A single-plasmid-based, easily curable CRISPR/Cas9 system for rapid, iterative genome editing in Pseudomonas putida KT2440

A single-plasmid-based, easily curable CRISPR/Cas9 system for rapid, iterative genome editing in Pseudomonas putida KT2440

Abstract Background Pseudomonas putida KT2440, a non-pathogenic soil bacterium, is a key platform strain in synthetic biology and industrial applications due to its robustness and metabolic versatility. Various systems have been developed for genome editing in P. putida, including transposon modules...

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Bibliographic Details
Main Authors: Qifeng Wen, JinJin Chen, Jin Li, Ida Putu Wiweka Dharmasiddhi, Maohua Yang, Jianmin Xing, Yilan Liu
Format: Article
Language:English
Published: BMC 2024-12-01
Series:Microbial Cell Factories
Subjects:
Pseudomonas putida KT2440
All-in-one CRISPR/Cas9 system
Plasmid curing
Genome editing
Biosynthesis
Online Access:https://doi.org/10.1186/s12934-024-02634-4
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https://doi.org/10.1186/s12934-024-02634-4

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