Comparison of Lipid Content in Nine Dinoflagellate Species Using Flow Cytometry
The lipid content of nine dinoflagellates was analyzed using flow cytometry to compare lipid levels. Additionally, the correlation between lipid content, cell size, and carbon content in dinoflagellates was evaluated using BODIPY 505/515 staining. The flow cytometry side scatter (SSC) effectively re...
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MDPI AG
2024-12-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/13/1/44 |
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| author | Jaeyeon Park Eun Young Yoon Seung Joo Moon Jun-Ho Hyung Hangy Lee |
| author_facet | Jaeyeon Park Eun Young Yoon Seung Joo Moon Jun-Ho Hyung Hangy Lee |
| author_sort | Jaeyeon Park |
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| description | The lipid content of nine dinoflagellates was analyzed using flow cytometry to compare lipid levels. Additionally, the correlation between lipid content, cell size, and carbon content in dinoflagellates was evaluated using BODIPY 505/515 staining. The flow cytometry side scatter (SSC) effectively represented relative cell size, showing a linear relationship with the equivalent spherical diameter (ESD). Larger cells exhibited higher relative lipid content; however, lipid accumulation was influenced by nutritional modes and habitats, with mixorophic and benthic species displaying higher lipid content than heterotrophic species. A comparison of fluorescent dyes revealed that Nile Red overestimated lipid content, suggesting overlap with chlorophyll autofluorescence. Flow cytometry analysis with BODIPY 505/515 demonstrated a linear correlation with the sulfo-phospho-vanillin (SPV) method, enabling determination of actual lipid content using FL1 fluorescence and the slope value. As the carbon content increased, the lipid content initially increased rapidly but plateaued at higher carbon levels, indicating saturation. These findings suggest that relative fluorescence via flow cytometry provides an effective means to estimate the lipid content and carbon content as a function of cell size. |
| format | Article |
| id | doaj-art-7c32953eeb8748f4b72d772275a93fe4 |
| institution | Kabale University |
| issn | 2076-2607 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
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| series | Microorganisms |
| spelling | doaj-art-7c32953eeb8748f4b72d772275a93fe42025-01-24T13:42:24ZengMDPI AGMicroorganisms2076-26072024-12-011314410.3390/microorganisms13010044Comparison of Lipid Content in Nine Dinoflagellate Species Using Flow CytometryJaeyeon Park0Eun Young Yoon1Seung Joo Moon2Jun-Ho Hyung3Hangy Lee4Advanced Institute of Convergence Technology, Suwon 16229, Republic of KoreaAdvanced Institute of Convergence Technology, Suwon 16229, Republic of KoreaAdvanced Institute of Convergence Technology, Suwon 16229, Republic of KoreaAdvanced Institute of Convergence Technology, Suwon 16229, Republic of KoreaAdvanced Institute of Convergence Technology, Suwon 16229, Republic of KoreaThe lipid content of nine dinoflagellates was analyzed using flow cytometry to compare lipid levels. Additionally, the correlation between lipid content, cell size, and carbon content in dinoflagellates was evaluated using BODIPY 505/515 staining. The flow cytometry side scatter (SSC) effectively represented relative cell size, showing a linear relationship with the equivalent spherical diameter (ESD). Larger cells exhibited higher relative lipid content; however, lipid accumulation was influenced by nutritional modes and habitats, with mixorophic and benthic species displaying higher lipid content than heterotrophic species. A comparison of fluorescent dyes revealed that Nile Red overestimated lipid content, suggesting overlap with chlorophyll autofluorescence. Flow cytometry analysis with BODIPY 505/515 demonstrated a linear correlation with the sulfo-phospho-vanillin (SPV) method, enabling determination of actual lipid content using FL1 fluorescence and the slope value. As the carbon content increased, the lipid content initially increased rapidly but plateaued at higher carbon levels, indicating saturation. These findings suggest that relative fluorescence via flow cytometry provides an effective means to estimate the lipid content and carbon content as a function of cell size.https://www.mdpi.com/2076-2607/13/1/44dinoflagellatelipid contentflow cytometrycarbon contentcell size |
| spellingShingle | Jaeyeon Park Eun Young Yoon Seung Joo Moon Jun-Ho Hyung Hangy Lee Comparison of Lipid Content in Nine Dinoflagellate Species Using Flow Cytometry Microorganisms dinoflagellate lipid content flow cytometry carbon content cell size |
| title | Comparison of Lipid Content in Nine Dinoflagellate Species Using Flow Cytometry |
| title_full | Comparison of Lipid Content in Nine Dinoflagellate Species Using Flow Cytometry |
| title_fullStr | Comparison of Lipid Content in Nine Dinoflagellate Species Using Flow Cytometry |
| title_full_unstemmed | Comparison of Lipid Content in Nine Dinoflagellate Species Using Flow Cytometry |
| title_short | Comparison of Lipid Content in Nine Dinoflagellate Species Using Flow Cytometry |
| title_sort | comparison of lipid content in nine dinoflagellate species using flow cytometry |
| topic | dinoflagellate lipid content flow cytometry carbon content cell size |
| url | https://www.mdpi.com/2076-2607/13/1/44 |
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