Expressed Sequence Tags Analysis and Design of Simple Sequence Repeats Markers from a Full-Length cDNA Library in Perilla frutescens (L.)
Perilla frutescens is valuable as a medicinal plant as well as a natural medicine and functional food. However, comparative genomics analyses of P. frutescens are limited due to a lack of gene annotations and characterization. A full-length cDNA library from P. frutescens leaves was constructed to i...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | International Journal of Genomics |
| Online Access: | http://dx.doi.org/10.1155/2015/679548 |
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| author | Eun Soo Seong Ji Hye Yoo Jae Hoo Choi Chang Heum Kim Mi Ran Jeon Byeong Ju Kang Jae Geun Lee Seon Kang Choi Bimal Kumar Ghimire Chang Yeon Yu |
| author_facet | Eun Soo Seong Ji Hye Yoo Jae Hoo Choi Chang Heum Kim Mi Ran Jeon Byeong Ju Kang Jae Geun Lee Seon Kang Choi Bimal Kumar Ghimire Chang Yeon Yu |
| author_sort | Eun Soo Seong |
| collection | DOAJ |
| description | Perilla frutescens is valuable as a medicinal plant as well as a natural medicine and functional food. However, comparative genomics analyses of P. frutescens are limited due to a lack of gene annotations and characterization. A full-length cDNA library from P. frutescens leaves was constructed to identify functional gene clusters and probable EST-SSR markers via analysis of 1,056 expressed sequence tags. Unigene assembly was performed using basic local alignment search tool (BLAST) homology searches and annotated Gene Ontology (GO). A total of 18 simple sequence repeats (SSRs) were designed as primer pairs. This study is the first to report comparative genomics and EST-SSR markers from P. frutescens will help gene discovery and provide an important source for functional genomics and molecular genetic research in this interesting medicinal plant. |
| format | Article |
| id | doaj-art-81eabcdef88b4ec9b656597e7fd5faa9 |
| institution | Kabale University |
| issn | 2314-436X 2314-4378 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Genomics |
| spelling | doaj-art-81eabcdef88b4ec9b656597e7fd5faa92025-02-03T06:13:59ZengWileyInternational Journal of Genomics2314-436X2314-43782015-01-01201510.1155/2015/679548679548Expressed Sequence Tags Analysis and Design of Simple Sequence Repeats Markers from a Full-Length cDNA Library in Perilla frutescens (L.)Eun Soo Seong0Ji Hye Yoo1Jae Hoo Choi2Chang Heum Kim3Mi Ran Jeon4Byeong Ju Kang5Jae Geun Lee6Seon Kang Choi7Bimal Kumar Ghimire8Chang Yeon Yu9Bioherb Research Institute, Kangwon National University, Chuncheon 200-701, Republic of KoreaDepartment of Bioconvergence Science and Technology, College of Agriculture and Life Sciences, Kangwon National University, Chuncheon 200-701, Republic of KoreaDepartment of Bioconvergence Science and Technology, College of Agriculture and Life Sciences, Kangwon National University, Chuncheon 200-701, Republic of KoreaDepartment of Bioconvergence Science and Technology, College of Agriculture and Life Sciences, Kangwon National University, Chuncheon 200-701, Republic of KoreaDepartment of Bioconvergence Science and Technology, College of Agriculture and Life Sciences, Kangwon National University, Chuncheon 200-701, Republic of KoreaDepartment of Bioconvergence Science and Technology, College of Agriculture and Life Sciences, Kangwon National University, Chuncheon 200-701, Republic of KoreaHwajin Cosmetics, Hongcheon 250-807, Republic of KoreaDepartment of Agricultural Life Sciences, Kangwon National University, Chuncheon 200-701, Republic of KoreaDepartment of Applied Bioscience, Konkuk University, Seoul 143-701, Republic of KoreaDepartment of Bioconvergence Science and Technology, College of Agriculture and Life Sciences, Kangwon National University, Chuncheon 200-701, Republic of KoreaPerilla frutescens is valuable as a medicinal plant as well as a natural medicine and functional food. However, comparative genomics analyses of P. frutescens are limited due to a lack of gene annotations and characterization. A full-length cDNA library from P. frutescens leaves was constructed to identify functional gene clusters and probable EST-SSR markers via analysis of 1,056 expressed sequence tags. Unigene assembly was performed using basic local alignment search tool (BLAST) homology searches and annotated Gene Ontology (GO). A total of 18 simple sequence repeats (SSRs) were designed as primer pairs. This study is the first to report comparative genomics and EST-SSR markers from P. frutescens will help gene discovery and provide an important source for functional genomics and molecular genetic research in this interesting medicinal plant.http://dx.doi.org/10.1155/2015/679548 |
| spellingShingle | Eun Soo Seong Ji Hye Yoo Jae Hoo Choi Chang Heum Kim Mi Ran Jeon Byeong Ju Kang Jae Geun Lee Seon Kang Choi Bimal Kumar Ghimire Chang Yeon Yu Expressed Sequence Tags Analysis and Design of Simple Sequence Repeats Markers from a Full-Length cDNA Library in Perilla frutescens (L.) International Journal of Genomics |
| title | Expressed Sequence Tags Analysis and Design of Simple Sequence Repeats Markers from a Full-Length cDNA Library in Perilla frutescens (L.) |
| title_full | Expressed Sequence Tags Analysis and Design of Simple Sequence Repeats Markers from a Full-Length cDNA Library in Perilla frutescens (L.) |
| title_fullStr | Expressed Sequence Tags Analysis and Design of Simple Sequence Repeats Markers from a Full-Length cDNA Library in Perilla frutescens (L.) |
| title_full_unstemmed | Expressed Sequence Tags Analysis and Design of Simple Sequence Repeats Markers from a Full-Length cDNA Library in Perilla frutescens (L.) |
| title_short | Expressed Sequence Tags Analysis and Design of Simple Sequence Repeats Markers from a Full-Length cDNA Library in Perilla frutescens (L.) |
| title_sort | expressed sequence tags analysis and design of simple sequence repeats markers from a full length cdna library in perilla frutescens l |
| url | http://dx.doi.org/10.1155/2015/679548 |
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