ANRIL regulates retinoblastoma progression via targeting autophagy by miR-328-3p/TSC1/ULK signaling
Retinoblastoma is the most common primary intraocular malignancy of childhood. The aim of our study was to investigate the role and regulatory mechanism of the long non-coding RNA ANRIL in retinoblastoma. Here, our data demonstrated that ANRIL overexpression inhibited miR-328-3p expression, but pr...
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| Format: | Article |
| Language: | English |
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Termedia Publishing House
2024-08-01
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| Series: | Polish Journal of Pathology |
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| Online Access: | https://www.termedia.pl/ANRIL-regulates-retinoblastoma-progression-via-targeting-autophagy-by-miR-328-3p-TSC1-ULK-signaling,55,54604,1,1.html |
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| _version_ | 1832584733677060096 |
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| author | Yang Yang Yuezhi Zhang Yanmei Fu Shuanglian Li Xiaolong Yin |
| author_facet | Yang Yang Yuezhi Zhang Yanmei Fu Shuanglian Li Xiaolong Yin |
| author_sort | Yang Yang |
| collection | DOAJ |
| description | Retinoblastoma is the most common primary intraocular malignancy of childhood. The aim of our study was to investigate the role and regulatory mechanism of the long non-coding RNA ANRIL in retinoblastoma.
Here, our data demonstrated that ANRIL overexpression inhibited miR-328-3p expression, but promoted expression of autophagy-related proteins (LC3B, ATG5, and BECN1). Then we predicted the binding sites for ANRIL with miR-328-3p, and for miR-328 3p with TSC1/ULK2 3′-UTR, and confirmed the combination of miR-328-3p and ANRIL and TSC1/ULK2 3′-UTR. Importantly, the data showed that ANRIL overexpression promoted TSC1 and ULK2 expression, and inhibited the phosphorylation of mTOR. Finally, our results indicated that ANRIL overexpression facilitated Y79 cell proliferation and cisplatin-induced apoptosis.
Our results indicated that ANRIL promoted the proliferation and cisplatin resistance of Y79 cells through activating autophagy by promoting TSC1/ULK2 ex- pression via acting as a miR-328-3p sponge. |
| format | Article |
| id | doaj-art-7f214da28c8e4e94ad0a9a7277d7a8f4 |
| institution | Kabale University |
| issn | 1233-9687 2084-9869 |
| language | English |
| publishDate | 2024-08-01 |
| publisher | Termedia Publishing House |
| record_format | Article |
| series | Polish Journal of Pathology |
| spelling | doaj-art-7f214da28c8e4e94ad0a9a7277d7a8f42025-01-27T11:36:07ZengTermedia Publishing HousePolish Journal of Pathology1233-96872084-98692024-08-0175322823510.5114/pjp.2024.14217754604ANRIL regulates retinoblastoma progression via targeting autophagy by miR-328-3p/TSC1/ULK signalingYang YangYuezhi ZhangYanmei FuShuanglian LiXiaolong YinRetinoblastoma is the most common primary intraocular malignancy of childhood. The aim of our study was to investigate the role and regulatory mechanism of the long non-coding RNA ANRIL in retinoblastoma. Here, our data demonstrated that ANRIL overexpression inhibited miR-328-3p expression, but promoted expression of autophagy-related proteins (LC3B, ATG5, and BECN1). Then we predicted the binding sites for ANRIL with miR-328-3p, and for miR-328 3p with TSC1/ULK2 3′-UTR, and confirmed the combination of miR-328-3p and ANRIL and TSC1/ULK2 3′-UTR. Importantly, the data showed that ANRIL overexpression promoted TSC1 and ULK2 expression, and inhibited the phosphorylation of mTOR. Finally, our results indicated that ANRIL overexpression facilitated Y79 cell proliferation and cisplatin-induced apoptosis. Our results indicated that ANRIL promoted the proliferation and cisplatin resistance of Y79 cells through activating autophagy by promoting TSC1/ULK2 ex- pression via acting as a miR-328-3p sponge.https://www.termedia.pl/ANRIL-regulates-retinoblastoma-progression-via-targeting-autophagy-by-miR-328-3p-TSC1-ULK-signaling,55,54604,1,1.htmlretinoblastoma competing endogenous rna long non-coding rna cisplatin resistance proliferation |
| spellingShingle | Yang Yang Yuezhi Zhang Yanmei Fu Shuanglian Li Xiaolong Yin ANRIL regulates retinoblastoma progression via targeting autophagy by miR-328-3p/TSC1/ULK signaling Polish Journal of Pathology retinoblastoma competing endogenous rna long non-coding rna cisplatin resistance proliferation |
| title | ANRIL regulates retinoblastoma progression via targeting autophagy by miR-328-3p/TSC1/ULK signaling |
| title_full | ANRIL regulates retinoblastoma progression via targeting autophagy by miR-328-3p/TSC1/ULK signaling |
| title_fullStr | ANRIL regulates retinoblastoma progression via targeting autophagy by miR-328-3p/TSC1/ULK signaling |
| title_full_unstemmed | ANRIL regulates retinoblastoma progression via targeting autophagy by miR-328-3p/TSC1/ULK signaling |
| title_short | ANRIL regulates retinoblastoma progression via targeting autophagy by miR-328-3p/TSC1/ULK signaling |
| title_sort | anril regulates retinoblastoma progression via targeting autophagy by mir 328 3p tsc1 ulk signaling |
| topic | retinoblastoma competing endogenous rna long non-coding rna cisplatin resistance proliferation |
| url | https://www.termedia.pl/ANRIL-regulates-retinoblastoma-progression-via-targeting-autophagy-by-miR-328-3p-TSC1-ULK-signaling,55,54604,1,1.html |
| work_keys_str_mv | AT yangyang anrilregulatesretinoblastomaprogressionviatargetingautophagybymir3283ptsc1ulksignaling AT yuezhizhang anrilregulatesretinoblastomaprogressionviatargetingautophagybymir3283ptsc1ulksignaling AT yanmeifu anrilregulatesretinoblastomaprogressionviatargetingautophagybymir3283ptsc1ulksignaling AT shuanglianli anrilregulatesretinoblastomaprogressionviatargetingautophagybymir3283ptsc1ulksignaling AT xiaolongyin anrilregulatesretinoblastomaprogressionviatargetingautophagybymir3283ptsc1ulksignaling |