Effect of Uncaria rhynchophylla against Thioacetamide-Induced Acute Liver Injury in Rat
Both oxidative stress (OS) and inflammation are two fundamental pathological processes of acute liver injury (ALI). The current work is to investigate the effect and possible mechanism of Uncaria rhynchophylla (UR) on thioacetamide- (TAA-) induced ALI in rats. UR (100 and 200 mg/kg) was orally admin...
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| Format: | Article |
| Language: | English |
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Wiley
2021-01-01
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| Series: | Canadian Journal of Gastroenterology and Hepatology |
| Online Access: | http://dx.doi.org/10.1155/2021/5581816 |
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| author | Mi-Rae Shin Min Ju Kim Jin A Lee Seong-Soo Roh |
| author_facet | Mi-Rae Shin Min Ju Kim Jin A Lee Seong-Soo Roh |
| author_sort | Mi-Rae Shin |
| collection | DOAJ |
| description | Both oxidative stress (OS) and inflammation are two fundamental pathological processes of acute liver injury (ALI). The current work is to investigate the effect and possible mechanism of Uncaria rhynchophylla (UR) on thioacetamide- (TAA-) induced ALI in rats. UR (100 and 200 mg/kg) was orally administrated with TAA (200 mg/kg of bodyweight, intraperitoneal injection) for 3 consecutive days. ALI was confirmed using histological examination and the factors associated with OS and liver function activity measured in serum. Moreover, expressions of inflammation and collagen-related proteins were measured by the Western blot analysis. Myeloperoxidase (MPO), which mediates OS in the ALI control group, was manifested by a significant rise compared with the normal group. UR significantly reduced AST, ALT, and ammonia levels in serum. The nuclear factor-κB (NF-κB) activation induced by TAA led to increase both inflammatory mediators and cytokines. Whereas, UR administration remarkably suppressed such an overexpression. UR supplementation improved matrix metalloproteinases (MMPs) such as MMP-1, -2, and -8. In contrast, tissue inhibitors of metalloproteinases- (TIMP-) 1 level increased significantly by UR treatment. In addition, the histopathological analysis showed that the liver tissue lesions were improved obviously by UR treatment. UR may ameliorate the effects of TAA-induced ALI in rats by suppressing both OS through MPO activation and proinflammatory factors through NF-κB activation. In conclusion, UR exhibited a potent hepatoprotective effect on ALI through the suppression of OS. |
| format | Article |
| id | doaj-art-803b9e0568f0407e81d9367a246db15b |
| institution | Kabale University |
| issn | 2291-2789 2291-2797 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
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| series | Canadian Journal of Gastroenterology and Hepatology |
| spelling | doaj-art-803b9e0568f0407e81d9367a246db15b2025-02-03T06:08:33ZengWileyCanadian Journal of Gastroenterology and Hepatology2291-27892291-27972021-01-01202110.1155/2021/55818165581816Effect of Uncaria rhynchophylla against Thioacetamide-Induced Acute Liver Injury in RatMi-Rae Shin0Min Ju Kim1Jin A Lee2Seong-Soo Roh3Department of Herbology, Korean Medicine of College, Daegu Haany University, Deagu 42158, Republic of KoreaDepartment of Herbology, Korean Medicine of College, Daegu Haany University, Deagu 42158, Republic of KoreaDepartment of Herbology, Korean Medicine of College, Daegu Haany University, Deagu 42158, Republic of KoreaDepartment of Herbology, Korean Medicine of College, Daegu Haany University, Deagu 42158, Republic of KoreaBoth oxidative stress (OS) and inflammation are two fundamental pathological processes of acute liver injury (ALI). The current work is to investigate the effect and possible mechanism of Uncaria rhynchophylla (UR) on thioacetamide- (TAA-) induced ALI in rats. UR (100 and 200 mg/kg) was orally administrated with TAA (200 mg/kg of bodyweight, intraperitoneal injection) for 3 consecutive days. ALI was confirmed using histological examination and the factors associated with OS and liver function activity measured in serum. Moreover, expressions of inflammation and collagen-related proteins were measured by the Western blot analysis. Myeloperoxidase (MPO), which mediates OS in the ALI control group, was manifested by a significant rise compared with the normal group. UR significantly reduced AST, ALT, and ammonia levels in serum. The nuclear factor-κB (NF-κB) activation induced by TAA led to increase both inflammatory mediators and cytokines. Whereas, UR administration remarkably suppressed such an overexpression. UR supplementation improved matrix metalloproteinases (MMPs) such as MMP-1, -2, and -8. In contrast, tissue inhibitors of metalloproteinases- (TIMP-) 1 level increased significantly by UR treatment. In addition, the histopathological analysis showed that the liver tissue lesions were improved obviously by UR treatment. UR may ameliorate the effects of TAA-induced ALI in rats by suppressing both OS through MPO activation and proinflammatory factors through NF-κB activation. In conclusion, UR exhibited a potent hepatoprotective effect on ALI through the suppression of OS.http://dx.doi.org/10.1155/2021/5581816 |
| spellingShingle | Mi-Rae Shin Min Ju Kim Jin A Lee Seong-Soo Roh Effect of Uncaria rhynchophylla against Thioacetamide-Induced Acute Liver Injury in Rat Canadian Journal of Gastroenterology and Hepatology |
| title | Effect of Uncaria rhynchophylla against Thioacetamide-Induced Acute Liver Injury in Rat |
| title_full | Effect of Uncaria rhynchophylla against Thioacetamide-Induced Acute Liver Injury in Rat |
| title_fullStr | Effect of Uncaria rhynchophylla against Thioacetamide-Induced Acute Liver Injury in Rat |
| title_full_unstemmed | Effect of Uncaria rhynchophylla against Thioacetamide-Induced Acute Liver Injury in Rat |
| title_short | Effect of Uncaria rhynchophylla against Thioacetamide-Induced Acute Liver Injury in Rat |
| title_sort | effect of uncaria rhynchophylla against thioacetamide induced acute liver injury in rat |
| url | http://dx.doi.org/10.1155/2021/5581816 |
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