Acoustic Transmitted Decellularized Fish Bladder for Tympanic Membrane Regeneration
Developing advanced tissue-engineered membranes with biocompatibility, suitable mechanical qualities, and anti-fibrotic and anti-inflammatory actions is important for tympanic membrane (TM) repair. Here, we present a novel acoustically transmitted decellularized fish swim bladder (DFB) loaded with m...
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| Format: | Article |
| Language: | English |
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American Association for the Advancement of Science (AAAS)
2025-02-01
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| Series: | Research |
| Online Access: | https://spj.science.org/doi/10.34133/research.0596 |
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| author | Hong Chen Hui Zhang Guangjie Zhu Long Cao Chenjie Yu Maoli Duan Xiaoyun Qian Xia Gao Yuanjin Zhao |
| author_facet | Hong Chen Hui Zhang Guangjie Zhu Long Cao Chenjie Yu Maoli Duan Xiaoyun Qian Xia Gao Yuanjin Zhao |
| author_sort | Hong Chen |
| collection | DOAJ |
| description | Developing advanced tissue-engineered membranes with biocompatibility, suitable mechanical qualities, and anti-fibrotic and anti-inflammatory actions is important for tympanic membrane (TM) repair. Here, we present a novel acoustically transmitted decellularized fish swim bladder (DFB) loaded with mesenchymal stem cells (DFB@MSCs) for TM perforation (TMP) repair. The DFB scaffolds are obtained by removing the cellular components from the original FB, which retains the collagen composition that favors cell proliferation. Benefitting from their spatially porous structures and excellent mechanical properties, the DFB scaffolds can provide a suitable microenvironment and mechanical support for cell growth and tissue regeneration. In addition, by loading mesenchymal stem cells on the DFB scaffolds, the resultant DFB@MSCs system exhibits remarkable anti-fibrotic and anti-inflammatory effects, together with the ability to promote cell migration and angiogenesis. In vivo experiments confirm that the prepared DFB@MSCs scaffolds can not only alleviate inflammatory response caused by TMP but also promote new vessel formation, TM repair, and hearing improvement. These features indicate that our proposed DFB@MSCs stent is a prospective tool for the clinical repair of TM. |
| format | Article |
| id | doaj-art-84eac6269f224356b1b3e08c477d6b3c |
| institution | Kabale University |
| issn | 2639-5274 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | American Association for the Advancement of Science (AAAS) |
| record_format | Article |
| series | Research |
| spelling | doaj-art-84eac6269f224356b1b3e08c477d6b3c2025-02-05T08:00:31ZengAmerican Association for the Advancement of Science (AAAS)Research2639-52742025-02-01202510.34133/research.0596Acoustic Transmitted Decellularized Fish Bladder for Tympanic Membrane RegenerationHong Chen0Hui Zhang1Guangjie Zhu2Long Cao3Chenjie Yu4Maoli Duan5Xiaoyun Qian6Xia Gao7Yuanjin Zhao8Department of Otolaryngology Head and Neck Surgery, Nanjing Drum Tower Hospital, Research Institute of Otolaryngology, Jiangsu Provincial Key Medical Discipline, Nanjing University Medical School, Nanjing 210008, China.School of Life Sciences and Technology, Southeast University, Nanjing 210096, China.Department of Otolaryngology Head and Neck Surgery, Nanjing Drum Tower Hospital, Research Institute of Otolaryngology, Jiangsu Provincial Key Medical Discipline, Nanjing University Medical School, Nanjing 210008, China.Institute of Translational Medicine, Medical College, Yangzhou University, Yangzhou 225001, Jiangsu, China.Department of Otolaryngology Head and Neck Surgery, Nanjing Drum Tower Hospital, Research Institute of Otolaryngology, Jiangsu Provincial Key Medical Discipline, Nanjing University Medical School, Nanjing 210008, China.Department of Otolaryngology Head and Neck Surgery & Audiology and Neurotology, Karolinska University Hospital, Karolinska Institute, 171 76 Stockholm, Sweden.Department of Otolaryngology Head and Neck Surgery, Nanjing Drum Tower Hospital, Research Institute of Otolaryngology, Jiangsu Provincial Key Medical Discipline, Nanjing University Medical School, Nanjing 210008, China.Department of Otolaryngology Head and Neck Surgery, Nanjing Drum Tower Hospital, Research Institute of Otolaryngology, Jiangsu Provincial Key Medical Discipline, Nanjing University Medical School, Nanjing 210008, China.Department of Otolaryngology Head and Neck Surgery, Nanjing Drum Tower Hospital, Research Institute of Otolaryngology, Jiangsu Provincial Key Medical Discipline, Nanjing University Medical School, Nanjing 210008, China.Developing advanced tissue-engineered membranes with biocompatibility, suitable mechanical qualities, and anti-fibrotic and anti-inflammatory actions is important for tympanic membrane (TM) repair. Here, we present a novel acoustically transmitted decellularized fish swim bladder (DFB) loaded with mesenchymal stem cells (DFB@MSCs) for TM perforation (TMP) repair. The DFB scaffolds are obtained by removing the cellular components from the original FB, which retains the collagen composition that favors cell proliferation. Benefitting from their spatially porous structures and excellent mechanical properties, the DFB scaffolds can provide a suitable microenvironment and mechanical support for cell growth and tissue regeneration. In addition, by loading mesenchymal stem cells on the DFB scaffolds, the resultant DFB@MSCs system exhibits remarkable anti-fibrotic and anti-inflammatory effects, together with the ability to promote cell migration and angiogenesis. In vivo experiments confirm that the prepared DFB@MSCs scaffolds can not only alleviate inflammatory response caused by TMP but also promote new vessel formation, TM repair, and hearing improvement. These features indicate that our proposed DFB@MSCs stent is a prospective tool for the clinical repair of TM.https://spj.science.org/doi/10.34133/research.0596 |
| spellingShingle | Hong Chen Hui Zhang Guangjie Zhu Long Cao Chenjie Yu Maoli Duan Xiaoyun Qian Xia Gao Yuanjin Zhao Acoustic Transmitted Decellularized Fish Bladder for Tympanic Membrane Regeneration Research |
| title | Acoustic Transmitted Decellularized Fish Bladder for Tympanic Membrane Regeneration |
| title_full | Acoustic Transmitted Decellularized Fish Bladder for Tympanic Membrane Regeneration |
| title_fullStr | Acoustic Transmitted Decellularized Fish Bladder for Tympanic Membrane Regeneration |
| title_full_unstemmed | Acoustic Transmitted Decellularized Fish Bladder for Tympanic Membrane Regeneration |
| title_short | Acoustic Transmitted Decellularized Fish Bladder for Tympanic Membrane Regeneration |
| title_sort | acoustic transmitted decellularized fish bladder for tympanic membrane regeneration |
| url | https://spj.science.org/doi/10.34133/research.0596 |
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