Screening for transcriptomic associations with Swine Inflammation and Necrosis Syndrome
Abstract Background The recently identified swine inflammation and necrosis syndrome (SINS) affects tail, ears, teats, coronary bands, claws and heels of affected individuals. The primarily endogenous syndrome is based on vasculitis, thrombosis, and intimal proliferation, involving defence cells, in...
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2025-01-01
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| Online Access: | https://doi.org/10.1186/s12917-024-04469-y |
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| author | Katharina Gerhards Sabrina Becker Josef Kuehling Mirjam Lechner Hermann Willems Robert Ringseis Gerald Reiner |
| author_facet | Katharina Gerhards Sabrina Becker Josef Kuehling Mirjam Lechner Hermann Willems Robert Ringseis Gerald Reiner |
| author_sort | Katharina Gerhards |
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| description | Abstract Background The recently identified swine inflammation and necrosis syndrome (SINS) affects tail, ears, teats, coronary bands, claws and heels of affected individuals. The primarily endogenous syndrome is based on vasculitis, thrombosis, and intimal proliferation, involving defence cells, interleukins, chemokines, and acute phase proteins and accompanied by alterations in clinical chemistry, metabolome, and liver transcriptome. The complexity of metabolic alterations and the influence of the boar led to hypothesize a polygenic architecture of SINS. This should be investigated by a transcriptome study. For this purpose, the three to five least affected (SINS-low) and most SINS affected (SINS-high) 3d-old piglets, each of three boars, a relatively SINS stable Duroc boar (DU), a relatively stable Pietrain boar (PI+) and a highly susceptible Pietrain boar (PI-) were selected from 27 litters of mixed semen to minimize environmental effects. Results A genome-wide expression experiment revealed a huge set of differentially expressed genes that are involved in vasculitis, inflammation and necrosis, keratinization and erythrocyte epitopes. Among them were CRP, GYPA, S100A12, and LIPK. The results confirm and complement previous studies to this topic. Conclusions The results confirm the outstanding importance of defence in the context of SINS. At the same time, for the first time, there is evidence for a direct involvement of the keratinisation capacity of the skin and various epitopes of the erythrocyte membrane, which seem to be associated with the severity of SINS. These genes could serve to clarify the pathogenesis of the syndrome and to develop diagnostic tools in future studies. |
| format | Article |
| id | doaj-art-81e4fc7765b94c9b86b6c7937c6127d3 |
| institution | Kabale University |
| issn | 1746-6148 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Veterinary Research |
| spelling | doaj-art-81e4fc7765b94c9b86b6c7937c6127d32025-01-19T12:27:14ZengBMCBMC Veterinary Research1746-61482025-01-0121111910.1186/s12917-024-04469-yScreening for transcriptomic associations with Swine Inflammation and Necrosis SyndromeKatharina Gerhards0Sabrina Becker1Josef Kuehling2Mirjam Lechner3Hermann Willems4Robert Ringseis5Gerald Reiner6Department of Veterinary Clinical Sciences, Clinic for Swine, Justus-Liebig-UniversityDepartment of Veterinary Clinical Sciences, Clinic for Swine, Justus-Liebig-UniversityDepartment of Veterinary Clinical Sciences, Clinic for Swine, Justus-Liebig-UniversityUEG HohenloheDepartment of Veterinary Clinical Sciences, Clinic for Swine, Justus-Liebig-UniversityInstitute of Animal Nutrition and Nutrition Physiology, Justus Liebig University GiessenDepartment of Veterinary Clinical Sciences, Clinic for Swine, Justus-Liebig-UniversityAbstract Background The recently identified swine inflammation and necrosis syndrome (SINS) affects tail, ears, teats, coronary bands, claws and heels of affected individuals. The primarily endogenous syndrome is based on vasculitis, thrombosis, and intimal proliferation, involving defence cells, interleukins, chemokines, and acute phase proteins and accompanied by alterations in clinical chemistry, metabolome, and liver transcriptome. The complexity of metabolic alterations and the influence of the boar led to hypothesize a polygenic architecture of SINS. This should be investigated by a transcriptome study. For this purpose, the three to five least affected (SINS-low) and most SINS affected (SINS-high) 3d-old piglets, each of three boars, a relatively SINS stable Duroc boar (DU), a relatively stable Pietrain boar (PI+) and a highly susceptible Pietrain boar (PI-) were selected from 27 litters of mixed semen to minimize environmental effects. Results A genome-wide expression experiment revealed a huge set of differentially expressed genes that are involved in vasculitis, inflammation and necrosis, keratinization and erythrocyte epitopes. Among them were CRP, GYPA, S100A12, and LIPK. The results confirm and complement previous studies to this topic. Conclusions The results confirm the outstanding importance of defence in the context of SINS. At the same time, for the first time, there is evidence for a direct involvement of the keratinisation capacity of the skin and various epitopes of the erythrocyte membrane, which seem to be associated with the severity of SINS. These genes could serve to clarify the pathogenesis of the syndrome and to develop diagnostic tools in future studies.https://doi.org/10.1186/s12917-024-04469-yInflammationNecrosisSwineAnimal welfareMetabolism |
| spellingShingle | Katharina Gerhards Sabrina Becker Josef Kuehling Mirjam Lechner Hermann Willems Robert Ringseis Gerald Reiner Screening for transcriptomic associations with Swine Inflammation and Necrosis Syndrome BMC Veterinary Research Inflammation Necrosis Swine Animal welfare Metabolism |
| title | Screening for transcriptomic associations with Swine Inflammation and Necrosis Syndrome |
| title_full | Screening for transcriptomic associations with Swine Inflammation and Necrosis Syndrome |
| title_fullStr | Screening for transcriptomic associations with Swine Inflammation and Necrosis Syndrome |
| title_full_unstemmed | Screening for transcriptomic associations with Swine Inflammation and Necrosis Syndrome |
| title_short | Screening for transcriptomic associations with Swine Inflammation and Necrosis Syndrome |
| title_sort | screening for transcriptomic associations with swine inflammation and necrosis syndrome |
| topic | Inflammation Necrosis Swine Animal welfare Metabolism |
| url | https://doi.org/10.1186/s12917-024-04469-y |
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