Direct 3-D printing of complex optical phantoms using dynamic filament mixing

Abstract We report a method to directly 3-D print complex heterogeneous optical phantoms with programmable tissue-mimicking absorption and scattering properties. The proposed approach utilizes commercially available multi-color mixing extruders and off-the-shelf polylactic acid filaments, making thi...

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Bibliographic Details
Main Authors: Rahul Ragunathan, Miguel Mireles, Edward Xu, Aiden Lewis, Morris Vanegas, Qianqian Fang
Format: Article
Language:English
Published: Nature Portfolio 2025-03-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-025-94390-7
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Summary:Abstract We report a method to directly 3-D print complex heterogeneous optical phantoms with programmable tissue-mimicking absorption and scattering properties. The proposed approach utilizes commercially available multi-color mixing extruders and off-the-shelf polylactic acid filaments, making this technique low-cost and broadly accessible. We systematically characterized optical properties, including both absorption and reduced scattering coefficients, at a wide range of mixing ratios of gray, white, and translucent filaments and validated our hypothesis of a linear-mixing model between the filament mixing ratios and the resulting optical properties. Various techniques were used to design and fabricate sophisticated solid phantoms, including the design of color-purging towers, and the optimization of several printing parameters to improve print quality. To demonstrate the feasibility of this technique for generating anatomically complex phantoms with tunable optical absorption and scattering properties within tissue-relevant ranges, we designed and fabricated three heterogeneous optical phantoms. One of the presented phantoms was specifically designed to support quality assurance efforts in evaluating diffuse optics instruments and methodologies across various institutions. We have characterized the printed phantoms and observed an average error between 12%–15% compared to our linear-mixing model-predicted values. Future studies will target the usage of additional filament materials to expand potential imaging applications.
ISSN:2045-2322