Multifunctional Thioredoxin-Like Protein from the Gastrointestinal Parasitic Nematodes Strongyloides ratti and Trichuris suis Affects Mucosal Homeostasis
The cellular redox state is important for the regulation of multiple functions and is essential for the maintenance of cellular homeostasis and antioxidant defense. In the excretory/secretory (E/S) products of Strongyloides ratti and Trichuris suis sequences for thioredoxin (Trx) and Trx-like protei...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Journal of Parasitology Research |
| Online Access: | http://dx.doi.org/10.1155/2016/8421597 |
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| author | Dana Ditgen Emmanuela M. Anandarajah Jan Hansmann Dominic Winter Guido Schramm Klaus D. Erttmann Eva Liebau Norbert W. Brattig |
| author_facet | Dana Ditgen Emmanuela M. Anandarajah Jan Hansmann Dominic Winter Guido Schramm Klaus D. Erttmann Eva Liebau Norbert W. Brattig |
| author_sort | Dana Ditgen |
| collection | DOAJ |
| description | The cellular redox state is important for the regulation of multiple functions and is essential for the maintenance of cellular homeostasis and antioxidant defense. In the excretory/secretory (E/S) products of Strongyloides ratti and Trichuris suis sequences for thioredoxin (Trx) and Trx-like protein (Trx-lp) were identified. To characterize the antioxidant Trx-lp and its interaction with the parasite’s mucosal habitat, S. ratti and T. suis Trx-lps were cloned and recombinantly expressed. The primary antioxidative activity was assured by reduction of insulin and IgM. Further analysis applying an in vitro mucosal 3D-cell culture model revealed that the secreted Trx-lps were able to bind to monocytic and intestinal epithelial cells and induce the time-dependent release of cytokines such as TNF-α, IL-22, and TSLP. In addition, the redox proteins also possessed chemotactic activity for monocytic THP-1 cells and fostered epithelial wound healing activity. These results confirm that the parasite-secreted Trx-lps are multifunctional proteins that can affect the host intestinal mucosa. |
| format | Article |
| id | doaj-art-7f91ac791dd040d2b88fe898faa01b5c |
| institution | Kabale University |
| issn | 2090-0023 2090-0031 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Parasitology Research |
| spelling | doaj-art-7f91ac791dd040d2b88fe898faa01b5c2025-02-03T01:22:16ZengWileyJournal of Parasitology Research2090-00232090-00312016-01-01201610.1155/2016/84215978421597Multifunctional Thioredoxin-Like Protein from the Gastrointestinal Parasitic Nematodes Strongyloides ratti and Trichuris suis Affects Mucosal HomeostasisDana Ditgen0Emmanuela M. Anandarajah1Jan Hansmann2Dominic Winter3Guido Schramm4Klaus D. Erttmann5Eva Liebau6Norbert W. Brattig7Department of Molecular Physiology, Westfälische Wilhelms-University, Münster, GermanyDepartment of Molecular Physiology, Westfälische Wilhelms-University, Münster, GermanyDepartment of Tissue Engineering and Regenerative Medicine (TERM), University of Würzburg, GermanyInstitute for Biochemistry and Molecular Biology, University of Bonn, Bonn, GermanyOvamed GmbH, Hamburg, GermanyDepartment of Molecular Medicine, Bernhard Nocht Institute for Tropical Medicine, Hamburg, GermanyDepartment of Molecular Physiology, Westfälische Wilhelms-University, Münster, GermanyDepartment of Molecular Medicine, Bernhard Nocht Institute for Tropical Medicine, Hamburg, GermanyThe cellular redox state is important for the regulation of multiple functions and is essential for the maintenance of cellular homeostasis and antioxidant defense. In the excretory/secretory (E/S) products of Strongyloides ratti and Trichuris suis sequences for thioredoxin (Trx) and Trx-like protein (Trx-lp) were identified. To characterize the antioxidant Trx-lp and its interaction with the parasite’s mucosal habitat, S. ratti and T. suis Trx-lps were cloned and recombinantly expressed. The primary antioxidative activity was assured by reduction of insulin and IgM. Further analysis applying an in vitro mucosal 3D-cell culture model revealed that the secreted Trx-lps were able to bind to monocytic and intestinal epithelial cells and induce the time-dependent release of cytokines such as TNF-α, IL-22, and TSLP. In addition, the redox proteins also possessed chemotactic activity for monocytic THP-1 cells and fostered epithelial wound healing activity. These results confirm that the parasite-secreted Trx-lps are multifunctional proteins that can affect the host intestinal mucosa.http://dx.doi.org/10.1155/2016/8421597 |
| spellingShingle | Dana Ditgen Emmanuela M. Anandarajah Jan Hansmann Dominic Winter Guido Schramm Klaus D. Erttmann Eva Liebau Norbert W. Brattig Multifunctional Thioredoxin-Like Protein from the Gastrointestinal Parasitic Nematodes Strongyloides ratti and Trichuris suis Affects Mucosal Homeostasis Journal of Parasitology Research |
| title | Multifunctional Thioredoxin-Like Protein from the Gastrointestinal Parasitic Nematodes Strongyloides ratti and Trichuris suis Affects Mucosal Homeostasis |
| title_full | Multifunctional Thioredoxin-Like Protein from the Gastrointestinal Parasitic Nematodes Strongyloides ratti and Trichuris suis Affects Mucosal Homeostasis |
| title_fullStr | Multifunctional Thioredoxin-Like Protein from the Gastrointestinal Parasitic Nematodes Strongyloides ratti and Trichuris suis Affects Mucosal Homeostasis |
| title_full_unstemmed | Multifunctional Thioredoxin-Like Protein from the Gastrointestinal Parasitic Nematodes Strongyloides ratti and Trichuris suis Affects Mucosal Homeostasis |
| title_short | Multifunctional Thioredoxin-Like Protein from the Gastrointestinal Parasitic Nematodes Strongyloides ratti and Trichuris suis Affects Mucosal Homeostasis |
| title_sort | multifunctional thioredoxin like protein from the gastrointestinal parasitic nematodes strongyloides ratti and trichuris suis affects mucosal homeostasis |
| url | http://dx.doi.org/10.1155/2016/8421597 |
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