The tested intracanal medicaments were SRF-CPC and calcium hydroxide (CH). Each medicament was introduced into the canal of the dental specimen and incubated for 7 days. The bacteriological samples were taken by shaving the dentine surrounding the root canal with dental burs ranging in size from ISO 014-020. The obtained dentine powder was collected in test tubes containing phosphate-buffered saline, sonicated, and plated on agar plates. Colony-forming units were counted after 48 h of incubation. Random specimens were also examined under confocal laser scanning microscopy and scanning electron microscopy. A statistical difference was found in the bacterial counts obtained from all layers of infected dentin between the control and the SRF-CPC groups. CH reduced bacterial viability significantly only in the first layer of the infected dentin, up to 150 μm into the dentinal tubules. CLSM images showed that SRF-CPC killed most bacteria throughout the infected dentin up to 700 μm of penetration. SEM images demonstrated the adhesion ability of SRF-CPC to the dentinal wall. In conclusion, SRF-CPC is a potential intracanal medicament for disinfecting dentinal tubules. |
| format | Article |
| id | doaj-art-8590e7fe1a9243bebf871d735db3d72a |
| institution | Kabale University |
| issn | 1687-8728 1687-8736 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Dentistry |
| spelling | doaj-art-8590e7fe1a9243bebf871d735db3d72a2025-02-03T05:51:51ZengWileyInternational Journal of Dentistry1687-87281687-87362019-01-01201910.1155/2019/23481462348146Sustained-Release Fillers for Dentin Disinfection: An Ex Vivo StudyBernhard Funk0Sharonit Sahar-Helft1David Kirmayer2Michael Friedman3Doron Steinberg4The Institute of Dental Sciences, Faculty of Dental Medicine, The Hebrew University of Jerusalem, Jerusalem, IsraelDepartment of Endodontics, Faculty of Dental Medicine, The Hebrew University of Jerusalem, Jerusalem, IsraelThe Institute of Drug Research, School of Pharmacy, Faculty of Medicine, The Hebrew University of Jerusalem, Jerusalem, IsraelThe Institute of Drug Research, School of Pharmacy, Faculty of Medicine, The Hebrew University of Jerusalem, Jerusalem, IsraelThe Institute of Dental Sciences, Faculty of Dental Medicine, The Hebrew University of Jerusalem, Jerusalem, IsraelEnterococcus faecalis is the most commonly recovered species from failed root canal treatments. In this study, we tested the capability of a novel intracanal sustained-release filler (SRF) containing cetylpyridinium chloride (CPC) to disinfect dentinal tubules of segmented human tooth specimens. Human dental root specimens were infected with E. faecalis V583 for 3 weeks in a static environment. The tested intracanal medicaments were SRF-CPC and calcium hydroxide (CH). Each medicament was introduced into the canal of the dental specimen and incubated for 7 days. The bacteriological samples were taken by shaving the dentine surrounding the root canal with dental burs ranging in size from ISO 014-020. The obtained dentine powder was collected in test tubes containing phosphate-buffered saline, sonicated, and plated on agar plates. Colony-forming units were counted after 48 h of incubation. Random specimens were also examined under confocal laser scanning microscopy and scanning electron microscopy. A statistical difference was found in the bacterial counts obtained from all layers of infected dentin between the control and the SRF-CPC groups. CH reduced bacterial viability significantly only in the first layer of the infected dentin, up to 150 μm into the dentinal tubules. CLSM images showed that SRF-CPC killed most bacteria throughout the infected dentin up to 700 μm of penetration. SEM images demonstrated the adhesion ability of SRF-CPC to the dentinal wall. In conclusion, SRF-CPC is a potential intracanal medicament for disinfecting dentinal tubules.http://dx.doi.org/10.1155/2019/2348146 |
| spellingShingle | Bernhard Funk Sharonit Sahar-Helft David Kirmayer Michael Friedman Doron Steinberg Sustained-Release Fillers for Dentin Disinfection: An Ex Vivo Study International Journal of Dentistry |
| title | Sustained-Release Fillers for Dentin Disinfection: An Ex Vivo Study |
| title_full | Sustained-Release Fillers for Dentin Disinfection: An Ex Vivo Study |
| title_fullStr | Sustained-Release Fillers for Dentin Disinfection: An Ex Vivo Study |
| title_full_unstemmed | Sustained-Release Fillers for Dentin Disinfection: An Ex Vivo Study |
| title_short | Sustained-Release Fillers for Dentin Disinfection: An Ex Vivo Study |
| title_sort | sustained release fillers for dentin disinfection an ex vivo study |
| url | http://dx.doi.org/10.1155/2019/2348146 |
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