Zika virus modulates human fibroblasts to enhance transmission success in a controlled lab-setting
Abstract Transmission of Zika virus (ZIKV) has been reported in 92 countries and the geographical spread of invasive virus-borne vectors has increased in recent years. Arboviruses naturally survive between vertebrate hosts and arthropod vectors. Transmission success requires the mosquito to feed on...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2025-01-01
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| Series: | Communications Biology |
| Online Access: | https://doi.org/10.1038/s42003-025-07543-9 |
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| author | Raimondas Mozūraitis Karsten Cirksena Mohammad Raftari Melika Hajkazemian Musa Mustapha Abiodun Juela Brahimi Sandra Radžiutė Violeta Apšegaitė Rasa Bernotienė Lech Ignatowicz Tessy Hick Andreas Kirschning Annasara Lenman Gisa Gerold S. Noushin Emami |
| author_facet | Raimondas Mozūraitis Karsten Cirksena Mohammad Raftari Melika Hajkazemian Musa Mustapha Abiodun Juela Brahimi Sandra Radžiutė Violeta Apšegaitė Rasa Bernotienė Lech Ignatowicz Tessy Hick Andreas Kirschning Annasara Lenman Gisa Gerold S. Noushin Emami |
| author_sort | Raimondas Mozūraitis |
| collection | DOAJ |
| description | Abstract Transmission of Zika virus (ZIKV) has been reported in 92 countries and the geographical spread of invasive virus-borne vectors has increased in recent years. Arboviruses naturally survive between vertebrate hosts and arthropod vectors. Transmission success requires the mosquito to feed on viraemic hosts. There is little specific understanding of factors that may promote ZIKV transmission-success. Here we show that mosquito host-seeking behaviour is impacted by viral infection of the vertebrae host and may be essential for the effective transmission of arboviruses like ZIKV. Human skin fibroblasts produce a variety of metabolites, and we show that ZIKV immediately alters gene/protein expression patterns in infected-dermal fibroblasts, altering their metabolism to increase the release of mosquito-attractive volatile organic compounds (VOCs), which improves its transmission success. We demonstrate that at the invasion stage, ZIKV differentially altered the emission of VOCs by significantly increasing or decreasing their amounts, while at the transmission stage of the virus, all VOCs are significantly increased. The findings are complemented by an extensive meta-proteome analysis. Overall, we demonstrate a multifaceted role of virus-host interaction and shed light on how arboviruses may influence the behaviour of their vectors as an evolved means of improving transmission-success. |
| format | Article |
| id | doaj-art-7d25e514ab5043dbb1e66ec751562e2b |
| institution | Kabale University |
| issn | 2399-3642 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Communications Biology |
| spelling | doaj-art-7d25e514ab5043dbb1e66ec751562e2b2025-02-02T12:37:12ZengNature PortfolioCommunications Biology2399-36422025-01-018111410.1038/s42003-025-07543-9Zika virus modulates human fibroblasts to enhance transmission success in a controlled lab-settingRaimondas Mozūraitis0Karsten Cirksena1Mohammad Raftari2Melika Hajkazemian3Musa Mustapha Abiodun4Juela Brahimi5Sandra Radžiutė6Violeta Apšegaitė7Rasa Bernotienė8Lech Ignatowicz9Tessy Hick10Andreas Kirschning11Annasara Lenman12Gisa Gerold13S. Noushin Emami14Department of Molecular Biosciences, Wenner-Gren Institute, Stockholm UniversityInstitute for Biochemistry & Research Center for Emerging Infections and Zoonoses (RIZ), University of Veterinary Medicine HanoverDepartment of Molecular Biosciences, Wenner-Gren Institute, Stockholm UniversityDepartment of Molecular Biosciences, Wenner-Gren Institute, Stockholm UniversityDepartment of Molecular Biosciences, Wenner-Gren Institute, Stockholm UniversityDepartment of Molecular Biosciences, Wenner-Gren Institute, Stockholm UniversityLaboratory of Chemical and Behavioral Ecology, Institute of Ecology, Nature Research CentreLaboratory of Chemical and Behavioral Ecology, Institute of Ecology, Nature Research CentreLaboratory of Entomology, Institute of Ecology, Nature Research CentreMolecular Attraction AB, Anderstorpsvägen 16, SolnaDepartment of Clinical Microbiology, Umeå UniversityInstitute of Organic Chemistry, Leibniz University Hannover, Schneiderberg 1BDepartment of Clinical Microbiology, Umeå UniversityInstitute for Biochemistry & Research Center for Emerging Infections and Zoonoses (RIZ), University of Veterinary Medicine HanoverDepartment of Molecular Biosciences, Wenner-Gren Institute, Stockholm UniversityAbstract Transmission of Zika virus (ZIKV) has been reported in 92 countries and the geographical spread of invasive virus-borne vectors has increased in recent years. Arboviruses naturally survive between vertebrate hosts and arthropod vectors. Transmission success requires the mosquito to feed on viraemic hosts. There is little specific understanding of factors that may promote ZIKV transmission-success. Here we show that mosquito host-seeking behaviour is impacted by viral infection of the vertebrae host and may be essential for the effective transmission of arboviruses like ZIKV. Human skin fibroblasts produce a variety of metabolites, and we show that ZIKV immediately alters gene/protein expression patterns in infected-dermal fibroblasts, altering their metabolism to increase the release of mosquito-attractive volatile organic compounds (VOCs), which improves its transmission success. We demonstrate that at the invasion stage, ZIKV differentially altered the emission of VOCs by significantly increasing or decreasing their amounts, while at the transmission stage of the virus, all VOCs are significantly increased. The findings are complemented by an extensive meta-proteome analysis. Overall, we demonstrate a multifaceted role of virus-host interaction and shed light on how arboviruses may influence the behaviour of their vectors as an evolved means of improving transmission-success.https://doi.org/10.1038/s42003-025-07543-9 |
| spellingShingle | Raimondas Mozūraitis Karsten Cirksena Mohammad Raftari Melika Hajkazemian Musa Mustapha Abiodun Juela Brahimi Sandra Radžiutė Violeta Apšegaitė Rasa Bernotienė Lech Ignatowicz Tessy Hick Andreas Kirschning Annasara Lenman Gisa Gerold S. Noushin Emami Zika virus modulates human fibroblasts to enhance transmission success in a controlled lab-setting Communications Biology |
| title | Zika virus modulates human fibroblasts to enhance transmission success in a controlled lab-setting |
| title_full | Zika virus modulates human fibroblasts to enhance transmission success in a controlled lab-setting |
| title_fullStr | Zika virus modulates human fibroblasts to enhance transmission success in a controlled lab-setting |
| title_full_unstemmed | Zika virus modulates human fibroblasts to enhance transmission success in a controlled lab-setting |
| title_short | Zika virus modulates human fibroblasts to enhance transmission success in a controlled lab-setting |
| title_sort | zika virus modulates human fibroblasts to enhance transmission success in a controlled lab setting |
| url | https://doi.org/10.1038/s42003-025-07543-9 |
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