Mechanistic study of the effect of a high-salt diet on the intestinal barrier
Abstract Despite the established link between chronic high salt diet (HSD) and an increase in gut inflammation, the effect of HSD on the integrity of the intestinal barrier remains understudied. The present study aims to investigate the impact of HSD on the intestinal barrier in rats, encompassing i...
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Nature Portfolio
2025-01-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-025-88291-y |
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| author | Li Chen Junrui Tang Yanglin Xia Jie Wang Li-na Xia |
| author_facet | Li Chen Junrui Tang Yanglin Xia Jie Wang Li-na Xia |
| author_sort | Li Chen |
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| description | Abstract Despite the established link between chronic high salt diet (HSD) and an increase in gut inflammation, the effect of HSD on the integrity of the intestinal barrier remains understudied. The present study aims to investigate the impact of HSD on the intestinal barrier in rats, encompassing its mechanical, mucous, and immune components. Expression levels of intestinal tight junction proteins and mucin-2 (MUC2) in SD rats were analyzed using immunofluorescence. The expression area of goblet cell mucopolysaccharides was assessed through PAS staining. Additionally, serum D-lactic acid, SIgA, β-defensin, and colonic tissue cytokines were measured using ELISA. Rats fed with HSD exhibited decreased expression of tight junction proteins, particularly Occludin, resulting in impairment of the intestinal epithelial barrier and an elevated serum D-lactic acid level. Furthermore, a notable reduction in the expression of goblet cell mucopolysaccharides, along with lower β-defensin and MUC2 levels, was observed. Notably, the SIgA and immune-related cytokines were significantly reduced in the HSD group. HSD disrupts the intestinal barrier in rats, leading to increased permeability and the entry of inflammatory factors into the bloodstream. This finding suggests that HSD may contribute to the pathogenesis of various diseases. |
| format | Article |
| id | doaj-art-7f5d24b409e74b439c97c2db49547936 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
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| series | Scientific Reports |
| spelling | doaj-art-7f5d24b409e74b439c97c2db495479362025-02-02T12:21:20ZengNature PortfolioScientific Reports2045-23222025-01-011511910.1038/s41598-025-88291-yMechanistic study of the effect of a high-salt diet on the intestinal barrierLi Chen0Junrui Tang1Yanglin Xia2Jie Wang3Li-na Xia4School of Health Preservation and Rehabilitation, Chengdu University of TCMSchool of Health Preservation and Rehabilitation, Chengdu University of TCMSchool of Health Preservation and Rehabilitation, Chengdu University of TCMSchool of Health Preservation and Rehabilitation, Chengdu University of TCMSchool of Health Preservation and Rehabilitation, Chengdu University of TCMAbstract Despite the established link between chronic high salt diet (HSD) and an increase in gut inflammation, the effect of HSD on the integrity of the intestinal barrier remains understudied. The present study aims to investigate the impact of HSD on the intestinal barrier in rats, encompassing its mechanical, mucous, and immune components. Expression levels of intestinal tight junction proteins and mucin-2 (MUC2) in SD rats were analyzed using immunofluorescence. The expression area of goblet cell mucopolysaccharides was assessed through PAS staining. Additionally, serum D-lactic acid, SIgA, β-defensin, and colonic tissue cytokines were measured using ELISA. Rats fed with HSD exhibited decreased expression of tight junction proteins, particularly Occludin, resulting in impairment of the intestinal epithelial barrier and an elevated serum D-lactic acid level. Furthermore, a notable reduction in the expression of goblet cell mucopolysaccharides, along with lower β-defensin and MUC2 levels, was observed. Notably, the SIgA and immune-related cytokines were significantly reduced in the HSD group. HSD disrupts the intestinal barrier in rats, leading to increased permeability and the entry of inflammatory factors into the bloodstream. This finding suggests that HSD may contribute to the pathogenesis of various diseases.https://doi.org/10.1038/s41598-025-88291-ySaltDietIntestinalBarrier functionIntestinal health |
| spellingShingle | Li Chen Junrui Tang Yanglin Xia Jie Wang Li-na Xia Mechanistic study of the effect of a high-salt diet on the intestinal barrier Scientific Reports Salt Diet Intestinal Barrier function Intestinal health |
| title | Mechanistic study of the effect of a high-salt diet on the intestinal barrier |
| title_full | Mechanistic study of the effect of a high-salt diet on the intestinal barrier |
| title_fullStr | Mechanistic study of the effect of a high-salt diet on the intestinal barrier |
| title_full_unstemmed | Mechanistic study of the effect of a high-salt diet on the intestinal barrier |
| title_short | Mechanistic study of the effect of a high-salt diet on the intestinal barrier |
| title_sort | mechanistic study of the effect of a high salt diet on the intestinal barrier |
| topic | Salt Diet Intestinal Barrier function Intestinal health |
| url | https://doi.org/10.1038/s41598-025-88291-y |
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