In vitro probiotic properties of Lacticaseibacillus casei CB054 from anaerobically fermented bovine colostrums
ABSTRACT: This study evaluated Lacticaseibacillus casei CB054 (Lsc.CB054) from anaerobically fermented bovine colostrum for its in vitro probiotic potential. We performed a series of in vitro tests to verify the probiotic capacity and safety of the bacterial isolate. These tests included the evaluat...
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Universidade Federal de Santa Maria
2025-04-01
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| Series: | Ciência Rural |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782025000600451&lng=en&tlng=en |
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| author | Carolina Litchina Brasil Júlia de Souza Silveira Juliana Marques Vitória Sequeira Gonçalves Caroline Quintana Braga Mara Helena Saalfeld Fábio Raphael Paschoti Brhun Fábio Pereira Leivas Leite Sônia de Avila Botton Daniela Isabel Brayer Pereira |
| author_facet | Carolina Litchina Brasil Júlia de Souza Silveira Juliana Marques Vitória Sequeira Gonçalves Caroline Quintana Braga Mara Helena Saalfeld Fábio Raphael Paschoti Brhun Fábio Pereira Leivas Leite Sônia de Avila Botton Daniela Isabel Brayer Pereira |
| author_sort | Carolina Litchina Brasil |
| collection | DOAJ |
| description | ABSTRACT: This study evaluated Lacticaseibacillus casei CB054 (Lsc.CB054) from anaerobically fermented bovine colostrum for its in vitro probiotic potential. We performed a series of in vitro tests to verify the probiotic capacity and safety of the bacterial isolate. These tests included the evaluation of hemolytic capacity, cytotoxicity, antimicrobial susceptibility, tolerance to adverse conditions, survival in the gastrointestinal tract, antagonistic activity against pathogenic bacteria, and auto- and co-aggregation abilities. The results demonstrated no hemolytic activity, and the microorganism did not exert a cytotoxic effect on Madin-Darby Bovine Kidney cells. It was observed that Lsc.CB054 was susceptible to nine (9/10) of the antimicrobials tested, showing resistance only to sulfonamide. When subjected to various acidic conditions, bile salts, and phenol, the survival rate of Lsc.CB054 was ≥ 8 log CFU/mL. In simulations of the intestinal tract, the presence of food with bile did not affect the multiplication of the bacterium; however, the duration of exposure impacted bacterial multiplication. Conversely, no viable cells of Lsc.CB054 were observed after 30 minutes of exposure to the simulated gastric tract without food. The isolate displayed antagonistic activity against Salmonella Typhimurium (18.5 mm), Staphylococcus aureus (23.5 mm), Listeria monocytogenes (15.5 mm), and Escherichia coli (18.0 mm), and exhibited auto-aggregation (> 75%) and co-aggregation with L. monocytogenes (> 79%). According to the results of this in vitro study, the probiotic potential of Lsc.CB054 is revealed. |
| format | Article |
| id | doaj-art-8415d70c9d7d47f0aa5a29550a6b51b3 |
| institution | OA Journals |
| issn | 1678-4596 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Universidade Federal de Santa Maria |
| record_format | Article |
| series | Ciência Rural |
| spelling | doaj-art-8415d70c9d7d47f0aa5a29550a6b51b32025-08-20T02:08:46ZengUniversidade Federal de Santa MariaCiência Rural1678-45962025-04-0155610.1590/0103-8478cr20240347In vitro probiotic properties of Lacticaseibacillus casei CB054 from anaerobically fermented bovine colostrumsCarolina Litchina Brasilhttps://orcid.org/0000-0002-2965-5014Júlia de Souza SilveiraJuliana MarquesVitória Sequeira Gonçalveshttps://orcid.org/0000-0003-1639-2494Caroline Quintana Bragahttps://orcid.org/0000-0003-1481-9829Mara Helena SaalfeldFábio Raphael Paschoti Brhunhttps://orcid.org/0000-0002-4191-965XFábio Pereira Leivas LeiteSônia de Avila Bottonhttps://orcid.org/0000-0002-8855-9217Daniela Isabel Brayer Pereirahttps://orcid.org/0000-0003-4374-677XABSTRACT: This study evaluated Lacticaseibacillus casei CB054 (Lsc.CB054) from anaerobically fermented bovine colostrum for its in vitro probiotic potential. We performed a series of in vitro tests to verify the probiotic capacity and safety of the bacterial isolate. These tests included the evaluation of hemolytic capacity, cytotoxicity, antimicrobial susceptibility, tolerance to adverse conditions, survival in the gastrointestinal tract, antagonistic activity against pathogenic bacteria, and auto- and co-aggregation abilities. The results demonstrated no hemolytic activity, and the microorganism did not exert a cytotoxic effect on Madin-Darby Bovine Kidney cells. It was observed that Lsc.CB054 was susceptible to nine (9/10) of the antimicrobials tested, showing resistance only to sulfonamide. When subjected to various acidic conditions, bile salts, and phenol, the survival rate of Lsc.CB054 was ≥ 8 log CFU/mL. In simulations of the intestinal tract, the presence of food with bile did not affect the multiplication of the bacterium; however, the duration of exposure impacted bacterial multiplication. Conversely, no viable cells of Lsc.CB054 were observed after 30 minutes of exposure to the simulated gastric tract without food. The isolate displayed antagonistic activity against Salmonella Typhimurium (18.5 mm), Staphylococcus aureus (23.5 mm), Listeria monocytogenes (15.5 mm), and Escherichia coli (18.0 mm), and exhibited auto-aggregation (> 75%) and co-aggregation with L. monocytogenes (> 79%). According to the results of this in vitro study, the probiotic potential of Lsc.CB054 is revealed.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782025000600451&lng=en&tlng=enlactic acid bacteriaantagonistic activityauto-aggregationco-aggregationtolerance |
| spellingShingle | Carolina Litchina Brasil Júlia de Souza Silveira Juliana Marques Vitória Sequeira Gonçalves Caroline Quintana Braga Mara Helena Saalfeld Fábio Raphael Paschoti Brhun Fábio Pereira Leivas Leite Sônia de Avila Botton Daniela Isabel Brayer Pereira In vitro probiotic properties of Lacticaseibacillus casei CB054 from anaerobically fermented bovine colostrums Ciência Rural lactic acid bacteria antagonistic activity auto-aggregation co-aggregation tolerance |
| title | In vitro probiotic properties of Lacticaseibacillus casei CB054 from anaerobically fermented bovine colostrums |
| title_full | In vitro probiotic properties of Lacticaseibacillus casei CB054 from anaerobically fermented bovine colostrums |
| title_fullStr | In vitro probiotic properties of Lacticaseibacillus casei CB054 from anaerobically fermented bovine colostrums |
| title_full_unstemmed | In vitro probiotic properties of Lacticaseibacillus casei CB054 from anaerobically fermented bovine colostrums |
| title_short | In vitro probiotic properties of Lacticaseibacillus casei CB054 from anaerobically fermented bovine colostrums |
| title_sort | in vitro probiotic properties of lacticaseibacillus casei cb054 from anaerobically fermented bovine colostrums |
| topic | lactic acid bacteria antagonistic activity auto-aggregation co-aggregation tolerance |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782025000600451&lng=en&tlng=en |
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