Design, Synthesis, and Antioxidant and Anti-Tyrosinase Activities of (<i>Z</i>)-5-Benzylidene-2-(naphthalen-1-ylamino)thiazol-4(5<i>H</i>)-one Analogs: In Vitro and In Vivo Insights
Fifteen compounds (<b>1</b>–<b>15</b>) constructed on a hybrid structure combining a β-phenyl-α,β-unsaturated carbonyl template and a 2-aminothiazol-4(5<i>H</i>)-one scaffold were designed and synthesized as potential novel anti-tyrosinase substances. Two compound...
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| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-01-01
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| Series: | Molecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1420-3049/30/2/289 |
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| Summary: | Fifteen compounds (<b>1</b>–<b>15</b>) constructed on a hybrid structure combining a β-phenyl-α,β-unsaturated carbonyl template and a 2-aminothiazol-4(5<i>H</i>)-one scaffold were designed and synthesized as potential novel anti-tyrosinase substances. Two compounds (<b>10</b> and <b>15</b>) showed more potent inhibition against mushroom tyrosinase than kojic acid, and the inhibitory activity of <b>10</b> (IC<sub>50</sub> value: 1.60 μM) was 11 times stronger than that of kojic acid. Lineweaver–Burk plots indicated that these two compounds were competitive inhibitors that bound to the mushroom tyrosinase active site, which was supported by in silico experiments. Compound <b>10</b> was an anti-tyrosinase and anti-melanogenic substance in B16F10 cells and was more potent than kojic acid, without cytotoxicity. Compound <b>15</b> exhibited the most potent effect on zebrafish larval depigmentation and showed a depigmentation effect comparable to kojic acid, even at a concentration 200 times lower. Compounds <b>8</b> and <b>10</b> exhibited strong antioxidant capacities, scavenging 2,2-diphenyl-1-picrylhydrazyl, (2,2-azino-bis-3-ethylbenzothiazoline-6-sulphonic acid)<sup>+</sup> radicals, and reactive oxygen species. Hybrid compounds <b>10</b> and <b>15</b> are potential therapeutic agents for skin hyperpigmentation disorders. |
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| ISSN: | 1420-3049 |