携带人胰岛素样生长因子-1重组腺病拆的构建和鉴定
［目的 l 采用Cre-LoxP同源重组系统构建并鉴定携带人胰岛素样生长因子-1 (human insulin-like growth factor-I, hIGF-1)目的基因的复制缺陷重组腺病毒，为椎间盘退变的h!GF-1基因治疗奠定基础。［方法】 PCR合成h!GF-1基因，用pMD18-T载体克隆h!GF-1目的基因，酶切、测序并进行NCBI BLAST相似性在线分析。将pMD18-T- hIGF-1和pDNR-lr质粒分别行EcoR I和BamH I双酶切，DNA连接酶连接双酶切产物，转化感受态大肠杆菌DH5ct,合成中间载体pDNR-hIGF-1, 酶切鉴定。Cre-loxP系统介...
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| Format: | Article |
| Language: | zho |
| Published: |
Editorial Office of Journal of Sun Yat-sen University
2004-01-01
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| Series: | Zhongshan Daxue xuebao. Yixue kexue ban |
| Online Access: | http://xuebaoyx.sysu.edu.cn/zh/article/43585600/ |
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