Cell Counts, rather than Proportion, of CD8/PD-1 Tumor-Infiltrating Lymphocytes in a Tumor Microenvironment Associated with Pathological Characteristics of Chinese Invasive Ductal Breast Cancer
Objective. This study is aimed at investigating the association of exhausted CD8+ tumor-infiltrating lymphocytes with clinic-pathological factors. Methods. 133 patients diagnosed with primary invasive ductal breast cancer were recruited into the cross-sectional study consecutively. Immunohistochemis...
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| Format: | Article |
| Language: | English |
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Wiley
2019-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2019/8505021 |
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| author | Qingkun Song Feng Shi Maya Adair Hong Chang Xiudong Guan Yanjie Zhao Yuchen Li Guangjiang Wu Jiangping Wu |
| author_facet | Qingkun Song Feng Shi Maya Adair Hong Chang Xiudong Guan Yanjie Zhao Yuchen Li Guangjiang Wu Jiangping Wu |
| author_sort | Qingkun Song |
| collection | DOAJ |
| description | Objective. This study is aimed at investigating the association of exhausted CD8+ tumor-infiltrating lymphocytes with clinic-pathological factors. Methods. 133 patients diagnosed with primary invasive ductal breast cancer were recruited into the cross-sectional study consecutively. Immunohistochemistry was used to detect biomarker expression on formalin-fixed and paraffin-embedded sections. Double staining of CD8 and PD-1 was conducted on lymphocytes. Results. The proportion of CD8+/PD-1- TILs was 16% among patients with axillary lymph node metastasis, significantly lower than those without metastasis (24%). The expression of CK7, CK20, or Ki-67 was not related with the proportion of phenotypes of CD8/PD-1 TILs. Younger patients had more cell counts of CD8+/PD-1- TILs than elderly patients (18/HPF vs. 9/HPF, p<0.05). Patients with axillary lymph node metastasis had less CD8+/PD-1- TILs than those without metastasis (11/HPF vs. 27/HPF, p<0.05). Median counts of CD8+/PD-1- TILs among patients with CK20 and E-Cad expression were 33/HPF and 14/HPF, significantly higher than those among patients with negative CK20 (16/HPF) and E-Cad expression (6/HPF). Ki-67 index had a significant correlation with cell counts of CD8+/PD-1+ TILs and CD8+/PD-1- TILs, and the correlation coefficients were 0.19 and 0.21 (p<0.05), respectively. Conclusion. The proportion of CD8+/PD-1- TILs was related with metastatic status of the axillary lymph node but cell counts of CD8+/PD-1- TILs were related with metastatic status of the axillary lymph node and expression of CK7, CK20, E-Cad, and Ki-67. Absolute cell counts, not proportion of CD8/PD-1 TILs, were more likely to distinguish clinic and pathologic characteristics of breast cancer. |
| format | Article |
| id | doaj-art-84d6d9e8de774025aedc8cb43f0eae21 |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-84d6d9e8de774025aedc8cb43f0eae212025-02-03T06:04:59ZengWileyJournal of Immunology Research2314-88612314-71562019-01-01201910.1155/2019/85050218505021Cell Counts, rather than Proportion, of CD8/PD-1 Tumor-Infiltrating Lymphocytes in a Tumor Microenvironment Associated with Pathological Characteristics of Chinese Invasive Ductal Breast CancerQingkun Song0Feng Shi1Maya Adair2Hong Chang3Xiudong Guan4Yanjie Zhao5Yuchen Li6Guangjiang Wu7Jiangping Wu8Department of Science and Technology, Beijing Shijitan Hospital, Capital Medical University, Beijing 100038, ChinaDepartment of Pathology, Beijing Shijitan Hospital, Capital Medical University, Beijing 100038, ChinaUniversity of the Incarnate Word, San Antonio, TX 78209, USADepartment of Pathology, Beijing Shijitan Hospital, Capital Medical University, Beijing 100038, ChinaBeijing Tiantan Hospital, Capital Medical University, Beijing 100050, ChinaDepartment of Medical Oncology, Beijing Shijitan Hospital, Capital Medical University, Beijing 100038, ChinaSid Faithfull Brain Cancer Research Laboratory, QIMR Berghofer Medical Research Institute, Locked Bag 2000 Royal Brisbane Hospital, QLD 4029, AustraliaDepartment of Infection Control, Beijing Shijitan Hospital, Capital Medical University, Beijing 100038, ChinaDepartment of Cancer Research, Beijing Shijitan Hospital, Capital Medical University, Beijing 100038, ChinaObjective. This study is aimed at investigating the association of exhausted CD8+ tumor-infiltrating lymphocytes with clinic-pathological factors. Methods. 