A non‐fluorescent immunohistochemistry method for measuring autophagy flux using MAP1LC3/LC3 and SQSTM1 as core markers

A non‐fluorescent immunohistochemistry method for measuring autophagy flux using MAP1LC3/LC3 and SQSTM1 as core markers

Macroautophagy/autophagy is a crucial cellular process for degrading and recycling damaged proteins and organelles, playing a significant role in diseases such as cancer and neurodegeneration. Evaluating autophagy flux, which tracks autophagosome formation, maturation, and degradation, is essential...

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Bibliographic Details
Main Authors:	Shahla Shojaei, Amir Barzegar Behrooz, Marco Cordani, Mahmoud Aghaei, Negar Azarpira, Daniel J. Klionsky, Saeid Ghavami
Format:	Article
Language:	English
Published:	Wiley 2025-06-01
Series:	FEBS Open Bio
Subjects:	autophagometer autophagy flux measurement cellular homeostasis analysis chromogenic detection cost‐effective autophagy assay non‐fluorescent immunohistochemistry
Online Access:	https://doi.org/10.1002/2211-5463.70014
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