Bioinformatics approach reveals the critical role of inflammation-related genes in age-related hearing loss
Abstract Age-related hearing loss (ARHL) is the most prevalent sensory impairment in the elderly. However, the pathogenesis of ARHL remains unclear. This study was aimed to explore the potential inflammation-related genes of ARHL and suggest novel therapeutic targets for this condition. Initially, a...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2025-01-01
|
| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-024-83428-x |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832585851895283712 |
|---|---|
| author | Xi Gu Chenyu Chen Yuqing Chen Chaojun Zeng Yanchun Lin Ruosi Guo Shujin Xu Chang Lin |
| author_facet | Xi Gu Chenyu Chen Yuqing Chen Chaojun Zeng Yanchun Lin Ruosi Guo Shujin Xu Chang Lin |
| author_sort | Xi Gu |
| collection | DOAJ |
| description | Abstract Age-related hearing loss (ARHL) is the most prevalent sensory impairment in the elderly. However, the pathogenesis of ARHL remains unclear. This study was aimed to explore the potential inflammation-related genes of ARHL and suggest novel therapeutic targets for this condition. Initially, a total of 105 Inflammatory related differentially expressed genes (IRDEGs) were obtained by overlapping the differentially expressed genes from the GSE49522 and GSE49543 datasets with Inflammatory related genes. The IRDEGs were mainly enriched in MAPK, PI3K-Akt, Hippo and JAK-STAT pathways by analysis of Gene Ontology and Kyoto Encyclopedia of Genes and Genomes. We then identified 10 key IRDEGs including Alox5ap, Chil1, Clec7a, Dysf, Fcgr3, etc. using Least absolute shrinkage and selection operator regression analysis and converted them into human genes. The ROC curve indicated that Alox5ap expression presented a high accuracy in distinguishing between different groups. By CIBERSORT algorithm, 8 humanized key IRDEGs were correlated with the infiltration abundance of 3 immune cells. Finally, it showed that the Alox5ap expression was significantly more effective compared to other variables in the diagnostic model of ARHL. This study suggests that inflammation might play a role in the development of ARHL, providing a deeper understanding of the underlying causes of this disease. |
| format | Article |
| id | doaj-art-7d8bcc9ecfe2436bad1700a32eb9ae46 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-7d8bcc9ecfe2436bad1700a32eb9ae462025-01-26T12:28:10ZengNature PortfolioScientific Reports2045-23222025-01-0115111610.1038/s41598-024-83428-xBioinformatics approach reveals the critical role of inflammation-related genes in age-related hearing lossXi Gu0Chenyu Chen1Yuqing Chen2Chaojun Zeng3Yanchun Lin4Ruosi Guo5Shujin Xu6Chang Lin7Department of Otorhinolaryngology Head and Neck Surgery, The First Affiliated Hospital, Fujian Medical UniversityENT Institute, Department of Otorhinolaryngology, Eye & ENT Hospital, Fudan UniversityDepartment of Otorhinolaryngology Head and Neck Surgery, The First Affiliated Hospital, Fujian Medical UniversityDepartment of Otorhinolaryngology Head and Neck Surgery, The First Affiliated Hospital, Fujian Medical UniversityDepartment of Otorhinolaryngology Head and Neck Surgery, The First Affiliated Hospital, Fujian Medical UniversityFujian Medical UniversityFujian Medical UniversityDepartment of Otorhinolaryngology Head and Neck Surgery, The First Affiliated Hospital, Fujian Medical UniversityAbstract Age-related hearing loss (ARHL) is the most prevalent sensory impairment in the elderly. However, the pathogenesis of ARHL remains unclear. This study was aimed to explore the potential inflammation-related genes of ARHL and suggest novel therapeutic targets for this condition. Initially, a total of 105 Inflammatory related differentially expressed genes (IRDEGs) were obtained by overlapping the differentially expressed genes from the GSE49522 and GSE49543 datasets with Inflammatory related genes. The IRDEGs were mainly enriched in MAPK, PI3K-Akt, Hippo and JAK-STAT pathways by analysis of Gene Ontology and Kyoto Encyclopedia of Genes and Genomes. We then identified 10 key IRDEGs including Alox5ap, Chil1, Clec7a, Dysf, Fcgr3, etc. using Least absolute shrinkage and selection operator regression analysis and converted them into human genes. The ROC curve indicated that Alox5ap expression presented a high accuracy in distinguishing between different groups. By CIBERSORT algorithm, 8 humanized key IRDEGs were correlated with the infiltration abundance of 3 immune cells. Finally, it showed that the Alox5ap expression was significantly more effective compared to other variables in the diagnostic model of ARHL. This study suggests that inflammation might play a role in the development of ARHL, providing a deeper understanding of the underlying causes of this disease.https://doi.org/10.1038/s41598-024-83428-x |
| spellingShingle | Xi Gu Chenyu Chen Yuqing Chen Chaojun Zeng Yanchun Lin Ruosi Guo Shujin Xu Chang Lin Bioinformatics approach reveals the critical role of inflammation-related genes in age-related hearing loss Scientific Reports |
| title | Bioinformatics approach reveals the critical role of inflammation-related genes in age-related hearing loss |
| title_full | Bioinformatics approach reveals the critical role of inflammation-related genes in age-related hearing loss |
| title_fullStr | Bioinformatics approach reveals the critical role of inflammation-related genes in age-related hearing loss |
| title_full_unstemmed | Bioinformatics approach reveals the critical role of inflammation-related genes in age-related hearing loss |
| title_short | Bioinformatics approach reveals the critical role of inflammation-related genes in age-related hearing loss |
| title_sort | bioinformatics approach reveals the critical role of inflammation related genes in age related hearing loss |
| url | https://doi.org/10.1038/s41598-024-83428-x |
| work_keys_str_mv | AT xigu bioinformaticsapproachrevealsthecriticalroleofinflammationrelatedgenesinagerelatedhearingloss AT chenyuchen bioinformaticsapproachrevealsthecriticalroleofinflammationrelatedgenesinagerelatedhearingloss AT yuqingchen bioinformaticsapproachrevealsthecriticalroleofinflammationrelatedgenesinagerelatedhearingloss AT chaojunzeng bioinformaticsapproachrevealsthecriticalroleofinflammationrelatedgenesinagerelatedhearingloss AT yanchunlin bioinformaticsapproachrevealsthecriticalroleofinflammationrelatedgenesinagerelatedhearingloss AT ruosiguo bioinformaticsapproachrevealsthecriticalroleofinflammationrelatedgenesinagerelatedhearingloss AT shujinxu bioinformaticsapproachrevealsthecriticalroleofinflammationrelatedgenesinagerelatedhearingloss AT changlin bioinformaticsapproachrevealsthecriticalroleofinflammationrelatedgenesinagerelatedhearingloss |