Overexpression of SFPQ Improves Cognition and Memory in AD Mice
Alzheimer’s disease (AD) is a complex neurodegenerative disorder with multifaceted pathogenesis, which has been extensively investigated, yet effective treatments remain lacking. Splicing factor proline and glutamine rich (SFPQ) is known to play a crucial role in neurodegenerative diseases, includin...
Saved in:
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2025-01-01
|
| Series: | Neural Plasticity |
| Online Access: | http://dx.doi.org/10.1155/np/3934591 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832088019858882560 |
|---|---|
| author | Jinshan Tie Hongxiang Wu Wei Liu Yuying Li Lu Li Suju Zhao Zhijiao Yuan Khan Mahmood Shaochun Chen Huidong Wu |
| author_facet | Jinshan Tie Hongxiang Wu Wei Liu Yuying Li Lu Li Suju Zhao Zhijiao Yuan Khan Mahmood Shaochun Chen Huidong Wu |
| author_sort | Jinshan Tie |
| collection | DOAJ |
| description | Alzheimer’s disease (AD) is a complex neurodegenerative disorder with multifaceted pathogenesis, which has been extensively investigated, yet effective treatments remain lacking. Splicing factor proline and glutamine rich (SFPQ) is known to play a crucial role in neurodegenerative diseases, including antioxidant-related functions and regulating gene expression within brain neurons. However, the specific role of SFPQ in AD pathology is not well understood. In this study, an AD mouse model was established through lateral ventricular injection of amyloid-beta1–42 (Aβ1–42). Subsequently, adeno-associated virus was administered to overexpress SFPQ in the hippocampus of AD mice. The results demonstrate that SFPQ overexpression improves recognition and memory in AD mice, while reducing AD-related marker proteins such as amyloid precursor protein (APP) and Tau. Additionally, synaptic and memory-associated proteins, as well as antioxidant proteins like glutathione S-transferase (GST) and heme oxygenase-1 (HO-1), were upregulated. The ratio of antiapoptotic protein Bcl-2 to proapoptotic protein Bax also increased. Furthermore, phosphorylated phosphoinositide 3-kinase (p-PI3K)/PI3K and phosphorylated protein kinase B (p-AKT)/AKT ratios were elevated, indicating activation of the PI3K/AKT signaling pathway. These findings suggest that SFPQ may serve as a promising molecular target for the prevention and treatment of AD. |
| format | Article |
| id | doaj-art-8097a13c8ec6437b8575aab9745a7685 |
| institution | Kabale University |
| issn | 1687-5443 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Neural Plasticity |
| spelling | doaj-art-8097a13c8ec6437b8575aab9745a76852025-02-06T00:00:02ZengWileyNeural Plasticity1687-54432025-01-01202510.1155/np/3934591Overexpression of SFPQ Improves Cognition and Memory in AD MiceJinshan Tie0Hongxiang Wu1Wei Liu2Yuying Li3Lu Li4Suju Zhao5Zhijiao Yuan6Khan Mahmood7Shaochun Chen8Huidong Wu9School of RehabilitationSchool of RehabilitationSchool of RehabilitationSchool of RehabilitationSchool of RehabilitationSchool of RehabilitationSchool of RehabilitationSchool of RehabilitationSchool of RehabilitationSchool of RehabilitationAlzheimer’s disease (AD) is a complex neurodegenerative disorder with multifaceted pathogenesis, which has been extensively investigated, yet effective treatments remain lacking. Splicing factor proline and glutamine rich (SFPQ) is known to play a crucial role in neurodegenerative diseases, including antioxidant-related functions and regulating gene expression within brain neurons. However, the specific role of SFPQ in AD pathology is not well understood. In this study, an AD mouse model was established through lateral ventricular injection of amyloid-beta1–42 (Aβ1–42). Subsequently, adeno-associated virus was administered to overexpress SFPQ in the hippocampus of AD mice. The results demonstrate that SFPQ overexpression improves recognition and memory in AD mice, while reducing AD-related marker proteins such as amyloid precursor protein (APP) and Tau. Additionally, synaptic and memory-associated proteins, as well as antioxidant proteins like glutathione S-transferase (GST) and heme oxygenase-1 (HO-1), were upregulated. The ratio of antiapoptotic protein Bcl-2 to proapoptotic protein Bax also increased. Furthermore, phosphorylated phosphoinositide 3-kinase (p-PI3K)/PI3K and phosphorylated protein kinase B (p-AKT)/AKT ratios were elevated, indicating activation of the PI3K/AKT signaling pathway. These findings suggest that SFPQ may serve as a promising molecular target for the prevention and treatment of AD.http://dx.doi.org/10.1155/np/3934591 |
| spellingShingle | Jinshan Tie Hongxiang Wu Wei Liu Yuying Li Lu Li Suju Zhao Zhijiao Yuan Khan Mahmood Shaochun Chen Huidong Wu Overexpression of SFPQ Improves Cognition and Memory in AD Mice Neural Plasticity |
| title | Overexpression of SFPQ Improves Cognition and Memory in AD Mice |
| title_full | Overexpression of SFPQ Improves Cognition and Memory in AD Mice |
| title_fullStr | Overexpression of SFPQ Improves Cognition and Memory in AD Mice |
| title_full_unstemmed | Overexpression of SFPQ Improves Cognition and Memory in AD Mice |
| title_short | Overexpression of SFPQ Improves Cognition and Memory in AD Mice |
| title_sort | overexpression of sfpq improves cognition and memory in ad mice |
| url | http://dx.doi.org/10.1155/np/3934591 |
| work_keys_str_mv | AT jinshantie overexpressionofsfpqimprovescognitionandmemoryinadmice AT hongxiangwu overexpressionofsfpqimprovescognitionandmemoryinadmice AT weiliu overexpressionofsfpqimprovescognitionandmemoryinadmice AT yuyingli overexpressionofsfpqimprovescognitionandmemoryinadmice AT luli overexpressionofsfpqimprovescognitionandmemoryinadmice AT sujuzhao overexpressionofsfpqimprovescognitionandmemoryinadmice AT zhijiaoyuan overexpressionofsfpqimprovescognitionandmemoryinadmice AT khanmahmood overexpressionofsfpqimprovescognitionandmemoryinadmice AT shaochunchen overexpressionofsfpqimprovescognitionandmemoryinadmice AT huidongwu overexpressionofsfpqimprovescognitionandmemoryinadmice |