Analysis of cyt0kine gene expression in stimulated T cells of small children by semi-quantitative PCR
Only limited amounts of peripheral blood samples can be obtained from small children. Therefore, a polymerase chain reaction (PCR) aided analysis of cytokine gene expression by PBMC or T cells is a valuable tool. We present a combination of procedures to obtain an accurate estimation of the expressi...
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Main Authors: | H. Koning, M. R. M. Baert, R. de Groot, H. J. Neijens, H. F. J. Savelkoul |
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Format: | Article |
Language: | English |
Published: |
Wiley
1995-01-01
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Series: | Mediators of Inflammation |
Online Access: | http://dx.doi.org/10.1155/S0962935195000329 |
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