Accelerated Storage Testing of Vacuum-Dried <i>Lactobacillus acidophilus TISTR 1338</i> for Feed
Lactic acid bacteria (LAB) are widely used to produce various food products, adding flavor, texture, and health benefits. The bacteria are commonly grown on expensive nutrients like glucose, sucrose, and yeast extracts, which makes them commercially unappealing. In the current study, <i>Lactob...
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2024-12-01
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| author | Apichet Muenaram Bhundit Innawong Imrana Niaz Sultan Muhammad Waseem Khan Hamza Ghilzai Afrasiab Khan Tareen Pramuk Parakulsuksatid |
| author_facet | Apichet Muenaram Bhundit Innawong Imrana Niaz Sultan Muhammad Waseem Khan Hamza Ghilzai Afrasiab Khan Tareen Pramuk Parakulsuksatid |
| author_sort | Apichet Muenaram |
| collection | DOAJ |
| description | Lactic acid bacteria (LAB) are widely used to produce various food products, adding flavor, texture, and health benefits. The bacteria are commonly grown on expensive nutrients like glucose, sucrose, and yeast extracts, which makes them commercially unappealing. In the current study, <i>Lactobacillus acidophilus TISTR 1338</i> culture was studied using spent cell yeast as a nitrogen source and molasses as a carbon source. The drying process used to create starter cultures of <i>Lactobacillus acidophilus TISTR 1338</i> was vacuum drying. After vacuum drying, this bacterium had a survival rate of 8.08 log CFU/g. The dried strain survived for four months at 37 °C. With wasted cells at 0.5%, molasses concentration at 11% at 2.14 109 CFU/mL at 22 h, precise growth rate at 0.39 h<sup>−1</sup>, and yield cell mass at 1.67 1011 CFU/g sugar, yeast produced the maximum cell mass. The lower viability of the tested strain was induced by a higher temperature during this prolonged storage. Meanwhile, dehydrated starter culture was subjected to accelerated storage testing at 50, 60, and 70 °C. To determine the vacuum-dried <i>Lactobacillus acidophilus TISTR 1338</i>′s long-term storage viability, a temperature-dependent prophecy model was created. Molasses and spent cell yeast serve as promising carbon and nitrogen sources when optimized conditions are employed. The study also suggests that vacuum drying is a promising method for producing dried cells suitable for non-refrigerated storage conditions. |
| format | Article |
| id | doaj-art-82d81db988d1468dbaf57a5bee2fb07c |
| institution | Kabale University |
| issn | 2036-7481 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microbiology Research |
| spelling | doaj-art-82d81db988d1468dbaf57a5bee2fb07c2025-01-24T13:41:42ZengMDPI AGMicrobiology Research2036-74812024-12-01161610.3390/microbiolres16010006Accelerated Storage Testing of Vacuum-Dried <i>Lactobacillus acidophilus TISTR 1338</i> for FeedApichet Muenaram0Bhundit Innawong1Imrana Niaz Sultan2Muhammad Waseem Khan3Hamza Ghilzai4Afrasiab Khan Tareen5Pramuk Parakulsuksatid6Department of Biotechnology, Faculty of Agro-Industry, Kasetsart University, Bangkok 10900, ThailandInstitute for Local Economic Foundation, Kanchanaburi 71000, ThailandDepartment of Biotechnology, Faculty of Agro-Industry, Kasetsart University, Bangkok 10900, ThailandDepartment of Biotechnology, Faculty of Life Sciences, Balochistan University of Information Technology Engineering & Management Sciences, Quetta 87300, PakistanDepartment of Biotechnology, Faculty of Life Sciences, Balochistan University of Information Technology Engineering & Management Sciences, Quetta 87300, PakistanDepartment of Biotechnology, Faculty of Agro-Industry, Kasetsart University, Bangkok 10900, ThailandDepartment of Biotechnology, Faculty of Agro-Industry, Kasetsart University, Bangkok 10900, ThailandLactic acid bacteria (LAB) are widely used to produce various food products, adding flavor, texture, and health benefits. The bacteria are commonly grown on expensive nutrients like glucose, sucrose, and yeast extracts, which makes them commercially unappealing. In the current study, <i>Lactobacillus acidophilus TISTR 1338</i> culture was studied using spent cell yeast as a nitrogen source and molasses as a carbon source. The drying process used to create starter cultures of <i>Lactobacillus acidophilus TISTR 1338</i> was vacuum drying. After vacuum drying, this bacterium had a survival rate of 8.08 log CFU/g. The dried strain survived for four months at 37 °C. With wasted cells at 0.5%, molasses concentration at 11% at 2.14 109 CFU/mL at 22 h, precise growth rate at 0.39 h<sup>−1</sup>, and yield cell mass at 1.67 1011 CFU/g sugar, yeast produced the maximum cell mass. The lower viability of the tested strain was induced by a higher temperature during this prolonged storage. Meanwhile, dehydrated starter culture was subjected to accelerated storage testing at 50, 60, and 70 °C. To determine the vacuum-dried <i>Lactobacillus acidophilus TISTR 1338</i>′s long-term storage viability, a temperature-dependent prophecy model was created. Molasses and spent cell yeast serve as promising carbon and nitrogen sources when optimized conditions are employed. The study also suggests that vacuum drying is a promising method for producing dried cells suitable for non-refrigerated storage conditions.https://www.mdpi.com/2036-7481/16/1/6accelerated storage testingArrhenius theoryshelf lifevacuum drying<i>Lactobacillus acidophilus TISTR 1338</i> |
| spellingShingle | Apichet Muenaram Bhundit Innawong Imrana Niaz Sultan Muhammad Waseem Khan Hamza Ghilzai Afrasiab Khan Tareen Pramuk Parakulsuksatid Accelerated Storage Testing of Vacuum-Dried <i>Lactobacillus acidophilus TISTR 1338</i> for Feed Microbiology Research accelerated storage testing Arrhenius theory shelf life vacuum drying <i>Lactobacillus acidophilus TISTR 1338</i> |
| title | Accelerated Storage Testing of Vacuum-Dried <i>Lactobacillus acidophilus TISTR 1338</i> for Feed |
| title_full | Accelerated Storage Testing of Vacuum-Dried <i>Lactobacillus acidophilus TISTR 1338</i> for Feed |
| title_fullStr | Accelerated Storage Testing of Vacuum-Dried <i>Lactobacillus acidophilus TISTR 1338</i> for Feed |
| title_full_unstemmed | Accelerated Storage Testing of Vacuum-Dried <i>Lactobacillus acidophilus TISTR 1338</i> for Feed |
| title_short | Accelerated Storage Testing of Vacuum-Dried <i>Lactobacillus acidophilus TISTR 1338</i> for Feed |
| title_sort | accelerated storage testing of vacuum dried i lactobacillus acidophilus tistr 1338 i for feed |
| topic | accelerated storage testing Arrhenius theory shelf life vacuum drying <i>Lactobacillus acidophilus TISTR 1338</i> |
| url | https://www.mdpi.com/2036-7481/16/1/6 |
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