133 patients diagnosed with primary invasive ductal breast cancer were recruited into the cross-sectional study consecutively. Immunohistochemistry was used to detect biomarker expression on formalin-fixed and paraffin-embedded sections. Double staining of CD8 and PD-1 was conducted on lymphocytes. Results. The proportion of CD8+/PD-1- TILs was 16% among patients with axillary lymph node metastasis, significantly lower than those without metastasis (24%). The expression of CK7, CK20, or Ki-67 was not related with the proportion of phenotypes of CD8/PD-1 TILs. Younger patients had more cell counts of CD8+/PD-1- TILs than elderly patients (18/HPF vs. 9/HPF, p<0.05). Patients with axillary lymph node metastasis had less CD8+/PD-1- TILs than those without metastasis (11/HPF vs. 27/HPF, p<0.05). Median counts of CD8+/PD-1- TILs among patients with CK20 and E-Cad expression were 33/HPF and 14/HPF, significantly higher than those among patients with negative CK20 (16/HPF) and E-Cad expression (6/HPF). Ki-67 index had a significant correlation with cell counts of CD8+/PD-1+ TILs and CD8+/PD-1- TILs, and the correlation coefficients were 0.19 and 0.21 (p<0.05), respectively. Conclusion. The proportion of CD8+/PD-1- TILs was related with metastatic status of the axillary lymph node but cell counts of CD8+/PD-1- TILs were related with metastatic status of the axillary lymph node and expression of CK7, CK20, E-Cad, and Ki-67. Absolute cell counts, not proportion of CD8/PD-1 TILs, were more likely to distinguish clinic and pathologic characteristics of breast cancer.http://dx.doi.org/10.1155/2019/8505021 |
| spellingShingle | Qingkun Song Feng Shi Maya Adair Hong Chang Xiudong Guan Yanjie Zhao Yuchen Li Guangjiang Wu Jiangping Wu Cell Counts, rather than Proportion, of CD8/PD-1 Tumor-Infiltrating Lymphocytes in a Tumor Microenvironment Associated with Pathological Characteristics of Chinese Invasive Ductal Breast Cancer Journal of Immunology Research |
| title | Cell Counts, rather than Proportion, of CD8/PD-1 Tumor-Infiltrating Lymphocytes in a Tumor Microenvironment Associated with Pathological Characteristics of Chinese Invasive Ductal Breast Cancer |
| title_full | Cell Counts, rather than Proportion, of CD8/PD-1 Tumor-Infiltrating Lymphocytes in a Tumor Microenvironment Associated with Pathological Characteristics of Chinese Invasive Ductal Breast Cancer |
| title_fullStr | Cell Counts, rather than Proportion, of CD8/PD-1 Tumor-Infiltrating Lymphocytes in a Tumor Microenvironment Associated with Pathological Characteristics of Chinese Invasive Ductal Breast Cancer |
| title_full_unstemmed | Cell Counts, rather than Proportion, of CD8/PD-1 Tumor-Infiltrating Lymphocytes in a Tumor Microenvironment Associated with Pathological Characteristics of Chinese Invasive Ductal Breast Cancer |
| title_short | Cell Counts, rather than Proportion, of CD8/PD-1 Tumor-Infiltrating Lymphocytes in a Tumor Microenvironment Associated with Pathological Characteristics of Chinese Invasive Ductal Breast Cancer |
| title_sort | cell counts rather than proportion of cd8 pd 1 tumor infiltrating lymphocytes in a tumor microenvironment associated with pathological characteristics of chinese invasive ductal breast cancer |
| url | http://dx.doi.org/10.1155/2019/8505021 |
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