Impact of predictive selection of LbCas12a CRISPR RNAs upon on‐ and off‐target editing rates in soybean

Abstract Clustered regularly interspaced short palindromic repeats (CRISPR) technology has revolutionized creating targeted genetic variation in crops. Although CRISPR enzymes have been reported to have high sequence‐specificity, careful design of the editing reagents can also reduce unintended edit...

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Bibliographic Details
Main Authors: Linda Rymarquis, Chenxi Wu, Diane Hohorst, Miguel Vega‐Sanchez, Thomas E. Mullen, Vijetha Vemulapalli, Douglas R. Smith
Format: Article
Language:English
Published: Wiley 2024-08-01
Series:Plant Direct
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Online Access:https://doi.org/10.1002/pld3.627
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