Genome-Wide Approach Identifies Natural Large-Fragment Deletion in ASFV Strains Circulating in Italy During 2023
African swine fever (ASF), characterized by high mortality rates in infected animals, remains a significant global veterinary and economic concern, due to the widespread distribution of ASF virus (ASFV) genotype II across five continents. In this study, ASFV strains collected in Italy during 2022–20...
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MDPI AG
2025-01-01
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| author | Claudia Torresi Roberta Biccheri Cesare Cammà Carmina Gallardo Maurilia Marcacci Simona Zoppi Barbara Secondini Caterina Riverso Alejandro Soler Cristina Casciari Michela Pela Elisabetta Rossi Claudia Pellegrini Carmen Iscaro Francesco Feliziani Monica Giammarioli |
| author_facet | Claudia Torresi Roberta Biccheri Cesare Cammà Carmina Gallardo Maurilia Marcacci Simona Zoppi Barbara Secondini Caterina Riverso Alejandro Soler Cristina Casciari Michela Pela Elisabetta Rossi Claudia Pellegrini Carmen Iscaro Francesco Feliziani Monica Giammarioli |
| author_sort | Claudia Torresi |
| collection | DOAJ |
| description | African swine fever (ASF), characterized by high mortality rates in infected animals, remains a significant global veterinary and economic concern, due to the widespread distribution of ASF virus (ASFV) genotype II across five continents. In this study, ASFV strains collected in Italy during 2022–2023 from two geographical clusters, North-West (Alessandria) and Calabria, were fully sequenced. In addition, an in vivo experiment in pigs was performed. Complete genomic sequencing of 30 strains revealed large-fragment deletions and translocations. In Alessandria, five samples showed two different deletions in the 5′ genomic region: a ~4340 bp deletion (positions ~9020–13,356 in Georgia 2007/1) in four samples and a 2162 bp deletion (positions 17,837–19,998) in one sample. Another strain showed a truncation of 1950 bp at the 3′ end. In Calabria, strains showed a 5137 bp deletion (positions 10,755–15,891) and ~2 kb truncations in the 3′ region. Two strains showed a translocation from the region 1–2244 to positions 188,631–190,584. In vivo characterization of the deleted strain 22489.4_2312/RC/2023 revealed identical disease progression to the wild-type strain, with severe ASF symptoms in inoculated pigs. This study is the first to report natural deleted strains of ASFV in Italy, revealing unique genomic deletions distinct from those in previously known strains. |
| format | Article |
| id | doaj-art-7bfc516b4c724ab2afd3aaee9ab40a3e |
| institution | Kabale University |
| issn | 2076-0817 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj-art-7bfc516b4c724ab2afd3aaee9ab40a3e2025-01-24T13:44:43ZengMDPI AGPathogens2076-08172025-01-011415110.3390/pathogens14010051Genome-Wide Approach Identifies Natural Large-Fragment Deletion in ASFV Strains Circulating in Italy During 2023Claudia Torresi0Roberta Biccheri1Cesare Cammà2Carmina Gallardo3Maurilia Marcacci4Simona Zoppi5Barbara Secondini6Caterina Riverso7Alejandro Soler8Cristina Casciari9Michela Pela10Elisabetta Rossi11Claudia Pellegrini12Carmen Iscaro13Francesco Feliziani14Monica Giammarioli15National Reference Laboratory (NRL) for Swine Fever, Istituto Zooprofilattico Sperimentale dell’ Umbria e delle Marche “Togo Rosati”, 06126 Perugia, ItalyNational Reference Laboratory (NRL) for Swine Fever, Istituto Zooprofilattico Sperimentale dell’ Umbria e delle Marche “Togo Rosati”, 06126 Perugia, ItalyNational Reference Center for Whole Genome Sequencing of Microbial Pathogens, Database and Bioinformatic Analysis (GENPAT), Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise “G. Caporale”, 64100 Teramo, ItalyEuropean Union Reference Laboratory for African swine fever (EURL), Centro de Investigación en Sanidad Animal (CISA), Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Consejo Superior de Investigaciones Científicas (CSIC), Valdeolmos, 28130 Madrid, SpainNational Reference Center for Whole Genome Sequencing of Microbial Pathogens, Database and Bioinformatic Analysis (GENPAT), Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise “G. Caporale”, 64100 Teramo, ItalyIstituto Zooprofilattico Sperimentale Piemonte, Liguria e Valle d’Aosta “I. Altara”, 10154 Turin, ItalyNational Reference Center for Whole Genome Sequencing of Microbial Pathogens, Database and Bioinformatic Analysis (GENPAT), Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise “G. Caporale”, 64100 Teramo, ItalyIstituto Zooprofilattico Sperimentale del Mezzogiorno, Portici, 80055 Napoli, ItalyEuropean Union Reference Laboratory for African swine fever (EURL), Centro de Investigación en Sanidad Animal (CISA), Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Consejo Superior de Investigaciones Científicas (CSIC), Valdeolmos, 28130 Madrid, SpainNational Reference Laboratory (NRL) for Swine Fever, Istituto Zooprofilattico Sperimentale dell’ Umbria e delle Marche “Togo Rosati”, 06126 Perugia, ItalyNational Reference Laboratory (NRL) for Swine Fever, Istituto Zooprofilattico Sperimentale dell’ Umbria e delle Marche “Togo Rosati”, 06126 Perugia, ItalyNational Reference Laboratory (NRL) for Swine Fever, Istituto Zooprofilattico Sperimentale dell’ Umbria e delle Marche “Togo Rosati”, 06126 Perugia, ItalyNational Reference Laboratory (NRL) for Swine Fever, Istituto Zooprofilattico Sperimentale dell’ Umbria e delle Marche “Togo Rosati”, 06126 Perugia, ItalyNational Reference Laboratory (NRL) for Swine Fever, Istituto Zooprofilattico Sperimentale dell’ Umbria e delle Marche “Togo Rosati”, 06126 Perugia, ItalyNational Reference Laboratory (NRL) for Swine Fever, Istituto Zooprofilattico Sperimentale dell’ Umbria e delle Marche “Togo Rosati”, 06126 Perugia, ItalyNational Reference Laboratory (NRL) for Swine Fever, Istituto Zooprofilattico Sperimentale dell’ Umbria e delle Marche “Togo Rosati”, 06126 Perugia, ItalyAfrican swine fever (ASF), characterized by high mortality rates in infected animals, remains a significant global veterinary and economic concern, due to the widespread distribution of ASF virus (ASFV) genotype II across five continents. In this study, ASFV strains collected in Italy during 2022–2023 from two geographical clusters, North-West (Alessandria) and Calabria, were fully sequenced. In addition, an in vivo experiment in pigs was performed. Complete genomic sequencing of 30 strains revealed large-fragment deletions and translocations. In Alessandria, five samples showed two different deletions in the 5′ genomic region: a ~4340 bp deletion (positions ~9020–13,356 in Georgia 2007/1) in four samples and a 2162 bp deletion (positions 17,837–19,998) in one sample. Another strain showed a truncation of 1950 bp at the 3′ end. In Calabria, strains showed a 5137 bp deletion (positions 10,755–15,891) and ~2 kb truncations in the 3′ region. Two strains showed a translocation from the region 1–2244 to positions 188,631–190,584. In vivo characterization of the deleted strain 22489.4_2312/RC/2023 revealed identical disease progression to the wild-type strain, with severe ASF symptoms in inoculated pigs. This study is the first to report natural deleted strains of ASFV in Italy, revealing unique genomic deletions distinct from those in previously known strains.https://www.mdpi.com/2076-0817/14/1/51African swine fever virusgenotype IInatural deleted strainslarge-fragment deletionexperimental infection |
| spellingShingle | Claudia Torresi Roberta Biccheri Cesare Cammà Carmina Gallardo Maurilia Marcacci Simona Zoppi Barbara Secondini Caterina Riverso Alejandro Soler Cristina Casciari Michela Pela Elisabetta Rossi Claudia Pellegrini Carmen Iscaro Francesco Feliziani Monica Giammarioli Genome-Wide Approach Identifies Natural Large-Fragment Deletion in ASFV Strains Circulating in Italy During 2023 Pathogens African swine fever virus genotype II natural deleted strains large-fragment deletion experimental infection |
| title | Genome-Wide Approach Identifies Natural Large-Fragment Deletion in ASFV Strains Circulating in Italy During 2023 |
| title_full | Genome-Wide Approach Identifies Natural Large-Fragment Deletion in ASFV Strains Circulating in Italy During 2023 |
| title_fullStr | Genome-Wide Approach Identifies Natural Large-Fragment Deletion in ASFV Strains Circulating in Italy During 2023 |
| title_full_unstemmed | Genome-Wide Approach Identifies Natural Large-Fragment Deletion in ASFV Strains Circulating in Italy During 2023 |
| title_short | Genome-Wide Approach Identifies Natural Large-Fragment Deletion in ASFV Strains Circulating in Italy During 2023 |
| title_sort | genome wide approach identifies natural large fragment deletion in asfv strains circulating in italy during 2023 |
| topic | African swine fever virus genotype II natural deleted strains large-fragment deletion experimental infection |
| url | https://www.mdpi.com/2076-0817/14/1/51 |
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