A simple, single-tube overlapping amplicon-targeted Illumina sequencing assay.
Targeted amplicon sequencing to identify pathogens, resistance-conferring mutations, and strain types is an important tool in diagnosing and treating infections. However, due to the short read limitations of Illumina sequencing, many applications require the splitting of limited clinical samples bet...
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| Main Authors: | Jason D Limberis, Alina Nalyvayko, Joel D Ernst, John Z Metcalfe |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2023-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0288687&type=printable |
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