Neutrophil adhesion to vessel walls impairs pulmonary circulation in COVID-19 pathology
Abstract Microthrombus formation is associated with COVID-19 severity; however, the detailed mechanism remains unclear. In this study, we investigated mouse models with severe pneumonia caused by SARS-CoV-2 infection by using our in vivo two-photon imaging system. In the lungs of SARS-CoV-2-infected...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2025-01-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-024-55272-0 |
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| author | Hiroshi Ueki I-Hsuan Wang Maki Kiso Kenta Horie Shun Iida Sohtaro Mine Michiko Ujie Hung-Wei Hsu Chen-Hui Henry Wu Masaki Imai Tadaki Suzuki Wataru Kamitani Eiryo Kawakami Yoshihiro Kawaoka |
| author_facet | Hiroshi Ueki I-Hsuan Wang Maki Kiso Kenta Horie Shun Iida Sohtaro Mine Michiko Ujie Hung-Wei Hsu Chen-Hui Henry Wu Masaki Imai Tadaki Suzuki Wataru Kamitani Eiryo Kawakami Yoshihiro Kawaoka |
| author_sort | Hiroshi Ueki |
| collection | DOAJ |
| description | Abstract Microthrombus formation is associated with COVID-19 severity; however, the detailed mechanism remains unclear. In this study, we investigated mouse models with severe pneumonia caused by SARS-CoV-2 infection by using our in vivo two-photon imaging system. In the lungs of SARS-CoV-2-infected mice, increased expression of adhesion molecules in intravascular neutrophils prolonged adhesion time to the vessel wall, resulting in platelet aggregation and impaired lung perfusion. Re-analysis of scRNA-seq data from peripheral blood mononuclear cells from COVID-19 cases revealed increased expression levels of CD44 and SELL in neutrophils in severe COVID-19 cases compared to a healthy group, consistent with our observations in the mouse model. These findings suggest that pulmonary perfusion defects caused by neutrophil adhesion to pulmonary vessels contribute to COVID-19 severity. |
| format | Article |
| id | doaj-art-7b2e63d4a60a45ee881b5cd994296751 |
| institution | Kabale University |
| issn | 2041-1723 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj-art-7b2e63d4a60a45ee881b5cd9942967512025-01-19T12:29:47ZengNature PortfolioNature Communications2041-17232025-01-0116111710.1038/s41467-024-55272-0Neutrophil adhesion to vessel walls impairs pulmonary circulation in COVID-19 pathologyHiroshi Ueki0I-Hsuan Wang1Maki Kiso2Kenta Horie3Shun Iida4Sohtaro Mine5Michiko Ujie6Hung-Wei Hsu7Chen-Hui Henry Wu8Masaki Imai9Tadaki Suzuki10Wataru Kamitani11Eiryo Kawakami12Yoshihiro Kawaoka13Division of Virology, Institute of Medical Science, University of TokyoInstitute of Biomedical Sciences, Academia SinicaDivision of Virology, Institute of Medical Science, University of TokyoDepartment of Artificial Intelligence Medicine, Graduate School of Medicine, Chiba UniversityDepartment of Pathology, National Institute of Infectious DiseasesDepartment of Pathology, National Institute of Infectious DiseasesDivision of Virology, Institute of Medical Science, University of TokyoInstitute of Biomedical Sciences, Academia SinicaInstitute of Biomedical Sciences, Academia SinicaDivision of Virology, Institute of Medical Science, University of TokyoDepartment of Pathology, National Institute of Infectious DiseasesDepartment of Infectious Diseases and Host Defense, Gunma University Graduate School of MedicineDepartment of Artificial Intelligence Medicine, Graduate School of Medicine, Chiba UniversityDivision of Virology, Institute of Medical Science, University of TokyoAbstract Microthrombus formation is associated with COVID-19 severity; however, the detailed mechanism remains unclear. In this study, we investigated mouse models with severe pneumonia caused by SARS-CoV-2 infection by using our in vivo two-photon imaging system. In the lungs of SARS-CoV-2-infected mice, increased expression of adhesion molecules in intravascular neutrophils prolonged adhesion time to the vessel wall, resulting in platelet aggregation and impaired lung perfusion. Re-analysis of scRNA-seq data from peripheral blood mononuclear cells from COVID-19 cases revealed increased expression levels of CD44 and SELL in neutrophils in severe COVID-19 cases compared to a healthy group, consistent with our observations in the mouse model. These findings suggest that pulmonary perfusion defects caused by neutrophil adhesion to pulmonary vessels contribute to COVID-19 severity.https://doi.org/10.1038/s41467-024-55272-0 |
| spellingShingle | Hiroshi Ueki I-Hsuan Wang Maki Kiso Kenta Horie Shun Iida Sohtaro Mine Michiko Ujie Hung-Wei Hsu Chen-Hui Henry Wu Masaki Imai Tadaki Suzuki Wataru Kamitani Eiryo Kawakami Yoshihiro Kawaoka Neutrophil adhesion to vessel walls impairs pulmonary circulation in COVID-19 pathology Nature Communications |
| title | Neutrophil adhesion to vessel walls impairs pulmonary circulation in COVID-19 pathology |
| title_full | Neutrophil adhesion to vessel walls impairs pulmonary circulation in COVID-19 pathology |
| title_fullStr | Neutrophil adhesion to vessel walls impairs pulmonary circulation in COVID-19 pathology |
| title_full_unstemmed | Neutrophil adhesion to vessel walls impairs pulmonary circulation in COVID-19 pathology |
| title_short | Neutrophil adhesion to vessel walls impairs pulmonary circulation in COVID-19 pathology |
| title_sort | neutrophil adhesion to vessel walls impairs pulmonary circulation in covid 19 pathology |
| url | https://doi.org/10.1038/s41467-024-55272-0 |
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