Induction of Macrophage M2b/c Polarization by Adipose Tissue-Derived Mesenchymal Stem Cells
Background. Adipose-derived mesenchymal stem cells (ADMSCs) can promote healing and inhibit inflammation/immune response in local tissues, while the detailed mechanism remains unknown. Results. ADMSCs and peritoneal macrophages were collected from C57BL/6 mice. The culture medium (CM) from ADMSCs (2...
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| Format: | Article |
| Language: | English |
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Wiley
2019-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2019/7059680 |
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| author | Meng Sun Linxiao Sun Chongchu Huang Bi-cheng Chen Zhenxu Zhou |
| author_facet | Meng Sun Linxiao Sun Chongchu Huang Bi-cheng Chen Zhenxu Zhou |
| author_sort | Meng Sun |
| collection | DOAJ |
| description | Background. Adipose-derived mesenchymal stem cells (ADMSCs) can promote healing and inhibit inflammation/immune response in local tissues, while the detailed mechanism remains unknown. Results. ADMSCs and peritoneal macrophages were collected from C57BL/6 mice. The culture medium (CM) from ADMSCs (24 hours cultured) was collected. The CM was added to the Mφ culture system with lipopolysaccharide (LPS) or IL-4/IL-13 or blank. And those Mφ cultures without adding CM were used as controls. A series of classification markers and signaling pathways for Mφ polarization were detected by using flow cytometry, RT-PCR, and western blotting. Furthermore, the cell viability of all the groups was detected by CCK8 assay. After CM induction in different groups, M1-Mφ markers and M2a-Mφ were decreased; however, M2b/c-Mφ markers increased. STAT3/SOCS3 and STAT6/IRF4 were suppressed in all 3 CM-treated groups. Moreover, the cell viability of all 3 groups which were induced by CM significantly increased as compared to that of the control groups without adding CM. Conclusion. ADMSCs can induce nonactivated macrophage and M1-Mφ into M2b/c-Mφ. Downregulation of the STAT3 and STAT6 pathway may involve in this process. This data shows that the anti-inflammatory role of ADMSC in local tissues may be partly due to their effect on Mφ to M2b/c-Mφ. |
| format | Article |
| id | doaj-art-82530b4be8af4ba1ae5569ee57055b0f |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-82530b4be8af4ba1ae5569ee57055b0f2025-02-03T06:08:09ZengWileyJournal of Immunology Research2314-88612314-71562019-01-01201910.1155/2019/70596807059680Induction of Macrophage M2b/c Polarization by Adipose Tissue-Derived Mesenchymal Stem CellsMeng Sun0Linxiao Sun1Chongchu Huang2Bi-cheng Chen3Zhenxu Zhou4Key Laboratory of Diagnosis and Treatment of Severe Hepato-Pancreatic Diseases of Zhejiang Province, Zhejiang Provincial Top Key Discipline in Surgery, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, ChinaKey Laboratory of Diagnosis and Treatment of Severe Hepato-Pancreatic Diseases of Zhejiang Province, Zhejiang Provincial Top Key Discipline in Surgery, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, ChinaKey Laboratory of Diagnosis and Treatment of Severe Hepato-Pancreatic Diseases of Zhejiang Province, Zhejiang Provincial Top Key Discipline in Surgery, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, ChinaKey Laboratory of Diagnosis and Treatment of Severe Hepato-Pancreatic Diseases of Zhejiang Province, Zhejiang Provincial Top Key Discipline in Surgery, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, ChinaKey Laboratory of Diagnosis and Treatment of Severe Hepato-Pancreatic Diseases of Zhejiang Province, Zhejiang Provincial Top Key Discipline in Surgery, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, ChinaBackground. Adipose-derived mesenchymal stem cells (ADMSCs) can promote healing and inhibit inflammation/immune response in local tissues, while the detailed mechanism remains unknown. Results. ADMSCs and peritoneal macrophages were collected from C57BL/6 mice. The culture medium (CM) from ADMSCs (24 hours cultured) was collected. The CM was added to the Mφ culture system with lipopolysaccharide (LPS) or IL-4/IL-13 or blank. And those Mφ cultures without adding CM were used as controls. A series of classification markers and signaling pathways for Mφ polarization were detected by using flow cytometry, RT-PCR, and western blotting. Furthermore, the cell viability of all the groups was detected by CCK8 assay. After CM induction in different groups, M1-Mφ markers and M2a-Mφ were decreased; however, M2b/c-Mφ markers increased. STAT3/SOCS3 and STAT6/IRF4 were suppressed in all 3 CM-treated groups. Moreover, the cell viability of all 3 groups which were induced by CM significantly increased as compared to that of the control groups without adding CM. Conclusion. ADMSCs can induce nonactivated macrophage and M1-Mφ into M2b/c-Mφ. Downregulation of the STAT3 and STAT6 pathway may involve in this process. This data shows that the anti-inflammatory role of ADMSC in local tissues may be partly due to their effect on Mφ to M2b/c-Mφ.http://dx.doi.org/10.1155/2019/7059680 |
| spellingShingle | Meng Sun Linxiao Sun Chongchu Huang Bi-cheng Chen Zhenxu Zhou Induction of Macrophage M2b/c Polarization by Adipose Tissue-Derived Mesenchymal Stem Cells Journal of Immunology Research |
| title | Induction of Macrophage M2b/c Polarization by Adipose Tissue-Derived Mesenchymal Stem Cells |
| title_full | Induction of Macrophage M2b/c Polarization by Adipose Tissue-Derived Mesenchymal Stem Cells |
| title_fullStr | Induction of Macrophage M2b/c Polarization by Adipose Tissue-Derived Mesenchymal Stem Cells |
| title_full_unstemmed | Induction of Macrophage M2b/c Polarization by Adipose Tissue-Derived Mesenchymal Stem Cells |
| title_short | Induction of Macrophage M2b/c Polarization by Adipose Tissue-Derived Mesenchymal Stem Cells |
| title_sort | induction of macrophage m2b c polarization by adipose tissue derived mesenchymal stem cells |
| url | http://dx.doi.org/10.1155/2019/7059680 |
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